http://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&feed=atom&action=historyTeam:Manaus Amazonas-Brazil/Project - Revision history2024-03-29T13:06:40ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&diff=62317&oldid=prevCgmanaus: /* Overall project */2013-08-08T21:22:50Z<p><span class="autocomment">Overall project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Have you ever tasted those delicious French-fries from your favorite fast food restaurant or even at home watching that nice movie? Or have you lately ate some grilled hamburger, fried fish or nuggets?…Humm, ok you might think I’m just hungry and this is nothing to do with an iGEM project, right? Totally wrong! Yes… it is a Synthetic Biology project and is all about our daily life, better saying it’s about our daily waste, and not to mention, one of the most environment contaminating wastes: used frying oil. It is produced in deep-fried food preparations mainly in bars, restaurants, industrial kitchens, hotels and even in our own homes, and its production has tremendously increased over the last years. We are disposing approximately 146 million tons of used frying oil per year. However, do you know the final destination of it? Well, many (yes many) people (I mean 90% of the population) still doesn’t know, and even worse: we do not know what to do with the frying residual oil and ending up discarding it without any pre-treatment, therefore, polluting rivers, lakes, oceans and soils, killing all sorts of living organisms and messing up ecosystems. Some actions have been done since much time ago trying to remove or treat this kind of waste, such as making soap or more recently taking this frying oil residue and turn into biofuel. Great options but still very timid when we think the amount of used frying oil production every year (and scaling up), and the need of to be rid of this waste. The equation for the environmental contamination by residual oils is like that: up to one million liters of water for each liter of oil! Looking at this huge humanity problem we came with a SynBio project for this year’s iGEM competition. We saw a great potential in using Synthetic Biology for tackling not just the used frying oil waste but on top of that turn this waste electric energy. Yeap, you read that right! To be more precisely, an electric power plant will be set into bacterias. The high demand of electric energy, the shortage of fuels and search for renewable energy sources, and the rise of Synthetic Biology sets up a great opportunity for doing so. Aiming to provide an alternative to the reuse of this disposed oil we started “The Electrobacter” project, a bacteria capable of degrade used frying oil and turn it into electric power. Soon, the destiny of waste fats for energy generation will have a new bio-energy alternative through a Bioplant. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Have you ever tasted those delicious French-fries from your favorite fast food restaurant or even at home watching that nice movie? Or have you lately ate some grilled<ins class="diffchange diffchange-inline">/fried </ins>hamburger <ins class="diffchange diffchange-inline">meat</ins>, fried fish or nuggets?…Humm, ok you might think I’m just hungry and this is nothing to do with an iGEM project, right? Totally wrong! Yes… it is a Synthetic Biology project and is all about our daily life, better saying it’s about our daily waste, and not to mention, one of the most environment contaminating wastes: used frying oil. It is produced in deep-fried food preparations mainly in bars, restaurants, industrial kitchens, hotels and even in our own homes, and its production has tremendously increased over the last years. We are disposing approximately 146 million tons of used frying oil per year. However, do you know the final destination of it? Well, many (yes many) people (I mean 90% of the population) still doesn’t know, and even worse: we do not know what to do with the frying residual oil and ending up discarding it without any pre-treatment, therefore, polluting rivers, lakes, oceans and soils, killing all sorts of living organisms and messing up ecosystems. Some actions have been done since much time ago trying to remove or treat this kind of waste, such as making soap or more recently taking this frying oil residue and turn into biofuel. Great options but still very timid when we think the amount of used frying oil production every year (and scaling up), and the need of to be rid of this waste. The equation for the environmental contamination by residual oils is like that: up to one million liters of water for each liter of oil! Looking at this huge humanity problem we came with a SynBio project for this year’s iGEM competition. We saw a great potential in using Synthetic Biology for tackling not just the used frying oil waste but on top of that turn this waste electric energy. Yeap, you read that right! To be more precisely, an electric power plant will be set into bacterias. The high demand of electric energy, the shortage of fuels and search for renewable energy sources, and the rise of Synthetic Biology sets up a great opportunity for doing so. Aiming to provide an alternative to the reuse of this disposed oil we started “The Electrobacter” project, a bacteria capable of degrade used frying oil and turn it into electric power. Soon, the destiny of waste fats for energy generation will have a new bio-energy alternative through a Bioplant. