Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
 
(83 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
+
<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
 +
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en" dir="ltr">
<head>
<head>
-
<title>iGEM Team : KAIST_Korea</title>
+
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
-
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
+
<meta name="generator" content="MediaWiki 1.16.5" />
-
<meta name="description" content="" />
+
<link rel="alternate" type="application/x-wiki" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<meta name="keywords" content="" />
+
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
-
<noscript>
+
<link rel="shortcut icon" href="/favicon.ico" />
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core.css" />
+
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-desktop.css" />
+
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-1200px.css" />
+
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/core-noscript.css" />
+
<meta name="viewport" content="width=device-width, initial-scale=1.0 minimum-scale=1, maximum-scale=1, user-scalable=yes, target-densitydpi=medium-dpi" />
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/style.css" />
+
<link href='http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic' rel='stylesheet' type='text/css'>
-
<link rel="stylesheet" href="https://dl.dropboxusercontent.com/u/202315040/igem/css/style-desktop.css" />
+
<title>Team:KAIST Korea/Project Background - 2013.igem.org</title>
-
</noscript>
+
<style type="text/css" media="screen, projection">/*<![CDATA[*/
-
+
@import "/wiki/skins/common/shared.css?270";
-
<script src="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/jquery.js"></script>
+
@import "/wiki/skins/igem/main.css?270";
-
<script src="https://dl.dropboxusercontent.com/u/202315040/igem/css/5grid/init.js"></script>
+
#bar{z-index : 5;
-
<!--[if IE 9]><link rel="stylesheet" href="css/style-ie9.css" /><![endif]-->
+
}
-
</head><body>
+
#menu,
-
+
#menu ul,
-
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"></script>
+
#menu li,
-
<script type="text/javascript" src="https://dl.dropboxusercontent.com/u/202315040/igem/jquery.easing.1.3.js"></script>
+
#menu a {
-
     
+
-
+
-
<style>
+
-
/* Reset */
+
-
.menu,
+
-
.menu ul,
+
-
.menu li,
+
-
.menu a {
+
margin: 0;
margin: 0;
-
padding: 0;
+
padding-left: 40;
border: none;
border: none;
outline: none;
outline: none;
}
}
-
 
-
#bar{
 
-
 
-
background: #4c4e5a;
 
-
background: -webkit-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -moz-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -o-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: -ms-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
background: linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
 
-
margin: 30px auto 0 auto;
 
-
 
-
}
 
-
 
-
#kaistcontent
 
-
{
 
-
position: relative;
 
-
background-color:transparent;
 
-
width:690px;
 
-
font-family: 'Open Sans', sans-serif;
 
-
font-size:10pt;
 
-
line-height:120%;
 
-
text-align:justify;
 
-
padding: 0px 15px 0px 15px;
 
-
left:300px;
 
-
top:-70px;
 
-
z-index: 2;
 
-
}
 
-
 
-
#kaistcontent #little
 
-
{
 
-
padding: 0px 30px 0px 30px;
 
-
line-height:140%;
 
-
text-align:justify;
 
-
}
 
-
 
-
#kaistcontent #starter-grad
 
-
{
 
-
width:15px;
 
-
height:100px;
 
-
float:left;
 
-
margin:0px 15px 2px 0px;
 
-
}
 
-
 
-
#kaistcontent #starter
 
-
{
 
-
font-family: 'Open Sans', sans-serif;
 
-
font-size:14pt;
 
-
color: #08185d;
 
-
padding:0px 0px 0px 10px;
 
-
}
 
-
 
-
#kaistcontent #sub-title
 
-
{
 
-
font-family: 'Open Sans', sans-serif;
 
-
color:#848383;
 
-
font-size: 15pt;
 
-
line-height:100%;
 
-
font-weight:bold;
 
-
font-style:italic;
 
-
}
 
-
 
-
#kaistcontent h1
 
-
{
 
-
font-family: 'Open Sans', sans-serif;
 
-
color:#5c5858;
 
-
font-weight:bold;
 
-
font-size:24pt;
 
-
line-height:110%;
 
-
}
 
-
 
-
#kaistcontent ul
 
-
{
 
-
list-style-type:disc;
 
-
padding: 0px 5px 0px 30px;
 
-
 
-
}
 
-
 
-
#kaistcontent li
 
-
{
 
-
list-style-type:disc;
 
