Revision as of 05:48, 21 August 2013

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RNA Oscillator

DNA	concentration[μg/μL]	260/280	260/230
8/18 Ptet -1	220	1.69	1.87
8/18 Ptet -2	210	1.69	1.71
8/18 RBS-tetR-DT -1	236	1.63	1.69
8/18 RBS-tetR-DT -2	182	1.65	1.98
8/18 J23100-RBS-GFP-DT -1	334	1.71	2.12
8/18 J23100-RBS-luxR-DT -1	440	1.67	1.60
8/18 J23100-RBS-luxR-DT -2	344	1.68	1.83
8/18 J23100-RBS-lacZα-DT -1	280	1.70	1.81
8/18 RBS-lysis3 -1	282	1.67	1.76
8/18 RBS-lysis3 -2	212	1.30	1.35

by Okazaki
volume - 200mL

1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml

not written yet

by Kojima and Ashida

8/20 Ptet -1(220μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
4.5	1	1	3	3	17.5	30
0.5	0.2	0	1	1	7.3	10
0.5	0	0.2	1	1	7.3	10
0.5	0	0	1	1	7.5	10

8/20 RBS-tetR-DT -2(182μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
5.5	1	1	3	19.5	30
0.6	0.2	0	1	8.2	10
0.6	0	0.2	1	8.2	10
0.6	0	0	1	8.4	10

8/20 J23100-RBS-GFP-DT(334μg/ml)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.0	1	1	3	22	30
0.3	0.2	0	1	8.5	10
0.3	0	0.2	1	8.5	10
0.3	0	0	1	8.7	10

8/20 J23100-RBS-luxR-DT -2(344μg/mL)	EcoRI	XbaI	BSA	10x buffer M	MilliQ	total
2.9	1	1	3	3	19.1	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

8/20 RBS-lysis3 -1(282μg/mL)	EcoRI	SpeI	10x buffer H	MilliQ	total
3.5	1	1	3	21.5	30
0.4	0.2	0	1	8.4	10
0.4	0	0.2	1	8.4	10
0.4	0	0	1	8.6	10

8/17 DT -1(188μg/mL)	EcoRI	XbaI	10x BSA	10x buffer M	MilliQ	total
5.3	1	1	3	3	16.7	30
0.3	0.2	0	1	1	7.5	10
0.3	0	0.2	1	1	7.5	10
0.3	0	0	1	1	7.7	10

incubate ~20:00

@@ Line 77: / Line 77: @@
 <small>by Kojima and Ashida</small><br>
 {|class="wikitable"
-|8/20 Ptet -1(220μg/mL)||EcoRI||XbaI||10x BSA||10x buffer M||MilliQ||total
+!8/20 Ptet -1(220μg/mL)!!EcoRI!!XbaI!!10x BSA!!10x buffer M!!MilliQ!!total
 |-
 |4.5||1||1||3||3||17.5||30
@@ Line 88: / Line 88: @@
 |}
 {|class="wikitable"
-|8/20 RBS-tetR-DT -2(182μg/mL)||EcoRI||SpeI||10x buffer H||MilliQ||total
+!8/20 RBS-tetR-DT -2(182μg/mL)!!EcoRI!!SpeI!!10x buffer H!!MilliQ!!total
 |-
 |5.5||1||1||3||19.5||30
@@ Line 99: / Line 99: @@
 |}
 {|class="wikitable"
-|8/20 J23100-RBS-GFP-DT(334μg/ml)||EcoRI||SpeI||10x buffer H||MilliQ||total
+!8/20 J23100-RBS-GFP-DT(334μg/ml)!!EcoRI!!SpeI!!10x buffer H!!MilliQ!!total
 |-
 |3.0||1||1||3||22||30
@@ Line 110: / Line 110: @@
 |}
 {|class="wikitable"
-|8/20 J23100-RBS-luxR-DT -2(344μg/mL)||EcoRI||XbaI||BSA||10x buffer M||MilliQ||total
+!8/20 J23100-RBS-luxR-DT -2(344μg/mL)!!EcoRI!!XbaI!!BSA!!10x buffer M!!MilliQ!!total
 |-
 |2.9||1||1||3||3||19.1||30
@@ Line 121: / Line 121: @@
 |}
 {|class="wikitable"
-|8/20 RBS-lysis3 -1(282μg/mL)||EcoRI||SpeI||10x buffer H||MilliQ||total
+!8/20 RBS-lysis3 -1(282μg/mL)!!EcoRI!!SpeI!!10x buffer H!!MilliQ!!total
 |-
 |3.5||1||1||3||21.5||30
@@ Line 132: / Line 132: @@
 |}
 {|class="wikitable"
-|8/17 DT -1(188μg/mL)||EcoRI||XbaI||10x BSA||10x buffer M||MilliQ||total
+!8/17 DT -1(188μg/mL)!!EcoRI!!XbaI!!10x BSA!!10x buffer M!!MilliQ!!total
 |-
 |5.3||1||1||3||3||16.7||30