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The Gram-negative and protobacteria Shewanella, is a spread genus included in Amazon River. It has the capacity of degrade fat acids with different chain sizes as carbon sources, through β-oxidation, due to enzymatic reactions. The expression of these enzymes is regulated by the product of the FadR gene. We are going to use a new chassi in iGEM and build new biobricks based on both: the Registry and the Amazonian biodiversity. We are modifying the metabolic expression of some key components in order to increase fat degradation using of course used-frying-oil. Shewanella bacterias also releases electrons to the media and from there we are able to “capture” it for electric energy production. For this, we´ll do:1) inhibition of the codifying gene of β-oxidation repressor protein (FadR), making it “hungrier” for fat acids; 2) the increasing FadL expression, responsible for the entrance of fat acids in the cell; and 3) high expression of FadD, responsible for the insertion of CoA in the fat acid. For the conversion of chemical energy into electric energy, the Shewanella naturally transfers electrons produced during the β-oxidation. In order to “capture” it we are building a MFC (Microbial Fuel cell), which is a device capable of generating electricity through electrons transfer to an internal circuit. In the near future homes and restaurants will be charged by wasted oil used to make French fries...<del class="diffchange diffchange-inline">who knows</del>?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The Gram-negative and protobacteria <ins class="diffchange diffchange-inline">''</ins>Shewanella<ins class="diffchange diffchange-inline">''</ins>, is a spread genus included in Amazon River. It has the capacity of degrade fat acids with different chain sizes as carbon sources, through β-oxidation, due to enzymatic reactions. The expression of these enzymes is regulated by the product of the FadR gene. We are going to use a new chassi in iGEM and build new biobricks based on both: the Registry and the Amazonian biodiversity. We are modifying the metabolic expression of some key components in order to increase fat degradation using of course used-frying-oil. Shewanella bacterias also releases electrons to the media and from there we are able to “capture” it for electric energy production. For this, we´ll do:1) inhibition of the codifying gene of β-oxidation repressor protein (FadR), making it “hungrier” for fat acids; 2) the increasing FadL expression, responsible for the entrance of fat acids in the cell; and 3) high expression of FadD, responsible for the insertion of CoA in the fat acid. For the conversion of chemical energy into electric energy, the <ins class="diffchange diffchange-inline">''</ins>Shewanella<ins class="diffchange diffchange-inline">'' </ins>naturally transfers electrons produced during the β-oxidation. In order to “capture” it we are building a MFC (Microbial Fuel cell), which is a device capable of generating electricity through electrons transfer to an internal circuit. In the near future homes and restaurants will be charged by wasted oil used to make French fries...<ins class="diffchange diffchange-inline">by a Bacteria...awesome, wouldn't be</ins>?</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td></tr>
</table>Cgmanaushttp://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&diff=62236&oldid=prevCgmanaus: /* Overall project */2013-08-08T20:39:46Z<p><span class="autocomment">Overall project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Have you ever tasted those delicious French fries from your favorite fast food restaurant or even at home watching that nice movie? Or have you lately ate some grilled hamburger, fried fish or nuggets?…Humm, ok you might think I’m just hungry and this is nothing to do with an iGEM project, right? Totally wrong! Yes… it is a Synthetic Biology project and is all about our daily life, better saying it’s about our daily waste, and not to mention, one of the most environment contaminating wastes: used frying oil. It is produced in deep-fried food preparations mainly in bars, restaurants, industrial kitchens, hotels and even in our own homes, and its production has tremendously increased over the last years. We are disposing approximately 146 million tons of used frying oil per year. However, do you know the final destination of it? Well, many (yes many) people (I mean 90% of the population) still doesn’t know, and even worse: we do not know what to do with the frying residual oil and ending up discarding it without any pre-treatment, therefore, polluting rivers, lakes, oceans and soils, killing all sorts of living organisms and messing up ecosystems. Some actions have been done since much time ago trying to remove or treat this kind of waste, such as making soap or more recently taking this frying oil residue and turn into biofuel. Great options but still very timid when we think the amount of used frying oil production every year (and scaling up), and the need of to be rid of this waste. The equation for the environmental contamination by residual oils is like that: up to one million liters of water for each liter of oil! Looking at this huge humanity problem we came with a SynBio project for this year’s iGEM competition. We saw a great potential in using Synthetic Biology for tackling not just the used frying oil waste but on top of that turn this waste electric energy. Yeap, you read that right! To be more precisely, an electric power plant will be set into bacterias. The high demand of electric energy, the shortage of fuels and search for renewable energy sources, and the rise of Synthetic Biology sets up a great opportunity for doing so. Aiming to provide an alternative to the reuse of this disposed oil we started “The Electrobacter” project, a bacteria capable of degrade used frying oil and turn it into electric power. Soon, the destiny of waste fats for energy generation will have a new bio-energy alternative through a Bioplant. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Have you ever tasted those delicious French<ins class="diffchange diffchange-inline">-</ins>fries from your favorite fast food restaurant or even at home watching that nice movie? Or have you lately ate some grilled hamburger, fried fish or nuggets?