-
}
 
-
 
-
#kaistcontent #sub-sub-title
 
-
{
 
-
font-family: 'Open Sans', sans-serif;
 
-
color:#848383;
 
-
font-weight:bold;
 
-
font-size:14pt;
 
-
line-height:110%;
 
-
}
 
-
 
-
/* Menu */
 
.menu {
.menu {
height: 50px;
height: 50px;
weight: 100%;
weight: 100%;
padding-left: 30%;
padding-left: 30%;
-
+
        z-index: 2;
-
+
background: #000000;
-
+
-
+
-
background: #4c4e5a;
+
-
background: -webkit-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -moz-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -o-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -ms-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
margin: 30px auto 0 auto;
+
-
+
}
}
   
   
Line 160: Line 51:
padding: 0 14px;
padding: 0 14px;
margin: 6px 0;
margin: 6px 0;
-
line-height: 40px;
+
text-decoration: none;
text-decoration: none;
-
border-left: 1px solid #393942;
+
 
-
border-right: 1px solid #4f5058;
+
   
   
font-family: Helvetica, Arial, sans-serif;
font-family: Helvetica, Arial, sans-serif;
font-weight: bold;
font-weight: bold;
-
font-size: 18px;
+
font-size: 20px;
   
   
-
color: #f3f3f3;
+
color: #353535;
-
text-shadow: 1px 1px 1px rgba(0,0,0,.6);
+
   
   
-webkit-transition: color .2s ease-in-out;
-webkit-transition: color .2s ease-in-out;
Line 250: Line 140:
ul.menu { margin: 0px auto 0 auto; }
ul.menu { margin: 0px auto 0 auto; }
</style>
</style>
 +
 +
<style type="text/css">
 +
 +
/* Cross-Browsing */
 +
html,body,div,ul,ol,li,dl,dt,dd,h1,h2,h3,h4,h5,h6,pre,form,p,blockquote,fieldset,input,hr {margin:0; padding:0;}
 +
h1,h2,h3,h4,h5,h6,pre,code,address,caption,cite,code,em,strong,th {font-size:1em; font-weight:normal; font-style:normal;}
 +
ol, ul, li { list-style:none; }
 +
fieldset,img,hr {border:none;}
 +
caption,th {text-align:left;}
 +
table {border-collapse:collapse; border-spacing:0;}
 +
td {vertical-align:top;}
 +
strong { font-weight:bold ;}
 +
em { font-style:italic; }
 +
a img { border:none; }
 +
q:before, q:after { content:''}
   
   
 +
html { height: 100%; }
 +
 +
 +
body
 +
{
 +
background-image: url('https://static.igem.org/mediawiki/2012/6/61/Top_section_expand_KAIST.png'),url('https://static.igem.org/mediawiki/2012/9/98/PATTERN_diagonal.png');
 +
background-repeat: repeat-x,repeat;
 +
margin: 0;
 +
padding: 0;
 +
}
 +
   
