http://2013.igem.org/wiki/index.php?title=Team:Lethbridge&feed=atom&action=historyTeam:Lethbridge - Revision history2024-03-29T08:55:24ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=240829&oldid=prevJennafriedt at 03:35, 28 September 20132013-09-28T03:35:01Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Project Overview</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Project Overview</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"><del class="diffchange diffchange-inline">The current growth in synthetic biology research promises more complex and useful engineered systems. However</del>, <del class="diffchange diffchange-inline">increased complexity often requires more genetic material that can be difficult to introduce into organisms. The 2013 </del>Lethbridge iGEM <del class="diffchange diffchange-inline">FRAMEchanger project has produced </del>a new class of <del class="diffchange diffchange-inline">gene regulatory </del>parts <del class="diffchange diffchange-inline">that can be used for compression of multiple coding sequences into a smaller amount of genetic space. This is achieved by </del>programmed ribosomal frameshifting <del class="diffchange diffchange-inline">induced by the RNA structural motif called a pseudoknot</del>. <del class="diffchange diffchange-inline">Addtionally, </del>this <del class="diffchange diffchange-inline">part can by used for variable tagging of proteins</del>, <del class="diffchange diffchange-inline">regulated expression of proteins in an operon, and other applications in the field of synthetic biology. The pseudoknot’s ability to induce ribosomal frameshifting can be modulated by changing the stability of </del>the pseudoknot <del class="diffchange diffchange-inline">structure. In this way, a library of frameshifting parts with variable frameshifting frequencies can be made available </del>for <del class="diffchange diffchange-inline">a variety of applications</del>. <del class="diffchange diffchange-inline">A software tool has also been developed </del>to <del class="diffchange diffchange-inline">facilitate zipping multiple coding sequences into overlapped reading </del>frames<del class="diffchange diffchange-inline">. Ultimately, this library </del>of <del class="diffchange diffchange-inline">standardized parts will be available for use in a variety of engineered systems requiring minimal coding space and multiple protein expression</del>.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"><ins class="diffchange diffchange-inline">This year</ins>, <ins class="diffchange diffchange-inline">the </ins>Lethbridge iGEM <ins class="diffchange diffchange-inline">team is working to bring </ins>a <ins class="diffchange diffchange-inline">whole </ins>new class of parts <ins class="diffchange diffchange-inline">to the iGEM community: </ins>programmed ribosomal frameshifting <ins class="diffchange diffchange-inline">elements</ins>. <ins class="diffchange diffchange-inline">To do </ins>this, <ins class="diffchange diffchange-inline">we have been working towards standardizing </ins>the <ins class="diffchange diffchange-inline">PK401 </ins>pseudoknot for <ins class="diffchange diffchange-inline">use within the BioBrick system</ins>. <ins class="diffchange diffchange-inline">These RNA secondary structural elements cause the ribosome </ins>to <ins class="diffchange diffchange-inline">switch between translational </ins>frames <ins class="diffchange diffchange-inline">and give another degree </ins>of <ins class="diffchange diffchange-inline">freedom when engineering genetic circuits</ins>.</p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHAT?</u></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHAT?</u></b></div></td></tr>
</table>Jennafriedthttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=240193&oldid=prevJennafriedt at 03:27, 28 September 20132013-09-28T03:27:08Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Project Overview</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Project Overview</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black">The current growth in synthetic biology research promises more complex and useful engineered systems. However, increased complexity often requires more genetic material that can be difficult to introduce into organisms. <del class="diffchange diffchange-inline">We propose the development of </del>a new <del class="diffchange diffchange-inline">library </del>of <del class="diffchange diffchange-inline">regulatory </del>gene <del class="diffchange diffchange-inline">expression elements </del>that <del class="diffchange diffchange-inline">allow </del>for compression of multiple coding sequences into a smaller amount of genetic space. <del class="diffchange diffchange-inline">Using a pseudoknot </del>RNA structural motif, <del class="diffchange diffchange-inline">commonly </del>used <del class="diffchange diffchange-inline">by viruses to minimize their genome size, we will show the utility </del>of <del class="diffchange diffchange-inline">dual-coding gene sequences to give useful protein products whose expression can be </del>regulated <del class="diffchange diffchange-inline">by </del>the pseudoknot’s ability to induce ribosomal frameshifting. A software tool <del class="diffchange diffchange-inline">will </del>also <del class="diffchange diffchange-inline">be used </del>to <del class="diffchange diffchange-inline">zip </del>multiple coding sequences into <del class="diffchange diffchange-inline">different </del>reading frames. Ultimately, this library of standardized parts will be available for use in a variety of engineered systems requiring minimal coding space and multiple protein expression.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black">The current growth in synthetic biology research promises more complex and useful engineered systems. However, increased complexity often requires more genetic material that can be difficult to introduce into organisms. <ins class="diffchange diffchange-inline">The 2013 Lethbridge iGEM FRAMEchanger project has produced </ins>a new <ins class="diffchange diffchange-inline">class </ins>of gene <ins class="diffchange diffchange-inline">regulatory parts </ins>that <ins class="diffchange diffchange-inline">can be used </ins>for compression of multiple coding sequences into a smaller amount of genetic space. <ins class="diffchange diffchange-inline">This is achieved by programmed ribosomal frameshifting induced by the </ins>RNA structural motif <ins class="diffchange diffchange-inline">called a pseudoknot. Addtionally</ins>, <ins class="diffchange diffchange-inline">this part can by </ins>used <ins class="diffchange diffchange-inline">for variable tagging </ins>of <ins class="diffchange diffchange-inline">proteins, </ins>regulated <ins class="diffchange diffchange-inline">expression of proteins in an operon, and other applications in </ins>the <ins class="diffchange diffchange-inline">field of synthetic biology. The </ins>pseudoknot’s ability to induce ribosomal frameshifting <ins class="diffchange diffchange-inline">can be modulated by changing the stability of the pseudoknot structure. In this way, a library of frameshifting parts with variable frameshifting frequencies can be made available for a variety of applications</ins>. A software tool <ins class="diffchange diffchange-inline">has </ins>also <ins class="diffchange diffchange-inline">been developed </ins>to <ins class="diffchange diffchange-inline">facilitate zipping </ins>multiple coding sequences into <ins class="diffchange diffchange-inline">overlapped </ins>reading frames. Ultimately, this library of standardized parts will be available for use in a variety of engineered systems requiring minimal coding space and multiple protein expression.</p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHAT?</u></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHAT?</u></b></div></td></tr>
