Team:NCTU Formosa/achievement

For light-regulated system

• Installed a “two component system(cph8)” that can respond to red light
• To construct a more directly sensor to red light, we designed a new promoter, P_red, which originated from P_ompC
• Improved the biobrick BBa_I15010, and constructed a functional counterpart, BBa_K1017301
• Proved that the P_red is able to work by experiment

For temperature-regulated system

• Designed a great RNA thermometer it can regulate the translation efficiency as temperature changing
• Proved that this system can work by comparing the GFP expression at different temperature

For small RNA-regulated system

• Designed a regulated system its inhibition work under RNA level
• Constructed a functional sRNA biobrick
• Constructed the target RBS of sRNA
• Proved that the target RBS can really work as a RBS and performed better than other iGEM common RBS
• Proved our small RNA-regulated system can really work

For Human Practice

• Held a conference in this summer time, many Chinese teams and Taiwanese teams participated this conference.
• Promoted Synthetic Biology communication in Asia by the conference
• Held a summer camp to senior high school students, teaching them many knowledge of Synthetic Biology
• Used a Chinese guiding book about iGEM to teach people who want to know Synthetic Biology
• Did a survey to understand the knowing Synthetic Biology level of the publics in Taiwan