…Humm, ok you might think I’m just hungry and this is nothing to do with an iGEM project, right? Totally wrong! Yes… it is a Synthetic Biology project and is all about our daily life, better saying it’s about our daily waste, and not to mention, one of the most environment contaminating wastes: used frying oil. It is produced in deep-fried food preparations mainly in bars, restaurants, industrial kitchens, hotels and even in our own homes, and its production has tremendously increased over the last years. We are disposing approximately 146 million tons of used frying oil per year. However, do you know the final destination of it? Well, many (yes many) people (I mean 90% of the population) still doesn’t know, and even worse: we do not know what to do with the frying residual oil and ending up discarding it without any pre-treatment, therefore, polluting rivers, lakes, oceans and soils, killing all sorts of living organisms and messing up ecosystems. Some actions have been done since much time ago trying to remove or treat this kind of waste, such as making soap or more recently taking this frying oil residue and turn into biofuel. Great options but still very timid when we think the amount of used frying oil production every year (and scaling up), and the need of to be rid of this waste. The equation for the environmental contamination by residual oils is like that: up to one million liters of water for each liter of oil! Looking at this huge humanity problem we came with a SynBio project for this year’s iGEM competition. We saw a great potential in using Synthetic Biology for tackling not just the used frying oil waste but on top of that turn this waste electric energy. Yeap, you read that right! To be more precisely, an electric power plant will be set into bacterias. The high demand of electric energy, the shortage of fuels and search for renewable energy sources, and the rise of Synthetic Biology sets up a great opportunity for doing so. Aiming to provide an alternative to the reuse of this disposed oil we started “The Electrobacter” project, a bacteria capable of degrade used frying oil and turn it into electric power. Soon, the destiny of waste fats for energy generation will have a new bio-energy alternative through a Bioplant. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Gram-negative and protobacteria Shewanella, is a spread genus included in Amazon River. It has the capacity of degrade fat acids with different chain sizes as carbon sources, through β-oxidation, due to enzymatic reactions. The expression of these enzymes is regulated by the product of the FadR gene. We are going to use a new chassi in iGEM and build new biobricks based on both: the Registry and the Amazonian biodiversity. We are modifying the metabolic expression of some key components in order to increase fat degradation using of course used-frying-oil. Shewanella bacterias also releases electrons to the media and from there we are able to “capture” it for electric energy production. For this, we´ll do:1) inhibition of the codifying gene of β-oxidation repressor protein (FadR), making it “hungrier” for fat acids; 2) the increasing FadL expression, responsible for the entrance of fat acids in the cell; and 3) high expression of FadD, responsible for the insertion of CoA in the fat acid. For the conversion of chemical energy into electric energy, the Shewanella naturally transfers electrons produced during the β-oxidation. In order to “capture” it we are building a MFC (Microbial Fuel cell), which is a device capable of generating electricity through electrons transfer to an internal circuit. In the near future homes and restaurants will be charged by wasted oil used to make French fries...who knows?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The Gram-negative and protobacteria Shewanella, is a spread genus included in Amazon River. It has the capacity of degrade fat acids with different chain sizes as carbon sources, through β-oxidation, due to enzymatic reactions. The expression of these enzymes is regulated by the product of the FadR gene. We are going to use a new chassi in iGEM and build new biobricks based on both: the Registry and the Amazonian biodiversity. We are modifying the metabolic expression of some key components in order to increase fat degradation using of course used-frying-oil. Shewanella bacterias also releases electrons to the media and from there we are able to “capture” it for electric energy production. For this, we´ll do:1) inhibition of the codifying gene of β-oxidation repressor protein (FadR), making it “hungrier” for fat acids; 2) the increasing FadL expression, responsible for the entrance of fat acids in the cell; and 3) high expression of FadD, responsible for the insertion of CoA in the fat acid. For the conversion of chemical energy into electric energy, the Shewanella naturally transfers electrons produced during the β-oxidation. In order to “capture” it we are building a MFC (Microbial Fuel cell), which is a device capable of generating electricity through electrons transfer to an internal circuit. In the near future homes and restaurants will be charged by wasted oil used to make French fries...who knows?</div></td></tr>
</table>Cgmanaushttp://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&diff=62226&oldid=prevCgmanaus: /* Overall project */2013-08-08T20:37:05Z<p><span class="autocomment">Overall project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Overall project''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Tell us more about </del>your project. <del class="diffchange diffchange-inline"> Give us background</del>. <del class="diffchange diffchange-inline"> Use </del>this is the <del class="diffchange diffchange-inline">abstract </del>of <del class="diffchange diffchange-inline">your </del>project. <del class="diffchange diffchange-inline">Be descriptive but concise (</del>1-2 <del class="diffchange diffchange-inline">paragraphs</del>)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Have you ever tasted those delicious French fries from </ins>your <ins class="diffchange diffchange-inline">favorite fast food restaurant or even at home watching that nice movie? Or have you lately ate some grilled hamburger, fried fish or nuggets?