   
-
<!-- logo -->
+
-
+
#content
-
<!-- <header id="header" >  -->
+
{
-
+
border: none;
-
<div  id="logo" align="center"> <!-- Logo -->
+
position: relative;
 +
top: 0;
 +
background-color: transparent;
 +
padding: 0;
 +
width: 100%;
 +
}
 +
 +
#bodyContent p
 +
{
 +
display: none;
 +
}
 +
 +
#globalWrapper
 +
{
 +
clear: both;
 +
width: 100%;
 +
                z-index: 1;
 +
position: relative;
 +
top:0px;
 +
margin: auto;
 +
font-size: 10px;
 +
}
 +
 +
#contentSub { display: none; }
 +
#siteSub { display:none; }
 +
 +
#search-controls {
 +
left: 20px;
 +
width: auto;
 +
        z-index: 100;
 +
        position: relative;
 +
        float: right;
 +
        top: -10px;
 +
        right: 0;
 +
        height: auto;
 +
        clear: both;
 +
}
 +
 +
input.searchButton
 +
{
 +
position: relative;
 +
font-size: 1em;
 +
font-family: "Arial";
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 +
#searchInput
 +
{
 +
background-color: transparent;
 +
z-index: 100;
 +
color: #181818;
 +
font: Arial;
 +
text-align: center;
-
<img height="250"  src="https://static.igem.org/mediawiki/2013/thumb/7/70/Logo_kaist.png/800px-Logo_kaist.png" alt=""/>
+
background: url('https://static.igem.org/mediawiki/2012/6/6e/Kaist2012_head_searchbar.png') no-repeat;
 +
background-position: 0px 0px;
 +
vertical-align: middle;
 +
width: 184px;
 +
height: 22px;
 +
border: none;
 +
}
 +
 +
#searchGoButton
 +
{
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/7/78/Kaist2012_head_arrow.png') no-repeat;
 +
background-position: left 0px;
 +
        z-index: 100;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
#mw-searchButton
 +
{
 +
position: relative;
 +
left: -14px;
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
 +
                z-index: 100;
 +
background-position: left 0px;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
.firstHeading { display:none; }
 +
 +
#p-logo
 +
{
 +
display: none;
 +
position: relative;
 +
top: 0px;
 +
z-index: 0;
 +
height: 1px;
 +
width: 10px;
 +
overflow: hidden;
 +
}
 +
 +
#p-logo a { color: black; }
 +
 +
img[src*="/wiki/skins/common/images/wiki.png"]
 +
{
 +
    visibility: hidden;
 +
    top: -100px;
 +
    width: 10px;
 +
    height: 0px;
 +
margin-top: 20px;
 +
    background: transparent;
 +
}
 +
 +
 +
 +
#menubar
 +
{
 +
font-size: 10px;
 +
font-family: "GothamXNarrow-Bold", "Helvetica";
 +
position: relative;
 +
float: left;
 +
width: auto;
 +
top: 36px;
 +
        z-index: 100;
 +
}
-
</div>
+
#menubar:hover{ background-color: #181818; }
-
+
 +
.left-menu { margin-top: 0px; left: 0; }
 +
.left-menu ul { border: none;}
 +
.right-menu { margin-top: 0px; right: 0; float: right !important;}
 +
.right-menu ul { border: none; }
 +
.right-menu ul a { background-color: transparent; }
 +
.left-menu li a { color: #615d5a; }
 +
.left-menu li a:visited { color: #615d5a; }
 +
.left-menu li a:hover { color: #ffffff; }
 +
.left-menu li a:active{ color: #cccccc; font-weight: bold; }
 +
.right-menu li a { color: #f6f5c8; }
 +
.right-menu li a:visited { color: #f6f5c8; }
 +
.right-menu li a:hover { color: #ffffff; text-decoration: none; }
 +
.right-menu li a:active { color: #cccccc; text-decoration: none;font-weight: bold; }
 +
 +
#menubar li {
   
   
-
<!-- menu bar -->
+
        margin: 0px;
-
<div>
+
        word-spacing:-1px;
-
<div id="bar">
+
}
-
<ul class="menu" >
+
#catlinks {
 +
position: relative;
 +
top: 5px;
 +
left: -5px;
 +
width: 1034px;
 +
border: none;
 +
background-color: transparent;
 +
padding-top: 5px;
 +
clear: both;
 +
}
 +
 +
#footer-box {display:none;}
 +
#footer {display:none;}
 +
#footer li a { color: #000000; }
 +
#footer img { margin: 10px; border: none; }
 +
#footer li a:visited {color: #000000;}
 +
#footer li a:hover { color: #ababab; text-decoration: none; }
 +
#footer li a:active{ color: #ababab; text-decoration: none; font-weight: bold}
 +
        </style>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Source+Sans+Pro:300,400,300italic);
 +
ul.oe_menu{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
   
   
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/index.html">Home</a></li>
+
}
-
<li><a href="#">Team</a></li>
+
-
<li><a href="#">Project</a>
+
   
