Team:Kyoto/Notebook

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Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Material and method Safety Attributions

""m&m""

Contents

RNA Oscillator

Turing Pattern

Aug 20

Miniprep

DNAconcentration[μg/μL]260/280260/230
8/18 Ptet -12201.691.87
8/18 Ptet -22101.691.71
8/18 RBS-tetR-DT -12361.631.69
8/18 RBS-tetR-DT -21821.651.98
8/18 J23100-RBS-GFP-DT -13341.712.12
8/18 J23100-RBS-luxR-DT -14401.671.60
8/18 J23100-RBS-luxR-DT -23441.681.83
8/18 J23100-RBS-lacZα-DT -12801.701.81
8/18 RBS-lysis3 -12821.671.76
8/18 RBS-lysis3 -22121.301.35

LB Culture Medium

volume - 200mL

nameamount
Bacto Trypton2g
Bacto yeast extract1g
Nacl1g
Agar Powder2g
Amp40μl

1.Measured reagents and put they in the Erlenmeyer flask
2.
3.autoclaving


not written yet

Ristriction Enzyme Proccessing

Transformation

Electrophoresis