http://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&feed=atom&action=historyTeam:MSOE Milwaukee/Week3 - Revision history2024-03-29T07:47:34ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=83150&oldid=prevVanhandelp at 13:40, 22 August 20132013-08-22T13:40:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><FONT color = 'green' size="+20">Week 3</FONT><BR><BR></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><FONT color = 'green' size="+20">Week 3</FONT><BR><BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">style="text-align:justify"</ins>>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>In small groups we researched the different pathways and enzymes involved in the output of Eucalyptol and found the pathways incorporated in its production. The glucose from the spent grains will travel into the citric acid cycle, then through the mevalonate pathway, and then a final enzyme will synthesize the Eucalyptol from Geranyl-PP. We found the different enzymes incorporated in the different pathways that do not already exist in </i>E.coli</i> and found their sequences in online databases.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">style="text-align:justify"</ins>>In small groups we researched the different pathways and enzymes involved in the output of Eucalyptol and found the pathways incorporated in its production. The glucose from the spent grains will travel into the citric acid cycle, then through the mevalonate pathway, and then a final enzyme will synthesize the Eucalyptol from Geranyl-PP. We found the different enzymes incorporated in the different pathways that do not already exist in </i>E.coli</i> and found their sequences in online databases.</p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>To continue our research, we looked at different promoters, terminators, and plasmids in the parts registry that are available for our use. We also looked at past iGEM projects to investigate which ones they used and their reasoning behind that to help us make our decision. We decided on using the T7 promoter to control the expression of our genes and the standard double terminator.</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">style="text-align:justify"</ins>>To continue our research, we looked at different promoters, terminators, and plasmids in the parts registry that are available for our use. We also looked at past iGEM projects to investigate which ones they used and their reasoning behind that to help us make our decision. We decided on using the T7 promoter to control the expression of our genes and the standard double terminator.</p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>In groups, we researched the possible locations of where to incorporate terminators and promoters in the biobrick parts, and we decided to use one promoter and one terminator for each biobrick part or gene to promote stable gene expression. We also decided to use three genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. Our plan is to group our output genes as follows:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">style="text-align:justify"</ins>>In groups, we researched the possible locations of where to incorporate terminators and promoters in the biobrick parts, and we decided to use one promoter and one terminator for each biobrick part or gene to promote stable gene expression. We also decided to use three genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. Our plan is to group our output genes as follows:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>Individually, we further researched plasmids, promoters, and the strains of </i>E.coli</i>. We determined that using the DE3 or the BL21 strain of </i>E.coli</i> would be most beneficial to us because it is an expression strain and is compatible with the the T7 promoter we would like to use. We also determined that we would like to continue our research on plasmids, but that the pSTV28 plasmid vector was of itnerest because it is a low copy plasmid and more suited to our use than the standard plasmids available in the iGEM kit. </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p <ins class="diffchange diffchange-inline">style="text-align:justify"</ins>>Individually, we further researched plasmids, promoters, and the strains of </i>E.coli</i>. We determined that using the DE3 or the BL21 strain of </i>E.coli</i> would be most beneficial to us because it is an expression strain and is compatible with the the T7 promoter we would like to use. We also determined that we would like to continue our research on plasmids, but that the pSTV28 plasmid vector was of itnerest because it is a low copy plasmid and more suited to our use than the standard plasmids available in the iGEM kit. </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:MSOE:Notebook}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Team:MSOE:Notebook}}</div></td></tr>
</table>Vanhandelphttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=48067&oldid=prevVanhandelp at 00:24, 31 July 20132013-07-31T00:24:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><FONT color = 'green' size="+20">Week <del class="diffchange diffchange-inline">1</del></FONT><BR><BR></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><FONT color = 'green' size="+20">Week <ins class="diffchange diffchange-inline">3</ins></FONT><BR><BR></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td></tr>
</table>Vanhandelphttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=48066&oldid=prevVanhandelp at 00:23, 31 July 20132013-07-31T00:23:49Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><FONT color = 'green' size="+20">Week 1</FONT><BR><BR></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p>As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</p><br></div></td></tr>
