Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
 
(88 intermediate revisions not shown)
Line 1: Line 1:
 +
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
-
<head>
+
<head>
-
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<meta name="generator" content="MediaWiki 1.16.5" />
+
<meta name="generator" content="MediaWiki 1.16.5" />
-
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea&amp;action=edit" />
+
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea&amp;action=edit" />
+
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
<link rel="shortcut icon" href="/favicon.ico" />
<link rel="shortcut icon" href="/favicon.ico" />
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
-
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea&amp;action=creativecommons" rel="meta" />
+
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-
<link rel="alternate" type="application/atom+xml" title="2013.igem.org Atom feed" href="/wiki/index.php?title=Special:RecentChanges&amp;feed=atom" /> <title>Team:KAIST_Korea - 2013.igem.org</title>
+
<meta name="viewport" content="width=device-width, initial-scale=1.0 minimum-scale=1, maximum-scale=1, user-scalable=yes, target-densitydpi=medium-dpi" />
-
<style type="text/css" media="screen, projection">/*<![CDATA[*/
+
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic' rel='stylesheet' type='text/css'>
 +
<title>Team:KAIST Korea/Project Background - 2013.igem.org</title>
 +
<style type="text/css" media="screen, projection">/*<![CDATA[*/
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/igem/main.css?270";
@import "/wiki/skins/igem/main.css?270";
-
/*]]>*/</style>
+
#bar{z-index : 5;
-
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
+
}
-
<!--[if lt IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE50Fixes.css?270";</style><![endif]-->
+
#menu,
-
<!--[if IE 5.5000]><style type="text/css">@import "/wiki/skins/igem/IE55Fixes.css?270";</style><![endif]-->
+
#menu ul,
-
<!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</style><![endif]-->
+
#menu li,
-
<!--[if IE 7]><style type="text/css">@import "/wiki/skins/igem/IE70Fixes.css?270";</style><![endif]-->
+
#menu a {
-
<!--[if lt IE 7]><script type="text/javascript" src="/wiki/skins/common/IEFixes.js?270"></script>
+
margin: 0;
-
<meta http-equiv="imagetoolbar" content="no" /><![endif]-->
+
padding-left: 40;
 +
border: none;
 +
outline: none;
 +
}
 +
.menu {
 +
height: 50px;
 +
weight: 100%;
 +
padding-left: 30%;
 +
        z-index: 2;
 +
background: #000000;
 +
}
 +
 +
.menu li {
 +
position: relative;
 +
list-style: none;
 +
margin: 0px auto 0 auto;
 +
float: left;
 +
display: block;
 +
height: 50px;
 +
}
 +
 +
/* Links */
 +
 +
.menu li a {
 +
margin:0px auto;
 +
display: block;
 +
padding: 0 14px;
 +
margin: 6px 0;
 +
 +
text-decoration: none;
 +
 +
 
 +
 +
font-family: Helvetica, Arial, sans-serif;
 +
font-weight: bold;
 +
font-size: 20px;
 +
 +
color: #353535;
 +
 +
 +
-webkit-transition: color .2s ease-in-out;
 +
-moz-transition: color .2s ease-in-out;
 +
-o-transition: color .2s ease-in-out;
 +
-ms-transition: color .2s ease-in-out;
 +
transition: color .2s ease-in-out;
 +
}
 +
 +
.menu li:first-child a { border-left: none; }
 +
.menu li:last-child a{ border-right: none; }
 +
 +
.menu li:hover > a { color: #F29661; }
 +
 +
/* Sub Menu */
 +
 +
.menu ul {
 +
position: absolute;
 +
top: 50px;
 +
 +
left: 0px;
 +
 +
padding-right: 10px;
 +
 +
opacity: 0;
 +
 +
background: #1f2024;
 +
 +
-webkit-border-radius: 0 0 5px 5px;
 +
-moz-border-radius: 0 0 5px 5px;
 +
border-radius: 0 0 5px 5px;
 +
 +
-webkit-transition: opacity .25s ease .1s;
 +
-moz-transition: opacity .25s ease .1s;
 +
-o-transition: opacity .25s ease .1s;
 +
-ms-transition: opacity .25s ease .1s;
 +
transition: opacity .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul { opacity: 1; }
 +
 +
.menu ul li {
 +
height: 0;
 +
overflow: hidden;
 +
padding: 0px;
 +
 +
-webkit-transition: height .25s ease .1s;
 +
-moz-transition: height .25s ease .1s;
 +
-o-transition: height .25s ease .1s;
 +
-ms-transition: height .25s ease .1s;
 +
transition: height .25s ease .1s;
 +
}
 +
 +
.menu li:hover > ul li {
 +
height: 40px;
 +
overflow: visible;
 +
padding: 0;
 +
}
 +
 +
.menu ul li a {
 +
width: 100px;
 +
padding: 4px 0 4px 40px;
 +
margin: 0;
 +
 +
border: none;
 +
border-bottom: 1px solid #353539;
 +
}
 +
 +
.menu ul li:last-child a { border: none; }
 +
 +
/* Icons */
 +
 +
.menu a.documents { background: url(docs.png) no-repeat 6px center; }
 +
.menu a.messages { background: url(bubble.png) no-repeat 6px center; }
 +
.menu a.signout { background: url(arrow.png) no-repeat 6px center; }
 +
 +
 +
ul.menu { margin: 0px auto 0 auto; }
 +
</style>
 +
 
