Team:NJU NJUT China/data

From 2013.igem.org

(Difference between revisions)
(Created page with "<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta...")
 
(7 intermediate revisions not shown)
Line 25: Line 25:
     background-color:transparent;
     background-color:transparent;
     overflow:hide;
     overflow:hide;
 +
    position:absolute;
 +
z-index:-5;
}
}
#footer{
#footer{
Line 79: Line 81:
height:auto;
height:auto;
position:absolute;
position:absolute;
-
top:0px;
+
top:-15px;
-
left:0px;
+
left:-7px;
     z-index:0;
     z-index:0;
}
}
Line 159: Line 161:
overflow:scroll;
overflow:scroll;
overflow-x:hidden;
overflow-x:hidden;
 +
font-family: 'MS Sans Serif', Geneva, sans-serif;
 +
font-size: 15px;
}
}
Line 291: Line 295:
  <h2 class="title"><a href="#" >DATA</a></h2>
  <h2 class="title"><a href="#" >DATA</a></h2>
  <div class="entry">  
  <div class="entry">  
-
<center><img src="images/img1.png" width="80%" height="250px" border="0" /></a></center>
+
<center><img src="https://static.igem.org/mediawiki/igem.org/7/70/Data_Img1.png" style="width:60%;" height:250px" border="0" /></a></center>
<p>(A)Schematic diagram of Cas9/RNA-mediated gene targeting.</p>
<p>(A)Schematic diagram of Cas9/RNA-mediated gene targeting.</p>
<p>(B)Schematic representation of EGFP-A chimeric RNA (chiRNA) binding to pEGFP-N1 plasmid through spacer sequence.</p>
<p>(B)Schematic representation of EGFP-A chimeric RNA (chiRNA) binding to pEGFP-N1 plasmid through spacer sequence.</p>

Latest revision as of 01:00, 28 September 2013

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd"> main

DATA

(A)Schematic diagram of Cas9/RNA-mediated gene targeting.

(B)Schematic representation of EGFP-A chimeric RNA (chiRNA) binding to pEGFP-N1 plasmid through spacer sequence.

    Shen B, Zhang J, Wu H, et al. Generation of gene-modified mice via Cas9/ RNA mediated gene targeting[J]. Cell research, 2013.)



Data for Our Favorite New Parts:

1.BBa_K1160000-coding sequence of Cas9(from Type II CRISPR system):

  The coding sequence of cas9 derived from Streptococcus pyogenes (UniProtKB AC Num.:Q99ZW2), guided by crRNA and tracrRNA to complete its specific DNA cleavage function

2.BBa_K1160001- chimeric RNA:

  Combining crRNA and tracrRNA, designed to target the BBa_K1160002 part. Flanked by BsaA I site, which is replaceable for the desired editing sequence.

3.BBa_K1160002- cas9 cleavage detector:

  Contains an EGFP coding sequence and a crRNA targeting sequence, used to detect the specificity of cleavage activity conducted by Cas9 (BBa_K1160000) and chimeric RNA (BBa_K1160001)

Data for Our Favorite New Parts:

1. BBa_E2020- enhanced version of ECFP, yeast-optimized

2. BBa_E2030- enhanced version of EYFP, yeast-optimized


Retrieved from "http://2013.igem.org/Team:NJU_NJUT_China/data"