…Humm, ok you might think I’m just hungry and this is nothing to do with an iGEM </ins>project<ins class="diffchange diffchange-inline">, right? Totally wrong! Yes… it is a Synthetic Biology project and is all about our daily life, better saying it’s about our daily waste, and not to mention, one of the most environment contaminating wastes: used frying oil</ins>. <ins class="diffchange diffchange-inline">It is produced in deep-fried food preparations mainly in bars, restaurants, industrial kitchens, hotels and even in our own homes, and its production has tremendously increased over the last years</ins>. <ins class="diffchange diffchange-inline">We are disposing approximately 146 million tons of used frying oil per year. However, do you know the final destination of it? Well, many (yes many) people (I mean 90% of the population) still doesn’t know, and even worse: we do not know what to do with the frying residual oil and ending up discarding it without any pre-treatment, therefore, polluting rivers, lakes, oceans and soils, killing all sorts of living organisms and messing up ecosystems. Some actions have been done since much time ago trying to remove or treat </ins>this <ins class="diffchange diffchange-inline">kind of waste, such as making soap or more recently taking this frying oil residue and turn into biofuel. Great options but still very timid when we think the amount of used frying oil production every year (and scaling up), and the need of to be rid of this waste. The equation for the environmental contamination by residual oils </ins>is <ins class="diffchange diffchange-inline">like that: up to one million liters of water for each liter of oil! Looking at this huge humanity problem we came with a SynBio project for this year’s iGEM competition. We saw a great potential in using Synthetic Biology for tackling not just </ins>the <ins class="diffchange diffchange-inline">used frying oil waste but on top </ins>of <ins class="diffchange diffchange-inline">that turn this waste electric energy. Yeap, you read that right! To be more precisely, an electric power plant will be set into bacterias. The high demand of electric energy, the shortage of fuels and search for renewable energy sources, and the rise of Synthetic Biology sets up a great opportunity for doing so. Aiming to provide an alternative to the reuse of this disposed oil we started “The Electrobacter” </ins>project<ins class="diffchange diffchange-inline">, a bacteria capable of degrade used frying oil and turn it into electric power. Soon, the destiny of waste fats for energy generation will have a new bio-energy alternative through a Bioplant</ins>. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The Gram-negative and protobacteria Shewanella, is a spread genus included in Amazon River. It has the capacity of degrade fat acids with different chain sizes as carbon sources, through β-oxidation, due to enzymatic reactions. The expression of these enzymes is regulated by the product of the FadR gene. We are going to use a new chassi in iGEM and build new biobricks based on both: the Registry and the Amazonian biodiversity. We are modifying the metabolic expression of some key components in order to increase fat degradation using of course used-frying-oil. Shewanella bacterias also releases electrons to the media and from there we are able to “capture” it for electric energy production. For this, we´ll do:</ins>1<ins class="diffchange diffchange-inline">) inhibition of the codifying gene of β</ins>-<ins class="diffchange diffchange-inline">oxidation repressor protein (FadR), making it “hungrier” for fat acids; </ins>2) <ins class="diffchange diffchange-inline">the increasing FadL expression, responsible for the entrance of fat acids in the cell; and 3) high expression of FadD, responsible for the insertion of CoA in the fat acid. For the conversion of chemical energy into electric energy, the Shewanella naturally transfers electrons produced during the β-oxidation. In order to “capture” it we are building a MFC (Microbial Fuel cell), which is a device capable of generating electricity through electrons transfer to an internal circuit. In the near future homes and restaurants will be charged by wasted oil used to make French fries...who knows?</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Project Details==</div></td></tr>
</table>Cgmanaushttp://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&diff=19826&oldid=prevLunalacerda: /* Project Details */2013-06-24T20:50:34Z<p><span class="autocomment">Project Details</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Part 3 ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Part 3 ===</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Results ==</div></td></tr>
</table>Lunalacerdahttp://2013.igem.org/wiki/index.php?title=Team:Manaus_Amazonas-Brazil/Project&diff=1701&oldid=prevIGEM HQ: Prototype team page2013-04-16T19:22:33Z<p>Prototype team page</p>
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.<br />
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You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace. <br />
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil|Home]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Team|Team]]<br />
!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=Manaus_Amazonas-Brazil Official Team Profile]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Project|Project]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Parts|Parts Submitted to the Registry]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Modeling|Modeling]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Notebook|Notebook]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Safety|Safety]]<br />
!align="center"|[[Team:Manaus_Amazonas-Brazil/Attributions|Attributions]]<br />
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== '''Overall project''' ==<br />
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Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)<br />
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== Project Details==<br />
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=== Part 2 ===<br />
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=== The Experiments ===<br />
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=== Part 3 ===<br />
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== Results ==</div>IGEM HQ