   
 +
ul.oe_menu > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#aaa;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu > li > a:hover,
 +
ul.oe_menu > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
.oe_wrapper ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
ul.oe_menu div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu div ul li a{
 +
text-decoration:none;
 +
color:#cbcbcb;
 +
padding:2px 2px 2px 4px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
ul.oe_menu div ul.oe_full{
 +
width:100%;
 +
}
 +
ul.oe_menu div ul li a:hover{
 +
background:#8583bf;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
ul.oe_menu li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
ul.oe_menu2{
 +
list-style:none;
 +
position:relative;
 +
width:300px;
 +
float:left;
 +
clear:both;
 +
margin:5px 0px 0px 8px;
 +
}
 +
ul.oe_menu2 > li{
 +
width:90px;
 +
height:30px;
 +
padding:auto;
 +
float:left;
 +
position:relative;
 +
list-style-type:none;
 +
}
 +
 +
ul.oe_menu2 > li > a{
 +
display:block;
 +
background-color:transparent;
 +
color:#eee;
 +
text-decoration:none;
 +
text-align:center;
 +
font-size:16px;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
width:90px;
 +
height:30px;
 +
opacity:0.8;
 +
}
 +
ul.oe_menu2 > li > a:hover,
 +
ul.oe_menu2 > li.selected > a{
 +
color:#fff;
 +
opacity:1.0;
 +
}
 +
 +
.oe_wrapper2 ul.hovered > li > a{
 +
background:transparent;
 +
 +
}
 +
 +
ul.oe_menu2 div{
 +
position:absolute;
 +
left:0px;
 +
background:transparent;
 +
width:268px;
 +
height:36px;
 +
padding:2px;
 +
display:none;
 +
}
 +
ul.oe_menu2 div ul li a{
 +
text-decoration:none;
 +
color:#ffffff;
 +
padding:2px 2px 2px 2px;
 +
display:block;
 +
font-family: 'Source Sans Pro', sans-serif;
 +
text-align:center;
 +
font-size:16px;
 +
}
 +
 +
ul.oe_menu2 div ul.oe_full2{
 +
width:100%;
 +
}
 +
 +
ul.oe_menu2 div ul li a:hover{
 +
background:#d37c62;/*이걸로 색상 조절*/
 +
color:#fff;
 +
}
 +
 +
ul.oe_menu2 li ul{
 +
list-style:none;
 +
float:left;
 +
width: 86px;
 +
margin-right:0px;
 +
}
 +
 +
#main
 +
{
 +
min-height: 100%;
 +
width: 1024px;
 +
margin: 0 auto;
 +
background-color: transparent;
 +
background-image:url("https://static.igem.org/mediawiki/2012/d/da/Kaist_home_background_y_extended.png");
 +
background-repeat:repeat-y;
 +
}
 +
 +
#main #main-img
 +
{
 +
position: absolute;
 +
width: 1024px;
 +
top: -30px;
 +
z-index:0;
 +
}
 +
 +
#main a #main-logo
 +
{
 +
position: absolute;
 +
top: -30px;
 +
width: 336px;
 +
z-index:1;
 +
}
 +
 +
#main #top-img
 +
{
 +
position: relative;
 +
top: -5px;
 +
        left:294px;
 +
width:729px;
 +
height: 250px;
 +
z-index: 0;
 +
opacity:0.9;
 +
}
 +
 +
div .oe_wrapper{
 +
position:absolute;
 +
z-index:3;
 +
top:275px;
 +
}
 +
 +
div .oe_wrapper2{
 +
position:absolute;
 +
z-index:2;
 +
top:358px;
 +
}
 +
 +
div .iGEMLogo{
 +
position:absolute;
 +
z-index:4;
 +
bottom: 80px;
 +
padding : 0 0 0 10px;
 +
}
 +
 +
#menucontent
 +
{
 +
        position: relative;
 +
        background-color:transparent;
 +
        width:690px;
 +
        left:320px;
 +
        top:-75px;
 +
        z-index:2;
 +
        text-align: right;
 +
        opacity:0.8;
 +
}
 +
 +
#top-img-description
 +
{
 +
font-size:25px;
 +
font-family: 'Gloria Hallelujah', cursive;
 +
}
 +
 +
#top-img-description-box
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        top:-115px;
 +
left: 350px;
 +
        z-index:3;
 +
        height:15px;
 +
}
 +
 +
#horizontal-line
 +
{
 +
position: relative;
 +
        background-color:transparent;
 +
        width:732px;
 +
height:3px;
 +
        left:292px;
 +
        top:-90px;
 +
        z-index:2;
 +
}
 +
div .contact-us{