 +
<style type="text/css">
-
<script>
+
/* Cross-Browsing */
 +
html,body,div,ul,ol,li,dl,dt,dd,h1,h2,h3,h4,h5,h6,pre,form,p,blockquote,fieldset,input,hr {margin:0; padding:0;}
 +
h1,h2,h3,h4,h5,h6,pre,code,address,caption,cite,code,em,strong,th {font-size:1em; font-weight:normal; font-style:normal;}
 +
ol, ul, li { list-style:none; }
 +
fieldset,img,hr {border:none;}
 +
caption,th {text-align:left;}
 +
table {border-collapse:collapse; border-spacing:0;}
 +
td {vertical-align:top;}
 +
strong { font-weight:bold ;}
 +
em { font-style:italic; }
 +
a img { border:none; }
 +
q:before, q:after { content:''}
 +
 +
html { height: 100%; }
 +
 +
 +
body
 +
{
 +
background-image: url('https://static.igem.org/mediawiki/2012/6/61/Top_section_expand_KAIST.png'),url('https://static.igem.org/mediawiki/2012/9/98/PATTERN_diagonal.png');
 +
background-repeat: repeat-x,repeat;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
 +
 +
#content
 +
{
 +
border: none;
 +
position: relative;
 +
top: 0;
 +
background-color: transparent;
 +
padding: 0;
 +
width: 100%;
 +
}
 +
 +
#bodyContent p
 +
{
 +
display: none;
 +
}
 +
 +
#globalWrapper
 +
{
 +
clear: both;
 +
width: 100%;
 +
                z-index: 1;
 +
position: relative;
 +
top:0px;
 +
margin: auto;
 +
font-size: 10px;
 +
}
 +
 +
#contentSub { display: none; }
 +
#siteSub { display:none; }
 +
 +
#search-controls {
 +
left: 20px;
 +
width: auto;
 +
        z-index: 100;
 +
        position: relative;
 +
        float: right;
 +
        top: -10px;
 +
        right: 0;
 +
        height: auto;
 +
        clear: both;
 +
}
 +
 +
input.searchButton
 +
{
 +
position: relative;
 +
font-size: 1em;
 +
font-family: "Arial";
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 +
#searchInput
 +
{
 +
background-color: transparent;
 +
z-index: 100;
 +
color: #181818;
 +
font: Arial;
 +
text-align: center;
 +
 +
background: url('https://static.igem.org/mediawiki/2012/6/6e/Kaist2012_head_searchbar.png') no-repeat;
 +
background-position: 0px 0px;
 +
vertical-align: middle;
 +
 +
width: 184px;
 +
height: 22px;
 +
border: none;
 +
}
 +
 +
#searchGoButton
 +
{
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/7/78/Kaist2012_head_arrow.png') no-repeat;
 +
background-position: left 0px;
 +
        z-index: 100;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
#mw-searchButton
 +
{
 +
position: relative;
 +
left: -14px;
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
 +
                z-index: 100;
 +
background-position: left 0px;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
.firstHeading { display:none; }
 +
 +
#p-logo
 +
{
 +
display: none;
 +
position: relative;
 +
top: 0px;
 +
z-index: 0;
 +
height: 1px;
 +
width: 10px;
 +
overflow: hidden;
 +
}
 +
 +
#p-logo a { color: black; }
 +
 +
img[src*="/wiki/skins/common/images/wiki.png"]
 +
{
 +
    visibility: hidden;
 +
    top: -100px;
 +
    width: 10px;
 +
    height: 0px;
 +
margin-top: 20px;
 +
    background: transparent;
 +
}
 +
 +
 +
 +
#menubar
 +
{
 +
font-size: 10px;
 +
font-family: "GothamXNarrow-Bold", "Helvetica";
 +
position: relative;
 +
float: left;
 +
width: auto;
 +
top: 36px;
 +
        z-index: 100;
 +
}
 +
 +
#menubar:hover{ background-color: #181818; }
 +
 +
.left-menu { margin-top: 0px; left: 0; }
