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Team:Korea U Seoul/hp/safty Q
From 2013.igem.org
Would any of your project ideas raise safety issues in terms of:
- Issues to the researcher safety
- E.coli(BW25113, DH5a, BL21(DE3) Moreover, E. coli strain we are using are BW25113, DH5a and BL21(DE3). Those E. colis are used for produce proteins(nacrein) and they use different promoters. Refer to former research they studied widely about safety and by the result founded to be very safe. They also weakened for laboratory use. Therefore would not pose a threat to environment. Thus, this E. coli strain is less likely to harm researchers.
- Vectors Vectors We used is pATLIC and pBL plasmid. They used to produce protein which we want to made by using other promoters. pATLIC and pBL vector have same role but protein which made by pBL system is soluble but protein made by pATLIC is insoluble.
- Risks to the safety and health of the general public There are no significant risks to the safety and health of the general public. First, we do not use pathogenic bacteria. Second, gene products do not induce immune response. Third, our lab follows rules of bio-safety level limiting reagents and material we use. Here are reasons why our project is less likely to cause any health risks.
In conclusion, we find our project harmless. Although no significant safety issues were raised during the experiment, it is advised that experimenter should follow safety protocols listed on this website. This includes wearing gloves and goggles as bacteria may cause irritation and skin rash. Please refer to lab safety protocol: http://kusafe.korea.ac.kr/main.asp (university guideline, Korean) http://bric.postech.ac.kr/myboard/read.php?Board=safety&id=1481(national guideline, Korean)
This plasmid have resist genes against antibiotics. So it can be danger to nature and human. however to transcript DNA and make protein, they need some key materials. So in nature they can be activated. Also we follow safety protocols about definite by our university. Its include biohazard treatment so we can isolate our vector from nature and human.
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? how did you manage to handle the safety issue? How could other teams learn from your experience?
BioBrick we made is Carbonic Anhydrase from nacreous layer in oyster pearls. It can hold CO2 by attach that by CaCO3 form. So CO2 fixed by nacrein have special crystal structure because of nacrein between the CaCO3. Nacrein only action as a Carbonic Anhydrase and it is one of the most universal enzyme. One difference is using Ca to fix CO2. But CaCO3 also popular chemical, So there's no risk for nature.
Also it is hard to express in other species because of the vector which we use need special material to active.