 +
position: absolute;
 +
z-index:2;
 +
top:450px;
 +
padding:4px 0px 0px 10px;
 +
width:268px;
 +
color:white;
 +
font-weight:bold;
 +
}
 +
 +
div .contact-us #contact-us-information{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
}
 +
 +
div .contact-us #contact-us-information a:hover{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:#fcf967;
 +
font-weight:bold;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-information a{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:12px;
 +
font-style: italic;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
 +
div .contact-us #contact-us-title{
 +
font-family: 'Source Sans Pro', sans-serif;
 +
font-size:22px;
 +
line-height:170%;
 +
color:#dddddd;
 +
height:30px;
 +
}
 +
 +
</style>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Source+Sans+Pro:300,400,300italic:latin' ] }
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })(); </script>
 +
<link rel="stylesheet" type="text/css" media="print" href="/wiki/skins/common/commonPrint.css?270" />
 +
<meta http-equiv="imagetoolbar" content="no" />
 +
<script>
 +
var skin="igem",
 +
stylepath="/wiki/skins",
 +
wgUrlProtocols="http\\:\\/\\/|https\\:\\/\\/|ftp\\:\\/\\/|irc\\:\\/\\/|gopher\\:\\/\\/|telnet\\:\\/\\/|nntp\\:\\/\\/|worldwind\\:\\/\\/|mailto\\:|news\\:|svn\\:\\/\\/",
 +
wgArticlePath="/$1",
 +
wgScriptPath="/wiki",
 +
wgScriptExtension=".php",
 +
wgScript="/wiki/index.php",
 +
wgVariantArticlePath=false,
 +
wgActionPaths={},
 +
wgServer="https://2013.igem.org",
 +
wgCanonicalNamespace="",
 +
wgCanonicalSpecialPageName=false,
 +
wgNamespaceNumber=0,
 +
wgPageName="Team:KAIST_Korea/Project",
 +
wgTitle="Team:KAIST Korea/Project",
 +
wgAction="view",
 +
wgArticleId=9665,
 +
wgIsArticle=true,
 +
wgUserName="Springkjw",
 +
wgUserGroups=["*", "user", "autoconfirmed"],
 +
wgUserLanguage="en",
 +
wgContentLanguage="en",
 +
wgBreakFrames=false,
 +
wgCurRevisionId=297918,
 +
wgVersion="1.16.0",
 +
wgEnableAPI=true,
 +
wgEnableWriteAPI=true,
 +
wgSeparatorTransformTable=["", ""],
 +
wgDigitTransformTable=["", ""],
 +
wgMainPageTitle="Main Page",
 +
wgFormattedNamespaces={"-2": "Media", "-1": "Special", "0": "", "1": "Talk", "2": "User", "3": "User talk", "4": "2013.igem.org", "5": "2013.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10": "Template", "11": "Template talk", "12": "Help", "13": "Help talk", "14": "Category", "15": "Category talk"},
 +
wgNamespaceIds={"media": -2, "special": -1, "": 0, "talk": 1, "user": 2, "user_talk": 3, "2013.igem.org": 4, "2013.igem.org_talk": 5, "file": 6, "file_talk": 7, "mediawiki": 8, "mediawiki_talk": 9, "template": 10, "template_talk": 11, "help": 12, "help_talk": 13, "category": 14, "category_talk": 15, "image": 6, "image_talk": 7},
 +
wgSiteName="2013.igem.org",
 +
wgCategories=[],
 +
wgMWSuggestTemplate="https://2013.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
 +
wgDBname="2013_igem_org",
 +
wgSearchNamespaces=[0],
 +
wgMWSuggestMessages=["with suggestions", "no suggestions"],
 +
wgRestrictionEdit=[],
 +
wgRestrictionMove=[],
 +
wgAjaxWatch={"watchMsg": "Watch", "unwatchMsg": "Unwatch", "watchingMsg": "Watching...", "unwatchingMsg": "Unwatching...", "tooltip-ca-watchMsg": "Add this page to your watchlist", "tooltip-ca-unwatchMsg": "Remove this page from your watchlist"};
 +
</script>               
 +
<script type="text/javascript" src="/wiki/skins/common/wikibits.js?270"><!-- wikibits js --></script>
 +
<!-- Head Scripts -->
 +
<script src="/wiki/skins/common/ajax.js?270"></script>
 +
<script src="/wiki/skins/common/ajaxwatch.js?270"></script>
 +
<script src="/wiki/skins/common/mwsuggest.js?270"></script>
