Team:Leeds
From 2013.igem.org
m |
m |
||
Line 7: | Line 7: | ||
</html> | </html> | ||
__NOTOC__ | __NOTOC__ | ||
- | {{Team:Leeds_layout|HeaderParky=</div>[[File:Leeds_ParkyPanoram.png|150px|link=|frameless]]<div>|HeaderImage=[[File:Leeds_HomepageHead.png|825px|frameless]]|Header=Welcome to the Leeds Wiki!|content= | + | {{Team:Leeds_layout|HeaderParky=</div>[[File:Leeds_ParkyPanoram.png|150px|link=|frameless]]<div>|HeaderImage=[[File:Leeds_HomepageHead.png|825px|link=|frameless]]|Header=Welcome to the Leeds Wiki!|content= |
[[File:Leeds sooperheader3.png|center|700px|awesome looking header|link=|frameless]] | [[File:Leeds sooperheader3.png|center|700px|awesome looking header|link=|frameless]] | ||
<br> | <br> |
Latest revision as of 03:52, 5 October 2013
A biological system designed to detect the presence of pathogens in water samplesMicro-Beagle is a novel reporter system for E-coli that, as an iGEM first, has been designed to dynamically detect arbitrary target solids (including other cells) through a mechanism activated by cell surface binding. Micro-Beagle was initially developed to address the need for a low cost, energy efficient and robust tool to be used to assess the effectiveness of current water purification systems. As the Micro-Beagle is intended to operate at a low cost it is ideal for use in low-income areas where the testing of water purification techniques cannot be maintained at an acceptable frequency. Micro-Beagle is designed to also be a modular system, utilising Ice Nucleation Protein to express and position target-binding peptides on the cell surface. Target binding induces membrane stress that activates the Cpx signalling pathway, and Micro-Beagle thus utilises a promoter from this pathway (pCpxR) to initiate expression of a reporter protein, such as GFP. As a proof of concept, we have used silica beads as a model diagnostic target (a pathogen surrogate) and the silica-binding “Si4” sequence as the target-binding peptide. We foresee Micro-Beagle being adapted for both the detection of waterborne pathogens and a variety of other diagnostic applications, and we envision future multisensor Micro-Beagles in which diverse pathogens can be simultaneously and quantitatively measured from a single water sample.
The Leeds 2013 iGEM teamWe are the Leeds 2013 iGEM team, a group of undergrads from various courses at the University of Leeds, all with a passion for synthetic biology!
| |||||||
| |||||||