Team:Kyoto/Notebook

From 2013.igem.org

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====Transformation====
====Transformation====
====Electrophoresis====
====Electrophoresis====

Revision as of 03:50, 21 August 2013

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Material and method Safety Attributions

""m&m""

Contents

RNA Oscillator

Turing Pattern

Aug 20

Miniprep

DNAconcentration[μg/μL]260/280260/230
8/18 Ptet -12201.691.87
8/18 Ptet -22101.691.71
8/18 RBS-tetR-DT -12361.631.69
8/18 RBS-tetR-DT -21821.651.98
8/18 J23100-RBS-GFP-DT -13341.712.12
8/18 J23100-RBS-luxR-DT -14401.671.60
8/18 J23100-RBS-luxR-DT -23441.681.83
8/18 J23100-RBS-lacZα-DT -12801.701.81
8/18 RBS-lysis3 -12821.671.76
8/18 RBS-lysis3 -22121.301.35

LB Culture Medium

volume - 200mL

nameamount
Bacto Trypton2g
Bacto yeast extract1g
Nacl1g
Agar Powder2g
Amp40μl

1.Measured reagents and put they in the Erlenmeyer flask
2.
3.autoclaving


not written yet

Ristriction Enzyme Proccessing

8/20 Ptet -1(220μg/mL)EcoRIXbaI10x BSA10x buffer MMilliQtotal
4.5113317.530
0.50.20117.310
0.500.2117.310
0.500117.510
8/20 RBS-tetR-DT -2(182μg/mL)EcoRISpeI10x buffer HMilliQtotal
5.511319.530
0.60.2018.210
0.600.218.210
0.60018.410

not written yet

Transformation

Electrophoresis