Team:KAIST Korea

From 2013.igem.org

(Difference between revisions)
 
(36 intermediate revisions not shown)
Line 7: Line 7:
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
<link rel="edit" title="Edit" href="/wiki/index.php?title=Team:KAIST_Korea/Project&amp;action=edit" />
<link rel="shortcut icon" href="/favicon.ico" />
<link rel="shortcut icon" href="/favicon.ico" />
-
 
+
<link rel="search" type="application/opensearchdescription+xml" href="/wiki/opensearch_desc.php" title="2013.igem.org (en)" />
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
<link title="Creative Commons" type="application/rdf+xml" href="/wiki/index.php?title=Team:KAIST_Korea/Project_Background&amp;action=creativecommons" rel="meta" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
<link rel="copyright" href="http://creativecommons.org/licenses/by/3.0/" />
Line 16: Line 16:
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/common/shared.css?270";
@import "/wiki/skins/igem/main.css?270";
@import "/wiki/skins/igem/main.css?270";
-
 
+
#bar{z-index : 5;
-
.menu,
+
}
-
.menu ul,
+
#menu,
-
.menu li,
+
#menu ul,
-
.menu a {
+
#menu li,
 +
#menu a {
margin: 0;
margin: 0;
-
padding: 0;
+
padding-left: 40;
border: none;
border: none;
outline: none;
outline: none;
Line 30: Line 31:
weight: 100%;
weight: 100%;
padding-left: 30%;
padding-left: 30%;
-
+
        z-index: 2;
-
+
background: #000000;
-
+
-
+
-
background: #4c4e5a;
+
-
background: -webkit-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -moz-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -o-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: -ms-linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
background: linear-gradient(top, #4c4e5a 0%,#2c2d33 100%);
+
-
margin: 30px auto 0 auto;
+
-
+
}
}
   
   
Line 60: Line 51:
padding: 0 14px;
padding: 0 14px;
margin: 6px 0;
margin: 6px 0;
-
line-height: 40px;
+
text-decoration: none;
text-decoration: none;
-
border-left: 1px solid #393942;
+
 
-
border-right: 1px solid #4f5058;
+
   
   
font-family: Helvetica, Arial, sans-serif;
font-family: Helvetica, Arial, sans-serif;
font-weight: bold;
font-weight: bold;
-
font-size: 18px;
+
font-size: 20px;
   
   
-
color: #f3f3f3;
+
color: #353535;
-
text-shadow: 1px 1px 1px rgba(0,0,0,.6);
+
   
   
-webkit-transition: color .2s ease-in-out;
-webkit-transition: color .2s ease-in-out;
Line 171: Line 161:
body
body
{
{
-
background-image: url('https://static.igem.org/mediawiki/2012/9/98/PATTERN_diagonal.png');
+
background-image: url('https://static.igem.org/mediawiki/2012/6/61/Top_section_expand_KAIST.png'),url('https://static.igem.org/mediawiki/2012/9/98/PATTERN_diagonal.png');
-
+
background-repeat: repeat-x,repeat;
margin: 0;
margin: 0;
padding: 0;
padding: 0;
}  
}  
 +
   