 +
.left-menu ul { border: none;}
 +
.right-menu { margin-top: 0px; right: 0; float: right !important;}
 +
.right-menu ul { border: none; }
 +
.right-menu ul a { background-color: transparent; }
 +
.left-menu li a { color: #615d5a; }
 +
.left-menu li a:visited { color: #615d5a; }
 +
.left-menu li a:hover { color: #ffffff; }
 +
.left-menu li a:active{ color: #cccccc; font-weight: bold; }
 +
.right-menu li a { color: #f6f5c8; }
 +
.right-menu li a:visited { color: #f6f5c8; }
 +
.right-menu li a:hover { color: #ffffff; text-decoration: none; }
 +
.right-menu li a:active { color: #cccccc; text-decoration: none;font-weight: bold; }
 +
 +
#menubar li {
 +
 +
        margin: 0px;
 +
        word-spacing:-1px;
 +
}
 +
 +
#catlinks {
 +
position: relative;
 +
top: 5px;
 +
left: -5px;
 +
width: 1034px;
 +
border: none;
 +
background-color: transparent;
 +
padding-top: 5px;
 +
clear: both;
 +
}
 +
 +
#footer-box {display:none;}
 +
#footer {display:none;}
 +
#footer li a { color: #000000; }
 +
#footer img { margin: 10px; border: none; }
 +
#footer li a:visited {color: #000000;}
 +
#footer li a:hover { color: #ababab; text-decoration: none; }
 +
#footer li a:active{ color: #ababab; text-decoration: none; font-weight: bold}
 +
        </style>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic);
 +
ul.oe_menu{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
 +
 +
}
 +
 +
ul.oe_menu > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#aaa;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu > li > a:hover,
 +
ul.oe_menu > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
.oe_wrapper ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
ul.oe_menu div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu div ul li a{
 +
text-decoration:none;
 +
color:#cbcbcb;
 +
padding:2px 2px 2px 4px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
ul.oe_menu div ul.oe_full{
 +
width:100%;
 +
}
 +
ul.oe_menu div ul li a:hover{
 +
background:#8583bf;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
ul.oe_menu li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
ul.oe_menu2{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu2 > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
 +
}
 +
 +
ul.oe_menu2 > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#eee;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu2 > li > a:hover,
 +
ul.oe_menu2 > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
 +
.oe_wrapper2 ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
 +
ul.oe_menu2 div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu2 div ul li a{
 +
text-decoration:none;
 +
color:#ffffff;
 +
padding:2px 2px 2px 2px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
 +
ul.oe_menu2 div ul.oe_full2{
 +
width:100%;
 +
}
 +
 +
ul.oe_menu2 div ul li a:hover{
 +
background:#d37c62;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
 +
ul.oe_menu2 li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
#main
 +
{
 +
min-height: 100%;
 +
width: 1024px;
 +
margin: 0 auto;
 +
background-color: transparent;
 +
background-image:url("https://static.igem.org/mediawiki/2012/d/da/Kaist_home_background_y_extended.png");
 +
background-repeat:repeat-y;
 +
}
 +
 +
#main #main-img
 +
{
 +
position: absolute;
