http://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&feed=atom&action=historyTeam:MIT/Protein - Revision history2024-03-29T08:06:03ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=358562&oldid=prevClescott at 02:22, 29 October 20132013-10-29T02:22:03Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Shen, B et al. Protein targeting to exosomes/microvesicles by plasma membrane anchors. J Biol Chem. (2011) </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Shen, B et al. Protein targeting to exosomes/microvesicles by plasma membrane anchors. J Biol Chem. (2011) </div></td></tr>
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</table>Clescotthttp://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=358493&oldid=prevClescott at 02:19, 29 October 20132013-10-29T02:19:43Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Overview of Protein-based Cell-Cell Communication</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Overview of Protein-based Cell-Cell Communication</h1></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>While miRNA are useful in their own respect, it’s not currently known how miR-451 is naturally targeted into exosomes, making it difficult to engineer a system of sending any generic RNA. Luckily, research has shown that proteins can be targeted into exosomes by using an oligomerizing protein domain called Acyl-TyA. By fusing our protein signal to an Acyl-TyA domain, it’s possible to target our signal protein into exosomes and send it to another cell to evoke a response. <br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>While miRNA are useful in their own respect, it’s not currently known how miR-451 is naturally targeted into exosomes, making it difficult to engineer a system of sending any generic RNA. Luckily, research has shown that proteins can be targeted into exosomes by using an oligomerizing protein domain called Acyl-TyA <ins class="diffchange diffchange-inline">(Shen, 2011)</ins>. By fusing our protein signal to an Acyl-TyA domain, it’s possible to target our signal protein into exosomes and send it to another cell to evoke a response. <br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Shen, B et al. Protein targeting to exosomes/microvesicles by plasma membrane anchors. J Biol Chem. (2011) </ins></div></td></tr>
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</table>Clescotthttp://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=356412&oldid=prevNhall at 01:00, 29 October 20132013-10-29T01:00:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Overview of Protein-based Cell-Cell Communication</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Overview of Protein-based Cell-Cell Communication</h1></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>While miRNA are useful in their own respect, it’s not currently known how miR-451 is naturally targeted into exosomes, making it difficult to engineer a system of sending any generic RNA. Luckily, research has shown that proteins can be targeted into exosomes by using an oligomerizing protein domain called Acyl-TyA. By fusing our protein signal to an Acyl-TyA domain, it’s possible to target our signal protein into exosomes and send it to another cell to evoke a response. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>While miRNA are useful in their own respect, it’s not currently known how miR-451 is naturally targeted into exosomes, making it difficult to engineer a system of sending any generic RNA. Luckily, research has shown that proteins can be targeted into exosomes by using an oligomerizing protein domain called Acyl-TyA. By fusing our protein signal to an Acyl-TyA domain, it’s possible to target our signal protein into exosomes and send it to another cell to evoke a response. <ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </div></td></tr>
</table>Nhallhttp://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=356395&oldid=prevNhall at 01:00, 29 October 20132013-10-29T01:00:21Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h1>Overview of Protein-based Cell-Cell Communication</h1></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">While miRNA are useful in their own respect, it’s not currently known how miR-451 is naturally targeted into exosomes, making it difficult to engineer a system of sending any generic RNA. Luckily, research has shown that proteins can be targeted into exosomes by using an oligomerizing protein domain called Acyl-TyA. By fusing our protein signal to an Acyl-TyA domain, it’s possible to target our signal protein into exosomes and send it to another cell to evoke a response. </ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Our goal is to use Acyl-TyA to target generic protein signals into exosomes which allow for cell-cell communication by facilitating the transfer of a signal protein from a sender cell to a receiver cell. The general procedure begins with creating an Acyl-TyA fusion protein and testing the signal’s function within single cells to determine whether the fusion was successful. Then, once the signal’s function is verified, we use Jurkat T cells to produce exosomes containing our signal and apply these exosomes to receiver cells—the signal would then enter the receiver cells via the exosomes and activate a specific receiver circuit. Finally, the signal producing Jurkat T cells are to be cocultured together with receiver cells to determine whether cell-cell communication has been achieved. </ins></div></td></tr>
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</table>Nhallhttp://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=350510&oldid=prevKlathem at 19:35, 28 October 20132013-10-28T19:35:51Z<p></p>
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</table>Klathemhttp://2013.igem.org/wiki/index.php?title=Team:MIT/Protein&diff=350503&oldid=prevKlathem: Created page with "{{MIT-results2}} <html> <head> <script> document.title = "MIT iGEM - Protein Signals Overview"; $(document).ready(function() { $("#accordion").accordion("option", "animated",..."2013-10-28T19:35:37Z<p>Created page with "{{MIT-results2}} <html> <head> <script> document.title = "MIT iGEM - Protein Signals Overview"; $(document).ready(function() { $("#accordion").accordion("option", "animated",..."</p>
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