Team:Kyoto/Project

From 2013.igem.org

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= '''Overall project''' =
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<!-- Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs) -->
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<html>
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<a href="https://2013.igem.org/Kyoto:projectRNA">
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  <div>
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<div class="RNALink">
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    <a href="https://2013.igem.org/Team:Kyoto/ProjectTuring">
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Project-RNA
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      <img src="https://static.igem.org/mediawiki/2013/4/47/Turingtop.png" style="padding-bottom:5px;">
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    </a>
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<a href="https://2013.igem.org/Kyoto:projectTuring">
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    <a href="https://2013.igem.org/Team:Kyoto/projectRNA" >
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Project-Turing
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      <img src="https://static.igem.org/mediawiki/2013/4/44/RNAOsjafjsi.png">
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== RNA Oscillator==
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=== motivation ===
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=== Introduction ===
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<html><center><iframe src="http://prezi.com/embed/eaubz-cct4kd/?bgcolor=ffffff&amp;lock_to_path=0&amp;autoplay=0&amp;autohide_ctrls=0&amp;features=undefined&amp;disabled_features=undefined" width="550" height="400" frameBorder="0"></iframe></center></html>
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説明文
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=== Experiments & Results ===
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==== tetR aptamer ====
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tetR aptamerはP_tetにくっついているtetRを外してP_tetの下流の転写を促進する。これを確認したい。<br>
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(図があった方がいいかも、くっついてるtetRにtetR aptamerが向かって行って外れて、RNAPが飛んでくるような？)
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そこで、その確認のために以下のような実験を行った。
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experimental group:<br>
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Pcon-RBS-tetR-DT Ptet-RBS-LacZ-DT Pcon-tetRaptamer-DT
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negative control:<br>
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Pcon-RBS-tetR-DT Ptet-RBS-LacZ-DT
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Result fig
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また、同時にtetRの確認実験として以下のようなものを行った。
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Experimental group:<br>
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Ptet-RBS-GFP-DT Pcon-RBS-tetR-DT
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negative control:<br>
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Ptet-RBS-GFP-DT
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Result fig
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=== attenuator ===
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== Turing Pattern ==
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=== Introduction ===
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=== Experiments ===
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=== Results ===
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Latest revision as of 12:44, 10 October 2013

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