 +
width: 1024px;
 +
top: -30px;
 +
z-index:0;
 +
}
 +
 +
#main a #main-logo
 +
{
 +
position: absolute;
 +
top: -30px;
 +
width: 336px;
 +
z-index:1;
 +
}
 +
 +
#main #top-img
 +
{
 +
position: relative;
 +
top: -5px;
 +
        left:294px;
 +
width:729px;
 +
height: 250px;
 +
z-index: 0;
 +
opacity:0.9;
 +
}
 +
 +
div .oe_wrapper{
 +
position:absolute;
 +
z-index:3;
 +
top:275px;
 +
}
 +
 +
div .oe_wrapper2{
 +
position:absolute;
 +
z-index:2;
 +
top:358px;
 +
}
 +
 +
div .iGEMLogo{
 +
position:absolute;
 +
z-index:4;
 +
bottom: 80px;
 +
padding : 0 0 0 10px;
 +
}
 +
 +
#menucontent
 +
{
 +
        position: relative;
 +
        background-color:transparent;
 +
        width:690px;
 +
        left:320px;
 +
        top:-75px;
 +
        z-index:2;
 +
        text-align: right;
 +
        opacity:0.8;
 +
}
 +
 +
#top-img-description
 +
{
 +
font-size:25px;
 +
font-family: 'Gloria Hallelujah', cursive;
 +
}
 +
 +
#top-img-description-box
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        top:-115px;
 +
left: 350px;
 +
        z-index:3;
 +
        height:15px;
 +
}
 +
 +
#horizontal-line
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        width:732px;
 +
height:3px;
 +
        left:292px;
 +
        top:-90px;
 +
        z-index:2;
 +
}
 +
div .contact-us{
 +
position: absolute;
 +
z-index:2;
 +
top:450px;
 +
padding:4px 0px 0px 10px;
 +
width:268px;
 +
color:white;
 +
font-weight:bold;
 +
}
 +
 +
div .contact-us #contact-us-information{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
}
 +
 +
div .contact-us #contact-us-information a:hover{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:#fcf967;
 +
font-weight:bold;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-information a{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-title{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:22px;
 +
line-height:170%;
 +
color:#dddddd;
 +
height:30px;
 +
}
 +
 +
</style>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Source+Sans+Pro:300,400,300italic:latin' ] }
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })(); </script>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 +
<meta http-equiv="imagetoolbar" content="no" />
 +
<script>  
var skin="igem",
var skin="igem",
stylepath="/wiki/skins",
stylepath="/wiki/skins",
Line 36: Line 655:
wgCanonicalSpecialPageName=false,
wgCanonicalSpecialPageName=false,
wgNamespaceNumber=0,
wgNamespaceNumber=0,
-
wgPageName="Team:KAIST_Korea",
+
wgPageName="Team:KAIST_Korea/Project",
-
wgTitle="Team:KAIST_Korea",
+
wgTitle="Team:KAIST Korea/Project",
wgAction="view",
wgAction="view",
-
wgArticleId=74,
+
wgArticleId=9665,
wgIsArticle=true,
wgIsArticle=true,
wgUserName="Springkjw",
wgUserName="Springkjw",
Line 46: Line 665:
wgContentLanguage="en",
wgContentLanguage="en",
wgBreakFrames=false,
wgBreakFrames=false,
-
wgCurRevisionId=73131,
+
wgCurRevisionId=297918,
-
wgVersion="1.16.5",
+
wgVersion="1.16.0",
wgEnableAPI=true,
wgEnableAPI=true,
wgEnableWriteAPI=true,
wgEnableWriteAPI=true,
Line 78: Line 697:
         <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
         <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
-
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 
-
<meta name="description" content="" />
 