 +
<script type="text/javascript" src="/wiki/index.php?title=-&amp;action=raw&amp;smaxage=0&amp;gen=js&amp;useskin=igem"><!-- site js --></script>
 +
<!-- jQuery Javascript -->
 +
<script type='text/javascript'        src ='/common/jquery-latest.min.js'></script>
 +
<script type='text/javascript'        src ='/common/tablesorter/jquery.tablesorter.min.js'></script>
 +
        <link rel='stylesheet' type='text/css' href='/common/tablesorter/themes/groupparts/style.css' />
 +
        <link rel='stylesheet' type='text/css' href='/common/table_styles.css' />
 +
        <link rel='stylesheet' type='text/css' href='/forum/forum_styles.css' />
 +
<script type='text/javascript'        src ='/forum/forum_scripts.js'></script>
 +
 +
 +
<link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
 +
<script type="text/javascript">
 +
  WebFontConfig = {
 +
    google: { families: [ 'Open+Sans:600italic,400,800:latin' ]|[ 'Gloria+Hallelujah::latin' ]}
 +
  };
 +
  (function() {
 +
    var wf = document.createElement('script');
 +
    wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
 +
      '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
 +
    wf.type = 'text/javascript';
 +
    wf.async = 'true';
 +
    var s = document.getElementsByTagName('script')[0];
 +
    s.parentNode.insertBefore(wf, s);
 +
  })();
 +
  </script>
 +
<style>
 +
@import url(http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800);
 +
 +
#kaistcontent
 +
{
 +
position: relative;
 +
background-color:transparent;
 +
width:690px;
 +
font-family: 'Open Sans', sans-serif;
 +
font-size:11pt;
 +
line-height:140%;
 +
padding: 0px 15px 0px 15px;
 +
left:300px;
 +
top:-70px;
 +
z-index: 2;
 +
}
 +
 +
#kaistcontent #tab
 +
{
 +
width:200px;
 +
height:30px;
 +
position:relative;
 +
background:#f2f3f3;
 +
padding:5px 30px 5px 30px;
 +
border:1px solid #f4f4f4;
 +
margin : 0 20px 0 20px;
 +
}
 +
 +
 +
 +
#kaistcontent #starter-grad
 +
{
 +
width:17px;
 +
height:100px;
 +
float:left;
 +
margin:0px 15px 2px 0px;
 +
}
 +
 +
#kaistcontent #starter
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
font-size:25pt;
 +
color: #08185d;
 +
font-style:italic
 +
padding:0px 0px 0px 10px;
 +
}
 +
 +
#kaistcontent #sub-title
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#848383;
 +
font-size: 15pt;
 +
line-height:100%;
 +
font-weight:bold;
 +
font-style:italic;
 +
}
 +
 +
#kaistcontent h1
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#5c5858;
 +
font-weight:bold;
 +
font-size:24pt;
 +
line-height:110%;
 +
}
 +
 +
#kaistcontent ul
 +
{
 +
list-style-type:disc;
 +
padding: 0px 0px 0px 30px;
 +
 +
}
 +
 +
#kaistcontent li
 +
{
 +
list-style-type:decimal;
 +
}
 +
 +
#kaistcontent a { 
 +
color:#5a5b5e; 
 +
 +
 +
#kaistcontent a:hover{ 
 +
    color:#94969a; 
 +
 +
 +
 +
#kaistcontent #little
 +
{
 +
padding: 0px 0px 0px 30px;
 +
line-height:140%;
 +
}
 +
#kaistcontent #little2
 +
{
 +
padding: 0px 0px 0px 15px;
 +
line-height:140%;
 +
}
 +
 +
#kaistcontent #sub-sub-title
 +
{
 +
font-family: 'Open Sans', sans-serif;
 +
color:#848383;
 +
font-weight:bold;
 +
font-size:14pt;
 +
line-height:110%;
 +
}
 +
 +
</style>
 +
<script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.3/jquery.min.js"></script>
 +
        <script type="text/javascript">
 +
            $(function() {
 +
var $oe_menu = $('#oe_menu');
 +
var $oe_menu_items = $oe_menu.children('li');
 +
 +
                $oe_menu_items.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
 +
 +
$oe_menu.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items.children('div').hide();
 +
})
 +
            });
 +
</script>
 +
<script type="text/javascript">
 +
     