</table>Vanhandelphttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=19694&oldid=prevMajewskir at 18:36, 24 June 20132013-06-24T18:36:15Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p>In groups, we researched the possible locations of where to incorporate terminators and promoters in the biobrick parts, and we decided to use one promoter and one terminator for each biobrick part or gene to promote stable gene expression. We also decided to use <del class="diffchange diffchange-inline">two </del>genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. Our plan is to group our output genes as follows:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p>In groups, we researched the possible locations of where to incorporate terminators and promoters in the biobrick parts, and we decided to use one promoter and one terminator for each biobrick part or gene to promote stable gene expression. We also decided to use <ins class="diffchange diffchange-inline">three </ins>genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. Our plan is to group our output genes as follows:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Plasmid 1: HMG-CoA synthase <del class="diffchange diffchange-inline">and </del>HMG-CoA reductase <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Plasmid 1: HMG-CoA synthase<ins class="diffchange diffchange-inline">, </ins>HMG-CoA reductase<ins class="diffchange diffchange-inline">, and Mevalonate kinase</ins><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>*Plasmid 2: <del class="diffchange diffchange-inline">Mevalonate kinase and </del>Phosphomevalonate kinase <del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>*Plasmid 2: Phosphomevalonate kinase<ins class="diffchange diffchange-inline">, </ins>Mevalonate-5-pyrophosphate decarboxylase<ins class="diffchange diffchange-inline">, </ins>and 1,8-cineole synthase<br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">*Plasmid 3: </del>Mevalonate-5-pyrophosphate decarboxylase and 1,8-cineole synthase<del class="diffchange diffchange-inline"></p></del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">Individually, we further researched plasmids, promoters, and the strains of </i>E.coli</i>. We determined that using the DE3 or the BL21 strain of </i>E.coli</i> would be most beneficial to us because it is an expression strain and is compatible with the the T7 promoter we would like to use. We also determined that we would like to continue our research on plasmids, but that the pSTV28 plasmid vector was of itnerest because it is a low copy plasmid and more suited to our use than the standard plasmids available in the iGEM kit. </ins></p></div></td></tr>
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</table>Majewskirhttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=17835&oldid=prevVanhandelp at 22:08, 20 June 20132013-06-20T22:08:57Z<p></p>
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</table>Vanhandelphttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=17554&oldid=prevMajewskir at 15:36, 20 June 20132013-06-20T15:36:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">As a team, we finally decided on inputs and outputs for our project. After a long discussion, we chose Eucalyptol as our output because we wanted a more rare or hard to obtain substance. For our input, we chose to use the spent grains from beer production here in Milwaukee. This provided us with direction to further our research on the pathways and enzymes involved.</ins></p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">In small groups we researched the different pathways and enzymes involved in the output of Eucalyptol and found the pathways incorporated in its production. The glucose from the spent grains will travel into the citric acid cycle, then through the mevalonate pathway, and then a final enzyme will synthesize the Eucalyptol from Geranyl-PP. We found the different enzymes incorporated in the different pathways that do not already exist in </i>E.coli</i> and found their sequences in online databases.</ins></p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">To continue our research, we looked at different promoters, terminators, and plasmids in the parts registry that are available for our use. We also looked at past iGEM projects to investigate which ones they used and their reasoning behind that to help us make our decision. We decided on using the T7 promoter to control the expression of our genes and the standard double terminator.</ins></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p> </p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">In groups, we researched the possible locations of where to incorporate terminators and promoters in the biobrick parts, and we decided to use one promoter and one terminator for each biobrick part or gene to promote stable gene expression. We also decided to use two genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. Our plan is to group our output genes as follows:</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Plasmid 1: HMG-CoA synthase and HMG-CoA reductase <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Plasmid 2: Mevalonate kinase and Phosphomevalonate kinase <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">*Plasmid 3: Mevalonate-5-pyrophosphate decarboxylase and 1,8-cineole synthase</ins></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td></tr>
</table>Majewskirhttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=17075&oldid=prevReichartzm at 00:47, 20 June 20132013-06-20T00:47:26Z<p></p>
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</table>Reichartzmhttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=15768&oldid=prevVanhandelp at 22:40, 17 June 20132013-06-17T22:40:40Z<p></p>
