"
Team:Kyoto/Notebook
From 2013.igem.org
(Difference between revisions)
(→LB Culture Medium) |
(→Miniprep) |
||
| Line 28: | Line 28: | ||
====Miniprep==== | ====Miniprep==== | ||
{|class="wikitable" | {|class="wikitable" | ||
| - | + | !DNA!!concentration[μg/μL]!!260/280!!260/230 | |
|- | |- | ||
|8/18 Ptet -1||220||1.69||1.87 | |8/18 Ptet -1||220||1.69||1.87 | ||
Revision as of 05:46, 21 August 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Material and method | Safety | Attributions |
|---|
Contents |
RNA Oscillator
Turing Pattern
Aug 20
Miniprep
| DNA | concentration[μg/μL] | 260/280 | 260/230 |
|---|---|---|---|
| 8/18 Ptet -1 | 220 | 1.69 | 1.87 |
| 8/18 Ptet -2 | 210 | 1.69 | 1.71 |
| 8/18 RBS-tetR-DT -1 | 236 | 1.63 | 1.69 |
| 8/18 RBS-tetR-DT -2 | 182 | 1.65 | 1.98 |
| 8/18 J23100-RBS-GFP-DT -1 | 334 | 1.71 | 2.12 |
| 8/18 J23100-RBS-luxR-DT -1 | 440 | 1.67 | 1.60 |
| 8/18 J23100-RBS-luxR-DT -2 | 344 | 1.68 | 1.83 |
| 8/18 J23100-RBS-lacZα-DT -1 | 280 | 1.70 | 1.81 |
| 8/18 RBS-lysis3 -1 | 282 | 1.67 | 1.76 |
| 8/18 RBS-lysis3 -2 | 212 | 1.30 | 1.35 |
LB Culture Medium
by Okazaki
volume - 200mL
| name | amount |
| Bacto Trypton | 2g |
| Bacto yeast extract | 1g |
| Nacl | 1g |
| Agar Powder | 2g |
| Amp | 40μl |
1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml
not written yet
Ristriction Enzyme Proccessing
by Kojima and Ashida
| 8/20 Ptet -1(220μg/mL) | EcoRI | XbaI | 10x BSA | 10x buffer M | MilliQ | total |
| 4.5 | 1 | 1 | 3 | 3 | 17.5 | 30 |
| 0.5 | 0.2 | 0 | 1 | 1 | 7.3 | 10 |
| 0.5 | 0 | 0.2 | 1 | 1 | 7.3 | 10 |
| 0.5 | 0 | 0 | 1 | 1 | 7.5 | 10 |
| 8/20 RBS-tetR-DT -2(182μg/mL) | EcoRI | SpeI | 10x buffer H | MilliQ | total |
| 5.5 | 1 | 1 | 3 | 19.5 | 30 |
| 0.6 | 0.2 | 0 | 1 | 8.2 | 10 |
| 0.6 | 0 | 0.2 | 1 | 8.2 | 10 |
| 0.6 | 0 | 0 | 1 | 8.4 | 10 |
| 8/20 J23100-RBS-GFP-DT(334μg/ml) | EcoRI | SpeI | 10x buffer H | MilliQ | total |
| 3.0 | 1 | 1 | 3 | 22 | 30 |
| 0.3 | 0.2 | 0 | 1 | 8.5 | 10 |
| 0.3 | 0 | 0.2 | 1 | 8.5 | 10 |
| 0.3 | 0 | 0 | 1 | 8.7 | 10 |
| 8/20 J23100-RBS-luxR-DT -2(344μg/mL) | EcoRI | XbaI | BSA | 10x buffer M | MilliQ | total |
| 2.9 | 1 | 1 | 3 | 3 | 19.1 | 30 |
| 0.3 | 0.2 | 0 | 1 | 1 | 7.5 | 10 |
| 0.3 | 0 | 0.2 | 1 | 1 | 7.5 | 10 |
| 0.3 | 0 | 0 | 1 | 1 | 7.7 | 10 |
| 8/20 RBS-lysis3 -1(282μg/mL) | EcoRI | SpeI | 10x buffer H | MilliQ | total |
| 3.5 | 1 | 1 | 3 | 21.5 | 30 |
| 0.4 | 0.2 | 0 | 1 | 8.4 | 10 |
| 0.4 | 0 | 0.2 | 1 | 8.4 | 10 |
| 0.4 | 0 | 0 | 1 | 8.6 | 10 |
| 8/17 DT -1(188μg/mL) | EcoRI | XbaI | 10x BSA | 10x buffer M | MilliQ | total |
| 5.3 | 1 | 1 | 3 | 3 | 16.7 | 30 |
| 0.3 | 0.2 | 0 | 1 | 1 | 7.5 | 10 |
| 0.3 | 0 | 0.2 | 1 | 1 | 7.5 | 10 |
| 0.3 | 0 | 0 | 1 | 1 | 7.7 | 10 |
incubate ~20:00