-
<meta name="keywords" content="" />
 
-
<noscript>
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core.css" />
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-desktop.css" />
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-1200px.css" />
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-noscript.css" />
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/style.css" />
 
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/style-desktop.css" />
 
-
</noscript>
 
-
<script src="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/jquery.js"></script>
 
-
<script src="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/init.js"></script>
 
-
<!--[if IE 9]><link rel="stylesheet" href="css/style-ie9.css" /><![endif]-->
 
-
</head>
 
-
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
 
-
<script type="text/javascript" src="https://dl.dropboxusercontent.com/u/202315040/igem/jquery.easing.1.3.js"></script>
 
-
     
 
-
 
-
<style>
 
-
/* Reset */
 
-
.menu,
 
-
.menu ul,
 
-
.menu li,
 
-
.menu a {
 
-
margin: 0;
 
-
padding: 0;
 
-
border: none;
 
-
outline: none;
 
-
}
 
-
 
-
#bar{
 
-
 
-
background: #4c4e5a;
 
-
background: -webkit-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -moz-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -o-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -ms-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
margin: 30px auto 0 auto;
 
-
 
-
}
 
 +
<link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Open+Sans:600italic,400,800:latin' ]|[ 'Gloria+Hallelujah::latin' ]}
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })();
 +
  </script>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800);
 +
#kaistcontent
#kaistcontent
{
{
Line 128: Line 723:
width:690px;
width:690px;
font-family: 'Open Sans', sans-serif;
font-family: 'Open Sans', sans-serif;
-
font-size:10pt;
+
font-size:11pt;
-
line-height:120%;
+
line-height:140%;
-
text-align:justify;
+
padding: 0px 15px 0px 15px;
padding: 0px 15px 0px 15px;
left:300px;
left:300px;
Line 136: Line 730:
z-index: 2;
z-index: 2;
}
}
-
 
+
-
#kaistcontent #little
+
#kaistcontent #tab
-
{
+
{
-
padding: 0px 30px 0px 30px;
+
width:200px;
-
line-height:140%;
+
height:30px;
-
text-align:justify;
+
position:relative;
-
}
+
background:#f2f3f3;
-
 
+
padding:5px 30px 5px 30px;
 +
border:1px solid #f4f4f4;
 +
margin : 0 20px 0 20px;
 +
}
 +
 +
 +
#kaistcontent #starter-grad
#kaistcontent #starter-grad
{
{
-
width:15px;
+
width:17px;
height:100px;
height:100px;
float:left;
float:left;
margin:0px 15px 2px 0px;
margin:0px 15px 2px 0px;
}
}
-
 
+
#kaistcontent #starter
#kaistcontent #starter
{
{
font-family: 'Open Sans', sans-serif;
font-family: 'Open Sans', sans-serif;
-
font-size:14pt;
+
font-size:25pt;
color: #08185d;
color: #08185d;
 +
font-style:italic
padding:0px 0px 0px 10px;
padding:0px 0px 0px 10px;
}
}
-
 
+
#kaistcontent #sub-title
#kaistcontent #sub-title
{
{
Line 169: Line 770:
font-style:italic;
font-style:italic;
}
}
-
 
+
#kaistcontent h1
#kaistcontent h1
{
{
Line 178: Line 779:
line-height:110%;
line-height:110%;
}
}
-
 
+
#kaistcontent ul
#kaistcontent ul
{
{
list-style-type:disc;
list-style-type:disc;
-
padding: 0px 5px 0px 30px;
+
padding: 0px 0px 0px 30px;
-
 
+
}
}
-
 
+
#kaistcontent li
#kaistcontent li
{
{
-
list-style-type:disc;
+
list-style-type:decimal;
}
}
-
 
+
 +
#kaistcontent a { 
 +
color:#5a5b5e; 
 +
} 
 +
 +
#kaistcontent a:hover{ 
 +
    color:#94969a; 
 +
} 
 +
 +
 +
#kaistcontent #little
 +
{
 +
padding: 0px 0px 0px 30px;
 +
line-height:140%;
 +
}
 +
#kaistcontent #little2
 +
{
 +
padding: 0px 0px 0px 15px;
 +
line-height:140%;
 +
}
 +
#kaistcontent #sub-sub-title
#kaistcontent #sub-sub-title
{
{
Line 199: Line 820:
line-height:110%;
line-height:110%;
}
}
 +
 +
</style>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
 +
        <script type="text/javascript">
 +
            $(function() {
 +
var $oe_menu = $('#oe_menu');
 +
var $oe_menu_items = $oe_menu.children('li');
 +
 +
                $oe_menu_items.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
 +
 +
$oe_menu.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items.children('div').hide();
 +
})
 +
            });
 +
</script>
 +
<script type="text/javascript">
 +
     
 +
$(function() {
 +
var $oe_menu2 = $('#oe_menu2');
 +
var $oe_menu_items2 = $oe_menu2.children('li');
 +
 +
                $oe_menu_items2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items2.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
 +
 +
$oe_menu2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items2.children('div').hide();
 +
})
 +
            });
 +
        </script>
-
/* Menu */
 
-
.menu {
 
-
height: 50px;
 
-
weight: 100%;
 
-
padding-left: 30%;
 