 +
$(function() {
 +
var $oe_menu2 = $('#oe_menu2');
 +
var $oe_menu_items2 = $oe_menu2.children('li');
 +
 +
                $oe_menu_items2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('slided selected');
 +
$this.children('div').css('z-index','9999').stop(true,true).slideDown(200,function(){
 +
$oe_menu_items2.not('.slided').children('div').hide();
 +
$this.removeClass('slided');
 +
});
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('selected').children('div').css('z-index','1');
 +
});
 +
 +
$oe_menu2.bind('mouseenter',function(){
 +
var $this = $(this);
 +
$this.addClass('hovered');
 +
}).bind('mouseleave',function(){
 +
var $this = $(this);
 +
$this.removeClass('hovered');
 +
$oe_menu_items2.children('div').hide();
 +
})
 +
            });
 +
        </script>
 +
 +
 +
<style>
 +
#kaist-footerbox
 +
{
 +
position: relative;
 +
background-color: transparent;
 +
width:1024px;
 +
height:56px;
 +
z-index:2;
 +
margin:0 auto;
 +
bottom:30px;
 +
}
 +
 +
#kaist-footerbox img #footer-img
 +
{
 +
position: center;
 +
width: 1024px;
 +
height:106px;
 +
z-index:2;
 +
opacity:0.9;
 +
margin:0 auto;
 +
}
 +
 +
 +
</style>
 +
</head>
 +
<body>
 +
<div id="main">
 +
 +
 +
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
 +
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
 +
 +
 +
 +
<div class="oe_wrapper">
 +
<ul id="oe_menu" class="oe_menu">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">HOME</a>
 +
<div style="top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Home_Intro">Introduction</a></li>
 +
</ul>
 +
<ul style="width:100px;margin:0px 5px 0px 5px;">
 +
<li style="font-size:14px;"><a href="https://2012.igem.org/Team:KAIST_Korea/Home_iGEM">iGEM</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Members">TEAM</a>
 +
<div style="left:-85px;top:25px;"><!-- -112px -->
 +
<ul>
 +
<li><a style="font-size:14px;"href="https://igem.org/Team.cgi?id=907">Official Team Profile</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_KAIST">Members</a></li>
 +
</ul>
 +
<ul style="padding:5px 0px 5px 0px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Team_Gallery">Gallery</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">DATA</a>
 +
<div style="left:-182px;top:30px;">
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_About">Description</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Part_Bio">Bio Bricks</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="oe_wrapper2">
 +
 +
<ul id="oe_menu2" class="oe_menu2">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
 +
<div style="top:21px;left:32px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Background">Overview</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Results">Results</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Future">Future Plan</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Project_Modeling">Modeling</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">NOTEBOOK</a>
 +
<div style="left:-91px;top:30px;"><!-- -112px -->
 +
<ul style="margin:0 0 0 30px;width:100px;">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote">Labnote</a></li>
 +
</ul>
 +
<ul style="width:100px">
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol">Protocol</a></li>
 +
</ul>
 +
 +
</div>
 +
</li>
 +
<li><a href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">PLUS</a>
 +
<div style="left:-152px;top:21px;">
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Safety">Safety</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Human">Human Practice</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Attribution">Attribution</a></li>
 +
</ul>
 +
<ul style="width:100px;height:19px;">
 +
<li><a style="font-size:14px;" href="https://2012.igem.org/Team:KAIST_Korea/Plus_Sponsor">Sponsors</a></li>
 +
</ul>
 +
</div>
 +
</li>
 +
 +
</ul>
 +
</div>
 +
<div class="contact-us">
 +
<div id="contact-us-title">2013 KAIST Korea</div>
 +
<div id="contact-us-information">
 +
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
 +
<b>Twitter</b> : <a href="http://twitter.com/KAIST_iGEM_2012">twitter.com/KAIST_iGEM_2012</a></br>
 +
<b>Facebook</b> : <a href="http://www.facebook.com/KAISTiGEM2012">www.facebook.com/KAISTiGEM2012</a>
 +
</div>
 +
</div>
 +
 +
<div class="iGEMLogo">
 +
<a href="https://igem.org/Main_Page"><img src="https://static.igem.org/mediawiki/2012/0/00/KAIST_iGEM_logo.png"/></a>
 +
</div>
 +
</div>
 +
<div id="main">
 +
<ul class="menu">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea">Home</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Team">Team</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">Project</a>
 +
<!-- <ul>
<!-- <ul>
<li><a href="#" class="documents">Documents</a></li>
<li><a href="#" class="documents">Documents</a></li>
Line 281: Line 1,027:
-->
-->
-
+
 