</table>Jennafriedthttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=237457&oldid=prevDustin at 02:49, 28 September 20132013-09-28T02:49:26Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="<del class="diffchange diffchange-inline">750px</del>" height="<del class="diffchange diffchange-inline">500px</del>"/></a></center></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="<ins class="diffchange diffchange-inline">600px</ins>" height="<ins class="diffchange diffchange-inline">400px</ins>"/></a></center></div></td></tr>
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</table>Dustinhttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=235012&oldid=prevDustin at 02:04, 28 September 20132013-09-28T02:04:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TeamLethbridgeHead}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{TeamLethbridgeHead}}</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="750px" height="500px"/></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://2013.igem.org/Team:Lethbridge/project"><image src="https://static.igem.org/mediawiki/2013/0/05/ULeth2013iGEM_Mainpage_PKFig.png" width="750px" height="500px"/></a></center></div></td></tr>
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</table>Dustinhttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=234041&oldid=prevHarlando at 01:50, 28 September 20132013-09-28T01:50:12Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHY?</u></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>WHY?</u></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>HOW?</b></u></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b style="color:black"><u>HOW?</b></u></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>We have brought pseudoknots to the iGEM community by: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul style="color:black"><li>We have brought pseudoknots to the iGEM community by: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li>Characterizing their function in a biobrick system</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ul><li>Characterizing their function in a biobrick system</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>Designing software that enables others to dual code proteins</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>Designing software that enables others to dual code proteins</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world</li></li></ul></ul></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>Ensuring that the release of these tools to the wider public does not pose a significant risk to the rest of the world</li></li></ul></ul<ins class="diffchange diffchange-inline">><br</ins>></div></td></tr>
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</table>Harlandohttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=233885&oldid=prevHarlando at 01:47, 28 September 20132013-09-28T01:47:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black">The current growth in synthetic biology research promises more complex and useful engineered systems. However, increased complexity often requires more genetic material that can be difficult to introduce into organisms. We propose the development of a new library of regulatory gene expression elements that allow for compression of multiple coding sequences into a smaller amount of genetic space. Using a pseudoknot RNA structural motif, commonly used by viruses to minimize their genome size, we will show the utility of dual-coding gene sequences to give useful protein products whose expression can be regulated by the pseudoknot’s ability to induce ribosomal frameshifting. A software tool will also be used to zip multiple coding sequences into different reading frames. Ultimately, this library of standardized parts will be available for use in a variety of engineered systems requiring minimal coding space and multiple protein expression.</p><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black">The current growth in synthetic biology research promises more complex and useful engineered systems. However, increased complexity often requires more genetic material that can be difficult to introduce into organisms. We propose the development of a new library of regulatory gene expression elements that allow for compression of multiple coding sequences into a smaller amount of genetic space. Using a pseudoknot RNA structural motif, commonly used by viruses to minimize their genome size, we will show the utility of dual-coding gene sequences to give useful protein products whose expression can be regulated by the pseudoknot’s ability to induce ribosomal frameshifting. A software tool will also be used to zip multiple coding sequences into different reading frames. Ultimately, this library of standardized parts will be available for use in a variety of engineered systems requiring minimal coding space and multiple protein expression.</p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b style="color:black"><u>WHAT?</u></b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><ul style="color:black"><li>Our project is directed towards standardizing pseudoknots to make a new class of parts available to the synthetic biology community</li></ul> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b style="color:black"><u>WHY?</u></b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><ul style="color:black"><li>As the field of synthetic biology grows, so should its toolset. By introducing a standardized method of implementing programmed ribosomal frameshifts in synthetic gene networks, we could not only enable others to reduce plasmid size and regulate operon expression, but also enable them to come up with new, exciting applications</li></ul></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b style="color:black"><u>HOW?</b></u></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><ul style="color:black"><li>We have brought pseudoknots to the iGEM community by: </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><ul><li>Characterizing their function in a biobrick system</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>Designing software that enables others to dual code proteins</li></ins></div></td></tr>
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</table>Harlandohttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=231441&oldid=prevHarlando at 00:58, 28 September 20132013-09-28T00:58:38Z<p></p>
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</table>Harlandohttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=231392&oldid=prevDustin at 00:57, 28 September 20132013-09-28T00:57:45Z<p></p>
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</table>Dustinhttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=231158&oldid=prevDustin at 00:52, 28 September 20132013-09-28T00:52:28Z<p></p>
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</table>Dustinhttp://2013.igem.org/wiki/index.php?title=Team:Lethbridge&diff=231120&oldid=prevHarlando at 00:51, 28 September 20132013-09-28T00:51:54Z<p></p>
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