-
+
<style>
-
+
#kaist-footerbox
-
 
+
{
-
background: #4c4e5a;
+
position: relative;
-
background: -webkit-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
background-color: transparent;
-
background: -moz-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
width:1024px;
-
background: -o-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
height:56px;
-
background: -ms-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
z-index:2;
-
background: linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
margin:0 auto;
-
margin: 30px auto 0 auto;
+
bottom:30px;
-
+
}
}
-
 
+
-
.menu li {
+
#kaist-footerbox img #footer-img
-
position: relative;
+
{
-
list-style: none;
+
position: center;
-
margin: 0px auto 0 auto;
+
width: 1024px;
-
float: left;
+
height:106px;
-
display: block;
+
z-index:2;
-
height: 50px;
+
opacity:0.9;
 +
margin:0 auto;
}
}
 +
 +
 +
</style>
 +
</head>
 +
<body>
 +
<div id="main">
-
/* Links */
 
-
.menu li a {
+
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
-
margin:0px auto;
+
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
-
display: block;
+
-
padding: 0 14px;
+
-
margin: 6px 0;
+
-
line-height: 40px;
+
-
text-decoration: none;
+
-
+
-
border-left: 1px solid #393942;
+
-
border-right: 1px solid #4f5058;
+
-
font-family: Helvetica, Arial, sans-serif;
+
-
font-weight: bold;
+
-
font-size: 18px;
+
-
 
+
-
color: #f3f3f3;
+
-
text-shadow: 1px 1px 1px rgba(0,0,0,.6);
+
-
 
+
-
-webkit-transition: color .2s ease-in-out;
+
-
-moz-transition: color .2s ease-in-out;
+
-
-o-transition: color .2s ease-in-out;
+
-
-ms-transition: color .2s ease-in-out;
+
-
transition: color .2s ease-in-out;
+
-
}
+
-
 
+
-
.menu li:first-child a { border-left: none; }
+
-
.menu li:last-child a{ border-right: none; }
+
-
 
+
-
.menu li:hover > a { color: #F29661; }
+
-
 
+
-
/* Sub Menu */
+
-
 
+
-
.menu ul {
+
-
position: absolute;
+
-
top: 50px;
+
-
left: 0px;
+
<div class="oe_wrapper">
-
 
+
<ul id="oe_menu" class="oe_menu">
-
padding-right: 10px;
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
 +
<div style="top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
 +
</ul>
 +
<ul style="width:100px;margin:0px 5px 0px 5px;">
 +
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
 +
<div style="left:-85px;top:25px;"><!-- -112px -->
 +
<ul>
 +
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
 +
<div style="left:-182px;top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="oe_wrapper2">
-
opacity: 0;
+
<ul id="oe_menu2" class="oe_menu2">
-
+
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
-
background: #1f2024;
+
<div style="top:21px;left:32px;">
-
 
+
<ul style="width:100px;height:19px;">
-
-webkit-border-radius: 0 0 5px 5px;
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
-
-moz-border-radius: 0 0 5px 5px;
+
</ul>
-
border-radius: 0 0 5px 5px;
+
<ul style="width:100px;height:19px;">
-
 
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
-
-webkit-transition: opacity .25s ease .1s;
+
</ul>
-
-moz-transition: opacity .25s ease .1s;
+
<ul style="width:100px;height:19px;">
-
-o-transition: opacity .25s ease .1s;
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
-
-ms-transition: opacity .25s ease .1s;
+
</ul>
-
transition: opacity .25s ease .1s;
+
<ul style="width:100px;height:19px;">
-
}
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
-
 
+
</ul>
-
.menu li:hover > ul { opacity: 1; }
+
</div>
-
 
+
</li>
-
.menu ul li {
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
-
height: 0;
+
<div style="left:-91px;top:30px;"><!-- -112px -->
-
overflow: hidden;
+
<ul style="margin:0 0 0 30px;width:100px;">
-
padding: 0px;
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
-
 
+
</ul>
-
-webkit-transition: height .25s ease .1s;
+
<ul style="width:100px">
-
-moz-transition: height .25s ease .1s;
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
-
-o-transition: height .25s ease .1s;
+
</ul>
-
-ms-transition: height .25s ease .1s;
+
-
transition: height .25s ease .1s;
+
</div>
-
}
+
</li>
-
 
+
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
-
.menu li:hover > ul li {
+
<div style="left:-152px;top:21px;">
-
height: 40px;
+
<ul style="width:100px;height:19px;">
-
overflow: visible;
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
-
padding: 0;
+
</ul>
-
}
+
<ul style="width:100px;height:19px;">
-
 