</li>
</li>
<li><a href="#">Labnote</a></li>
<li><a href="#">Labnote</a></li>
<li><a href="#">Human Practice</a></li>
<li><a href="#">Human Practice</a></li>
-
<li><a href="https://dl.dropboxusercontent.com/u/202315040/igem/safety.html">Safety</a></li>
+
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Safety">Safety</a></li>
-
+
 
</ul> <!-- end .menu -->
</ul> <!-- end .menu -->
-
</div>
+
 
-
</div>
+
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
-
      <!-- </header> -->
+
   
   
 +
 +
 +
<div id="kaistcontent">
   
   
 +
</br>
 +
<section id="1st">
 +
<div>
 +
<img id="starter-grad" style="height:60px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
 +
<h1>Project : Overview</h1>
 +
 +
</br>
 +
</div>
   
   
-
<body >
+
<img src="https://static.igem.org/mediawiki/2012/e/e1/KAIST_lionLion.png" style="float:left;padding:10px 20px 10px 0px;margin:0px 0px 20px 0px;"/>
 +
</br>
 +
<span id="little"><span id="starter">T</span>he widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
 +
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The <span id="starter">RAPTOR</span> (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
 +
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
 +
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
 +
</br></br></span>
 +
<div clear='both'></div>
 +
 +
 +
 +
 +
 +
</br></br>
 +
<div align='right'><a href="#top">▲Back to the top</a></div></br>
   
   
-
<div id="page-wrapper" class="raw">
 
-
<div class="5grid-layout">
 
   
   
-
 
-
<!--
 
-
<div id="feature-content">
 
-
<section>
 
-
<h2>RAPTOR</h2>
 
</section>
</section>
   
   
   
   
-
<div class="row">
 
-
<div class="12u">
 
-
<div class="row">
 
-
<div class="6u">
 
-
<section id="content">
 
-
<div class="image-style1"><a href="#"><iframe &autoplay=1 width="570" height="315" src="//www.youtube.com/embed/wEwxiU1PP9Y" frameborder="0" allowfullscreen></iframe></a></div>
 
-
                            </section>
 
-
</div>
 
-
<div class="3u">
 
-
<section id = "sidebar">
 
-
<div class="12u">
 
-
<p>KAIST_Korea</p>
 
-
</div>
 
-
<p class="button-style"><a href="#">GO TO ABSTRACT</a></p>
 
-
</section>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
       
 
-
-->   
 
-
<div id="page">
 
-
<div class="row">
 
-
<div class="12u">
 
-
<div class="row">
 
-
<div class="8u">
 
-
<section id="content">
 
-
<h2>Introduce My team project RAPTOR.</h2>
 
-
<br><br>
 
-
<p></p>
 
-
<p>The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation.
 
-
iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.
 
-
Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.
 
-
As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.
 
-
                                <br><br><br><br><br><br><br><br>
 
-
</section>
 
-
</div>
 
-
<!--
 
-
<div class="4u">
 
-
<section id="sidebar">
 
-
<h2>What is CRISPR?</h2>
 
-
<p class="subtitle"></p>
 
-
<p>
 
-
                                <br><br></p>
 
-
 
-
</section>
 
-
</div>
 
-
-->
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</div>
 
</div>
</div>
 +
</div>
 +
<div id="kaist-footerbox"><img id="footer-img" align="middle" src="https://static.igem.org/mediawiki/2013/e/ef/Footer_background_kaist.png"></img>
</div>
</div>
</body>
</body>
-
<!--<div id="footer-wrapper">
 
-
<div id="footer-content" class="5grid-layout">
 
-
<div class="row">
 
-
 
-
<div class="3u">
 
-
 
-
</div>
 
-
<div class="6u">
 
-
<section>
 
-
<h2>footer site</h2>
 
-
<p>iGEM logo </p>
 
-
 
-
</section>
 
-
</div>
 
-
</div>
 
-
</div>
 
-
</div> -->
 
-
<div class="5grid-layout">
 
-
<div id="copyright">
 
-
<section>
 
-
<p>&copy; Your Site Name | Images: <a href="http://fotogrph.com/">Fotogrph</a> | Design: <a href="http://html5templates.com/">HTML5Templates.com</a></p>
 
-
</section>
 
-
</div>
 
-
</div>
 
-
 
</html>
</html>

Latest revision as of 10:12, 23 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.