Team:KU Leuven/Project/StickerSystem

From 2013.igem.org

(Difference between revisions)
 
(16 intermediate revisions not shown)
Line 4: Line 4:
<html>
<html>
-
 
<body>
<body>
<div id="container">
<div id="container">
<div class="container">
<div class="container">
 +
 +
<!--LOGOS-->
 +
 +
<div class="row-fluid">
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">
 +
  <div class="visible-desktop span3 bg-grey1">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/Aphid_Background">
 +
    <i class="livicon activeicon" data-name="bug" data-color="white"></i></div>
 +
  <div class="span7 icon-text">
 +
    <h3>Aphid Background</h3>
 +
    </a>
 +
    <p>Crashcourse in aphid biology</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
   
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">
 +
  <div class="visible-desktop span3 bg-grey1">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/HoneydewSystem">
 +
    <i class="livicon activeicon" data-name="drop" data-color="white"></i></div>
 +
  <div class="span7 icon-text">
 +
    <h3>Honeydew System</h3> </a>
 +
    <p>Our BanAphids react to honeydew</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">
 +
  <div class="visible-desktop span3 bg-green">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem">
 +
    <i class="livicon activeicon" data-name="refresh" data-color="white"></i>
 +
    </div>
 +
  <div class="span7 icon-text">
 +
    <h3>Sticker System</h3>
 +
    </a>
 +
    <p>You are here!</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
</div>
 +
 +
 +
<div class="row-fluid">
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">
 +
  <div class="visible-desktop span3 bg-grey1">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/E.coligy">
 +
    <i class="livicon activeicon" data-name="leaf" data-color="white"></i>
 +
  </div>
 +
  <div class="span7 icon-text">
 +
    <h3><i>E. coligy</i></h3>
 +
    </a>
 +
    <p>Validating the BanAphids in vivo</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">
 +
  <div class="visible-desktop span3 bg-grey1">
 +
  <a href="https://2013.igem.org/Team:KU_Leuven/Parts">
 +
    <i class="livicon activeicon" data-name="gears" data-color="white"></i></div>
 +
  <div class="span7 icon-text">
 +
    <h3>Parts</h3> </a>
 +
    <p>BioBrick 'm all!</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
<div class="span4 icon white">
 +
  <div class="row-fluid">   
 +
    <div class="visible-desktop span3 bg-grey1">
 +
    <a href="https://2013.igem.org/Team:KU_Leuven/Project/DataPage">
 +
    <i class="livicon activeicon" data-name="info" data-color="white"></i>
 +
    </div>
 +
  <div class="span7 icon-text">
 +
    <h3>Data Page</h3> </a>
 +
    <p>All our achievements on one small page!</p>
 +
  </div>
 +
  </div>
 +
</div>
 +
 +
  </div>
 +
 +
<!-- TITLE -->
<!-- TITLE -->
Line 22: Line 112:
  <div class="span12 white">
  <div class="span12 white">
   <p align = "justify">
   <p align = "justify">
-
Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our <b>BanAphids</b> could end up in the environment. <b>We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches</b>. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. <b>Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator.</b> Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.</p>
+
Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our <b>BanAphids</b> could end up in the environment. <b>We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches</b>. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. <b>Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator.</b> The oscillator is extensively elaborated in the <b><a href="https://2013.igem.org/Team:KU_Leuven/Project/Modelling/Colony_Level">oscillator model section</a></b>. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.</p>
  </div>
  </div>
</div>
</div>
-
<!-- Design -->
+
<!-- Aim -->
<div class="row-fluid">
<div class="row-fluid">
  <div class="span12 icon-small white">
  <div class="span12 icon-small white">
   <div class="row-fluid">
   <div class="row-fluid">
-
   <div class="span2 bg-background visible-desktop">
+
   <div class="span2 bg-farnesene visible-desktop">
<a href = "https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem/Design">
<a href = "https://2013.igem.org/Team:KU_Leuven/Project/StickerSystem/Design">
     <i class="livicon activeicon" data-name="pencil" data-onparent="true" data-color="white"></i>
     <i class="livicon activeicon" data-name="pencil" data-onparent="true" data-color="white"></i>
Line 39: Line 129:
     <div class="row-fluid">
     <div class="row-fluid">
     <div class="span12">
     <div class="span12">
-
       <p align="justify">In this part we describe the design of an oscillator that could be useful in biological networks. We created a system that creates synchronized oscillations without depending heavily on the components used. We explain several necessities to obtain a synchronized oscillator, and how we managed to incorporate those within our network. </p>
+
       <p align="justify">In this part we explain the necessity of the sticker system, as well as its practical execution, such as the properties of the sticker and the implementation of an oscillator. </p>
     </div>
     </div>
     </div>
     </div>
Line 52: Line 142:
  <div class="span12 icon-small white">
  <div class="span12 icon-small white">
   <div class="row-fluid">
   <div class="row-fluid">
-
   <div class="span2 bg-oscillator visible-desktop">
+
   <div class="span2 bg-background visible-desktop">
<a href="https://2013.igem.org/Team:KU_Leuven/Project/Oscillator/wetlab">
<a href="https://2013.igem.org/Team:KU_Leuven/Project/Oscillator/wetlab">
     <i class="livicon activeicon" data-name="lab" data-onparent="true" data-color="white"></i>
     <i class="livicon activeicon" data-name="lab" data-onparent="true" data-color="white"></i>
Line 58: Line 148:
   <div class="span10 icon-text ">
   <div class="span10 icon-text ">
-
     <h3>Wetlab</h3> </a>
+
     <h3>Integrating the oscillator into the wetlab</h3> </a>
     <div class="row-fluid">
     <div class="row-fluid">
     <div class="span12">
     <div class="span12">

Latest revision as of 03:19, 29 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Aphid Background

Crashcourse in aphid biology

Honeydew System

Our BanAphids react to honeydew

Sticker System

You are here!

E. coligy

Validating the BanAphids in vivo

Parts

BioBrick 'm all!

Data Page

All our achievements on one small page!

Our initial system for the production of methyl salicylate and β-farnesene relied on direct interaction between the bacteria and the aphids, our BanAphids would be sprayed on the plant. This is ethically and practically challenging since our BanAphids could end up in the environment. We therefore designed an alternative pheromone production system, where the bacteria are kept in semi-permeable pouches. These allow the pheromones to disperse in the air yet the bacteria themselves remain in the bags. Consequently, the presence of aphids secreting honeydew cannot be used as a trigger for the production of both pheromones. Since constitutive production of β-farnesene rapidly renders aphids insensitive, concentrations should fluctuate to prevent habituation. This is typically achieved in an oscillator. The oscillator is extensively elaborated in the oscillator model section. Here we will mainly focus on the design of a β-farnesene oscillator, a part of it will be actually made in the lab. The production of methyl salicylate can be regulated similarly.

Design

In this part we explain the necessity of the sticker system, as well as its practical execution, such as the properties of the sticker and the implementation of an oscillator.

Integrating the oscillator into the wetlab

A part of the oscillator was implemented in the wetlab. You can find the genes we chose for our oscillator here, as well as the results of the wetlab experiments.