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Team:Minnesota/Project
From 2013.igem.org
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<p>The 2013 University of Minnesota iGEM team aims to produce active human insulin by designing a versatile expression system in the industrially-relevant yeast, Pichia pastoris. The team will construct several novel expression systems and employ methanol-based induction systems using the standardized, modular design of the BioBricks systems. As well as this, the team will use cross-kingdom conjugation to advance the genetic toolkit available for transformation and expression in Pichia. Ultimately, we will utilize our system to produce active insulin as a proof-of-concept.</p> | <p>The 2013 University of Minnesota iGEM team aims to produce active human insulin by designing a versatile expression system in the industrially-relevant yeast, Pichia pastoris. The team will construct several novel expression systems and employ methanol-based induction systems using the standardized, modular design of the BioBricks systems. As well as this, the team will use cross-kingdom conjugation to advance the genetic toolkit available for transformation and expression in Pichia. Ultimately, we will utilize our system to produce active insulin as a proof-of-concept.</p> | ||
| - | <a href="https://2013.igem.org/Team:Minnesota/Project/ | + | <a href="https://2013.igem.org/Team:Minnesota/Project/Pichia_Expression_System"> |
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| - | <a href="https://2013.igem.org/Team:Minnesota/Project/ | + | <a href="https://2013.igem.org/Team:Minnesota/Project/Pichia_Expression_System"> |
<img style="position:absolute; top:125px;left:0px; display:none;" id="MainCaffeineButton2" src="http://imageshack.us/a/img202/7744/jyit.png"> | <img style="position:absolute; top:125px;left:0px; display:none;" id="MainCaffeineButton2" src="http://imageshack.us/a/img202/7744/jyit.png"> | ||
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| - | <a href="https://2013.igem.org/Team:Minnesota/Project/ | + | <a href="https://2013.igem.org/Team:Minnesota/Project/Insulin"> |
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| - | <a href="https://2013.igem.org/Team:Minnesota/Project/ | + | <a href="https://2013.igem.org/Team:Minnesota/Project/Insulin"> |
<img style="position:absolute; top:125px;left:325px; display:none;" id="MainSunscreenButton2" src="http://imageshack.us/a/img138/6616/4299.png"> | <img style="position:absolute; top:125px;left:325px; display:none;" id="MainSunscreenButton2" src="http://imageshack.us/a/img138/6616/4299.png"> | ||
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Revision as of 19:18, 1 September 2013
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The 2013 University of Minnesota iGEM team aims to produce active human insulin by designing a versatile expression system in the industrially-relevant yeast, Pichia pastoris. The team will construct several novel expression systems and employ methanol-based induction systems using the standardized, modular design of the BioBricks systems. As well as this, the team will use cross-kingdom conjugation to advance the genetic toolkit available for transformation and expression in Pichia. Ultimately, we will utilize our system to produce active insulin as a proof-of-concept.
