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Team:KAIST Korea/Project - Revision history
2024-03-28T19:36:40Z
Revision history for this page on the wiki
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Springkjw at 07:22, 22 August 2013
2013-08-22T07:22:04Z
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Springkjw
http://2013.igem.org/wiki/index.php?title=Team:KAIST_Korea/Project&diff=81990&oldid=prev
Springkjw at 20:35, 21 August 2013
2013-08-21T20:35:57Z
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Springkjw
http://2013.igem.org/wiki/index.php?title=Team:KAIST_Korea/Project&diff=81977&oldid=prev
Springkjw at 20:30, 21 August 2013
2013-08-21T20:30:08Z
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Springkjw
http://2013.igem.org/wiki/index.php?title=Team:KAIST_Korea/Project&diff=63922&oldid=prev
Ethan1028: /* Overall project */
2013-08-09T14:51:05Z
<p><span class="autocomment">Overall project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Tell us </del>more <del class="diffchange diffchange-inline">about your project. Give us background. Use this </del>is the <del class="diffchange diffchange-inline">abstract </del>of <del class="diffchange diffchange-inline">your project</del>. <del class="diffchange diffchange-inline"> Be descriptive but concise (1-2 paragraphs)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The widely used gene knock-out strategies are based upon a mere ‘on-off’ control and </ins>more <ins class="diffchange diffchange-inline">complex regulation </ins>is <ins class="diffchange diffchange-inline">impossible. On </ins>the <ins class="diffchange diffchange-inline">other hand, knock-down strategies are more versatile in usage but the current techniques are vastly laborious for systematic control and pose difficulty in multiple targeting because </ins>of <ins class="diffchange diffchange-inline">the intricate design and scar accumulation</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">iGEM KAIST 2013 seeks to take the yet-to-be-perfected genetic regulation technology to a new level: The RAPTOR (RNA Associated Prokaryotic Transcript Optimization with CRISPR) technology. RAPTOR uses CRISPR type III system for gene regulation. CRISPR type III targets and cleaves specific mRNA sequences through complementary binding of crRNA and nuclease activity of Cmr protein complex. RAPTOR exploits this nature of CRISPR for systematically down regulating the mRNA level of specific genes.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Due to the characteristics of RAPTOR, one of the most useful applications of this technology can be biosynthesis pathway regulation. Programmed crRNA expression allows fine-tuning of target mRNA expression levels and can precisely regulate enzyme concentrations. Not only fine-tuning but multiplex engineering is also possible through simultaneous expression of different crRNAs. Thus, multiple enzymes can be targeted in pathway control.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">As a proof of concept we sought to regulate the lycopene pathway that is incorporated into the genome of Escherichia coli. The efflux of lycopene precursors is optimally reduced through lowering the mRNA levels of enzymes that synthesize non-lycopene end products. Through observation of increased lycopene yield in the E.coli, potentiality of RAPTOR technology can be validated.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td></tr>
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Ethan1028
http://2013.igem.org/wiki/index.php?title=Team:KAIST_Korea/Project&diff=2920&oldid=prev
IGEM HQ: Prototype team page
2013-04-26T02:56:12Z
<p>Prototype team page</p>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:KAIST_Korea|Home]]<br />
!align="center"|[[Team:KAIST_Korea/Team|Team]]<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=KAIST_Korea Official Team Profile]<br />
!align="center"|[[Team:KAIST_Korea/Project|Project]]<br />
!align="center"|[[Team:KAIST_Korea/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:KAIST_Korea/Modeling|Modeling]]<br />
!align="center"|[[Team:KAIST_Korea/Notebook|Notebook]]<br />
!align="center"|[[Team:KAIST_Korea/Safety|Safety]]<br />
!align="center"|[[Team:KAIST_Korea/Attributions|Attributions]]<br />
|}<br />
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== '''Overall project''' ==<br />
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== Project Details==<br />
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=== Part 2 ===<br />
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=== The Experiments ===<br />
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=== Part 3 ===<br />
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== Results ==</div>
IGEM HQ