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</table>Vanhandelphttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=15723&oldid=prevReichartzm at 22:14, 17 June 20132013-06-17T22:14:56Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">As a team, we discussed our research on the different ideas developed in Week 1 and went through a voting process to decide on our final topic. Our votes tied numerous times as we attempted to decide, but eventually agreed that our project idea was going to be the biosynthesis of fragrances in </i>E.coli</i>.</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">In small groups, we researched three possible inputs and three possible outputs for the biosynthesis of fragrances in </i>E.coli</i>. We discussed many input options, including using blue light and using a waste product. For outputs, we discussed developing fragrances or flavorings, including patchoulol, geraniol, linalool, and cinnamaldehyde. Pathways and enzymes involving these molecules were researched at length to try to find a next step for our project.</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p></p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">As the inputs and outputs of our project were being researched, a potential project idea developed. We decided to further research the biosynthesis of cinnamaldehyde through the pentose phosphate pathway and the shikimate pathway. These pathways were chosen because the inputs can come directly from the spent grains used in beer production here in Milwaukee. The glucose from these grains can be used in these pathways to produce phenylalanine, which can then be used to produce the desired product cinnamaldehyde.</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p></p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Individually, the different possible inputs were researched. More was discovered about using spent grains from a brewery here in Milwaukee. </del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p> </p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">After a discussion with our advisor, we realized that we needed to come up with an output of a more rare, or more in demand fragrance or oil. Cinnamaldehyde was just too common of a fragrance to focus our project on, so we found a list of alternative oils to research, including Eucalyptus oil and Saffron. Hopefully we can narrow down the list on Monday. </del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p></p></div></td></tr>
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</table>Reichartzmhttp://2013.igem.org/wiki/index.php?title=Team:MSOE_Milwaukee/Week3&diff=15721&oldid=prevVanhandelp at 22:14, 17 June 20132013-06-17T22:14:00Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> <li><a href="/Team:MSOE_Milwaukee/Week1">Week 1</a></li> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <body></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <body></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Monday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Text for Monday</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">As a team, we discussed our research on the different ideas developed in Week 1 and went through a voting process to decide on our final topic. Our votes tied numerous times as we attempted to decide, but eventually agreed that our project idea was going to be the biosynthesis of fragrances in </i>E.coli</i>.</ins></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Tuesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Text </del>for <del class="diffchange diffchange-inline">Tuesday</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">In small groups, we researched three possible inputs and three possible outputs </ins>for <ins class="diffchange diffchange-inline">the biosynthesis of fragrances in </i>E.coli</i>. We discussed many input options, including using blue light and using a waste product. For outputs, we discussed developing fragrances or flavorings, including patchoulol, geraniol, linalool, and cinnamaldehyde. Pathways and enzymes involving these molecules were researched at length to try to find a next step for our project.</ins></p><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Wednesday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Text for Wednesday</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">As the inputs and outputs of our project were being researched, a potential project idea developed. We decided to further research the biosynthesis of cinnamaldehyde through the pentose phosphate pathway and the shikimate pathway. These pathways were chosen because the inputs can come directly from the spent grains used in beer production here in Milwaukee. The glucose from these grains can be used in these pathways to produce phenylalanine, which can then be used to produce the desired product cinnamaldehyde.</ins></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Thursday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Text for Thursday</del></p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">Individually, the different possible inputs were researched. More was discovered about using spent grains from a brewery here in Milwaukee. </ins></p><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <H1 align = left>Friday</H1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p><del class="diffchange diffchange-inline">Text for Friday</del></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p><ins class="diffchange diffchange-inline">After a discussion with our advisor, we realized that we needed to come up with an output of a more rare, or more in demand fragrance or oil. Cinnamaldehyde was just too common of a fragrance to focus our project on, so we found a list of alternative oils to research, including Eucalyptus oil and Saffron. Hopefully we can narrow down the list on Monday. </ins></p></div></td></tr>
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