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
-
.menu ul li a {
+
</ul>
-
width: 100px;
+
<ul style="width:100px;height:19px;">
-
padding: 4px 0 4px 40px;
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
-
margin: 0;
+
</ul>
-
 
+
<ul style="width:100px;height:19px;">
-
border: none;
+
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
-
border-bottom: 1px solid #353539;
+
</ul>
-
}
+
</div>
-
 
+
</li>
-
.menu ul li:last-child a { border: none; }
+
-
 
+
-
/* Icons */
+
-
 
+
-
.menu a.documents { background: url(docs.png) no-repeat 6px center; }
+
-
.menu a.messages { background: url(bubble.png) no-repeat 6px center; }
+
-
.menu a.signout { background: url(arrow.png) no-repeat 6px center; }
+
-
 
+
-
 
+
-
ul.menu { margin: 0px auto 0 auto; }
+
-
</style>
+
-
 
+
-
<body class="mediawiki  ltr ns-0 ns-subject page-Team_KIAST_Korea">
+
-
<!-- <header id="header" >  -->
+
-
+
-
<div  id="logo" align="center"> <!-- Logo -->
+
-
+
-
<img height="250" src="https://static.igem.org/mediawiki/2013/thumb/7/70/Logo_kaist.png/800px-Logo_kaist.png" alt=""/>
+
-
+
-
+
-
</div>
+
-
+
-
 
+
-
<!-- menu bar -->
+
-
<div>
+
-
<div id="bar">
+
-
<ul class="menu" >
+
</ul>
-
 
+
</div>
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/index.html">Home</a></li>
+
<div class="contact-us">
-
<li><a href="#">Team</a></li>
+
<div id="contact-us-title">2013 KAIST Korea</div>
-
<li><a href="#">Project</a>
+
<div id="contact-us-information">
 +
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
 +
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
 +
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
 +
</div>
 +
</div>
 +
 +
<div class="iGEMLogo">
 +
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
 +
</div>
 +
</div>
 +
<div id="main">
 +
<ul class="menu">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea">Home</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Team">Team</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">Project</a>
<!-- <ul>
<!-- <ul>
Line 358: Line 1,031:
<li><a href="#">Labnote</a></li>
<li><a href="#">Labnote</a></li>
<li><a href="#">Human Practice</a></li>
<li><a href="#">Human Practice</a></li>
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/safety.html">Safety</a></li>
+
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Safety">Safety</a></li>
</ul> <!-- end .menu -->
</ul> <!-- end .menu -->
-
</div>
 
-
</div>
 
-
      <!-- </header> -->
 
-
 
+
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
-
 
+
-
<body >
+
-
 
+
-
<div id="page-wrapper" class="raw">
+
<div id="kaistcontent">
-
<div class="5grid-layout">
+
-
 
+
</br>
-
 
+
<section id="1st">
-
<!--
+
<div>
-
<div id="feature-content">
+
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
-
<section>
+
<h1>Project : Overview</h1>
-
<h2>RAPTOR</h2>
+
-
</section>
+
</br>
-
 
+
-
 
+
-
<div class="row">
+
-
<div class="12u">
+
-
<div class="row">
+
-
<div class="6u">
+
-
<section id="content">
+
-
<div class="image-style1"><a href="#"><iframe &autoplay=1 width="570" height="315" src="//www.youtube.com/embed/wEwxiU1PP9Y" frameborder="0" allowfullscreen></iframe></a></div>
+
-
                            </section>
+
-
</div>
+
-
<div class="3u">
+
-
<section id = "sidebar">
+
-
<div class="12u">
+
-
<p>KAIST_Korea</p>
+
-
</div>
+
-
<p class="button-style"><a href="#">GO TO ABSTRACT</a></p>
+
-
</section>
+
-
</div>
+
-
</div>
+
-
</div>
+
</div>
</div>
-
</div>
+
-
       