   
Line 197: Line 188:
clear: both;
clear: both;
width: 100%;
width: 100%;
-
        z-index: 1;
+
                z-index: 1;
position: relative;
position: relative;
top:0px;
top:0px;
Line 207: Line 198:
#siteSub { display:none; }
#siteSub { display:none; }
 +
#search-controls {
 +
left: 20px;
 +
width: auto;
 +
        z-index: 100;
 +
        position: relative;
 +
        float: right;
 +
        top: -10px;
 +
        right: 0;
 +
        height: auto;
 +
        clear: both;
 +
}
 +
 +
input.searchButton
 +
{
 +
position: relative;
 +
font-size: 1em;
 +
font-family: "Arial";
 +
font-weight: normal;
 +
color: #FFFFFF;
 +
}
 +
 +
#searchInput
 +
{
 +
background-color: transparent;
 +
z-index: 100;
 +
color: #181818;
 +
font: Arial;
 +
text-align: center;
 +
 +
background: url('https://static.igem.org/mediawiki/2012/6/6e/Kaist2012_head_searchbar.png') no-repeat;
 +
background-position: 0px 0px;
 +
vertical-align: middle;
 +
 +
width: 184px;
 +
height: 22px;
 +
border: none;
 +
}
 +
 +
#searchGoButton
 +
{
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/7/78/Kaist2012_head_arrow.png') no-repeat;
 +
background-position: left 0px;
 +
        z-index: 100;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
 +
 +
#mw-searchButton
 +
{
 +
position: relative;
 +
left: -14px;
 +
font-size: 0pt;
 +
background: url('https://static.igem.org/mediawiki/2012/e/e1/Kaist2012_head_search.png') no-repeat;
 +
                z-index: 100;
 +
background-position: left 0px;
 +
cursor: pointer;
 +
width: 29px;
 +
height: 29px;
 +
border: none;
 +
}
.firstHeading { display:none; }
.firstHeading { display:none; }
Line 465: Line 519:
position: relative;
position: relative;
top: -5px;
top: -5px;
-
    left:292px;
+
        left:294px;
-
width:732px;
+
width:729px;
height: 250px;
height: 250px;
z-index: 0;
z-index: 0;
Line 622: Line 676:
wgSiteName="2013.igem.org",
wgSiteName="2013.igem.org",
wgCategories=[],
wgCategories=[],
 +
wgMWSuggestTemplate="https://2013.igem.org/wiki/api.php?action=opensearch\x26search={searchTerms}\x26namespace={namespaces}\x26suggest",
wgDBname="2013_igem_org",
wgDBname="2013_igem_org",
 +
wgSearchNamespaces=[0],
wgMWSuggestMessages=["with suggestions", "no suggestions"],
wgMWSuggestMessages=["with suggestions", "no suggestions"],
wgRestrictionEdit=[],
wgRestrictionEdit=[],
Line 851: Line 907:
<body>
<body>
<div id="main">
<div id="main">
 +
 +
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
<a href="https://2013.igem.org/Team:KAIST_Korea"><img id="main-logo" src="https://static.igem.org/mediawiki/2013/5/59/Logopart_Kaist.png"/></a>
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
<img id="main-img" src="https://static.igem.org/mediawiki/2012/6/61/Home_kaist_background.png"/>
 +
 +
 +
<div class="oe_wrapper">
<div class="oe_wrapper">
<ul id="oe_menu" class="oe_menu">
<ul id="oe_menu" class="oe_menu">
Line 892: Line 953:
</div>
</div>
<div class="oe_wrapper2">
<div class="oe_wrapper2">
 +
<ul id="oe_menu2" class="oe_menu2">
<ul id="oe_menu2" class="oe_menu2">
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">PROJECT</a>
Line 940: Line 1,002:
</div>
</div>
<div class="contact-us">
<div class="contact-us">
-
<div id="contact-us-title">2
+
<div id="contact-us-title">2013 KAIST Korea</div>
-
 
+
-
 
+
-
 
+
-
013 KAIST Korea</div>
+
<div id="contact-us-information">
<div id="contact-us-information">
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
<b>Mail</b> : <a href="mailto:kaist.igem.2012@gmail.com">kaist.igem.2012@gmail.com</a></br>
Line 957: Line 1,015:
</div>
</div>
<div id="main">
<div id="main">
 +
<ul class="menu">
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea">Home</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Team">Team</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Project">Project</a>
 +
 +
<!-- <ul>
 +
<li><a href="#" class="documents">Documents</a></li>
 +
<li><a href="#" class="messages">Messages</a></li>
 +
<li><a href="#" class="signout">Sign Out</a></li>
 +
</ul>
 +
 +
-->
 +
 +
</li>
 +
<li><a href="#">Labnote</a></li>
 +
<li><a href="#">Human Practice</a></li>
 +
<li><a href="https://2013.igem.org/Team:KAIST_Korea/Safety">Safety</a></li>
 +
 +
</ul> <!-- end .menu -->
 +
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
<img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
   
   

Latest revision as of 10:12, 23 August 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> Team:KAIST Korea/Project Background - 2013.igem.org


Project : Overview



The widely used gene knock-out strategies are based upon a mere [on-off] control and more complex regulation is impossible. On the other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because of the intricate design and scar accumulation. iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes. Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control. As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.