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
-
-->   
+
</br>
-
<div id="page">
+
<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
-
<div class="row">
+
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The <span id="starter">RAPTOR</span> (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
-
<div class="12u">
+
-
<div class="row">
+
-
<div class="8u">
+
-
<section id="content">
+
-
<h2>Introduce My team project RAPTOR.</h2>
+
-
<br><br>
+
-
<p><a href="#"><img src="images/pics02.jpg" alt=""></a></p>
+
-
<p>The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
+
-
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
+
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
-
                                <br><br><br><br><br><br><br><br>  
+
</br></br></span>
-
</section>
+
<div clear='both'></div>
-
</div>
+
-
<!--
+
-
<div class="4u">
+
-
<section id="sidebar">
+
-
<h2>What is CRISPR?</h2>
+
-
<p class="subtitle"></p>
+
</br></br>
-
<p>
+
<div align='right'><a href="#top">▲Back to the top</a></div></br>
-
                                <br><br></p>
+
-
+
-
</section>
+
</section>
-
</div>
+
-
-->
+
-
</div>
+
-
</div>
+
-
</div>
+
-
</div>
+
</div>
</div>
</div>
</div>
-
<!-- PAGE FOOTER -- ITEMS FROM COLUMN ! HAVE BEEN MOVED HERE -- RDR  -->
+
  <div id="kaist-footerbox"><img id="footer-img" align="middle" src="https://static.igem.org/mediawiki/2013/e/ef/Footer_background_kaist.png"></img>
-
<div class="visualClear"></div>
+
-
    <div id='footer-box' class='noprint'>
+
-
        <div id="footer">
+
-
              <div id="f-poweredbyico"><a href="http://www.mediawiki.org/"><img src="/wiki/skins/common/images/poweredby_mediawiki_88x31.png" height="31" width="88" alt="Powered by MediaWiki" /></a></div>       <div id="f-copyrightico"><a href="http://creativecommons.org/licenses/by/3.0/"><img src="http://i.creativecommons.org/l/by/3.0/88x31.png" alt="Attribution 3.0 Unported" width="88" height="31" /></a></div>     <ul id="f-list">
+
-
+
-
 
+
-
  <!-- Recentchanges is not handles well DEBUG -->
+
-
    <li id="t-recentchanges"><a href="/Special:RecentChanges"
+
-
      title='Recent changes'>Recent changes</a></li>
+
-
 
+
-
    <li id="t-whatlinkshere"><a href="/Special:WhatLinksHere/Team:Cornell"
+
-
      title="List of all wiki pages that link here [j]" accesskey="j">What links here</a></li>
+
-
 
+
-
                        <li id="t-recentchangeslinked"><a href="/Special:RecentChangesLinked/Team:Cornell"
+
-
                          title="Recent changes in pages linked from this page [k]" accesskey="k">Related changes</a></li>
+
-
 
+
-
 
+
-
 
+
-
                <li id="t-upload"><a href="/Special:Upload"
+
-
                  title="Upload files [u]" accesskey="u">Upload file</a>
+
-
                </li>
+
-
                <li id="t-specialpages"><a href="/Special:SpecialPages"
+
-
                  title="List of all special pages [q]" accesskey="q">Special pages</a>
+
-
                </li>
+
-
                <li><a href='/Special:Preferences'>My preferences</a></li>
+
-
            </ul>
+
-
        </div> <!-- close footer -->
+
-
        <div id='footer'>
+
-
    <ul id="f-list">
+
-
 
+
-
            <li id="t-print"><a href="/wiki/index.php?title=Team:Cornell&amp;printable=yes"
+
-
              title="Printable version of this page [p]" accesskey="p">Printable version</a>
+
-
            </li>
+
-
 
+
-
            <li id="t-permalink"><a href="/wiki/index.php?title=Team:Cornell&amp;oldid=73131"
+
-
              title="Permanent link to this revision of the page">Permanent link</a>
+
-
            </li>
+
-
 
+
-
 
+
-
        <li id="privacy"><a href="/2013.igem.org:Privacy_policy" title="2013.igem.org:Privacy policy">Privacy policy</a></li>
+
-
        <li id="disclaimer"><a href="/2013.igem.org:General_disclaimer" title="2013.igem.org:General disclaimer">Disclaimers</a></li>
+
-
    </ul>
+
-
</div> <!-- close footer -->
+
-
    </div> <!-- close footer-box -->
+
-
+
-
<script>if (window.runOnloadHook) runOnloadHook();</script>
+
</div>
</div>
-
<!-- Served in 0.158 secs. --></body>
+
</body>
-
 
+
</html>
</html>

Latest revision as of 10:12, 23 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.




Retrieved from "http://2013.igem.org/Team:KAIST_Korea"