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Team:NTNU-Trondheim/3 July 2013
From 2013.igem.org
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| + | We isolated the DNA from the transformed cells (with RBS, pBAD and pTet) by using the [https://2013.igem.org/Team:NTNU-Trondheim/Protocols miniprep protocol] and measured the concentration by [http://www.nanodrop.com/library/nd-1000-v3.7-users-manual-8.5x11.pdf NanoDrop ND-1000 Spectrophotometer]. | ||
| + | |||
| + | {|border="1" | ||
| + | |Sample | ||
| + | |Concentration (ng/µl) | ||
| + | |- | ||
| + | |RBS | ||
| + | |4,91 | ||
| + | |- | ||
| + | |pTet1 | ||
| + | |8,99 | ||
| + | |- | ||
| + | |pTet2 | ||
| + | |4,39 | ||
| + | |- | ||
| + | |pBAD1 | ||
| + | |3,54 | ||
| + | |- | ||
| + | |pBAD2 | ||
| + | |9,24 | ||
|} | |} | ||
Latest revision as of 10:44, 4 July 2013
Wednesday 03.07.2013
The small-scale vesicle preparation continued with completion of step 2-7 in the vesicle isolation protocol. There where made three samples in step 2; E.coli ER2566 in LB, E.coli BW27784 in LB with ampenicillin (100 µg/mL) (ara-) and E.coli BW27784 in LB with ampenicillin (100 µg/mL) with the addition of arabinose (0.5 %) after 1 hour of incubtion (ara+).
Optical denisty (OD) at 600 nm was mesured as indicated in the table below.
| Sample | OD600 | Estimated CFU |
| ER2566 | 0.9805 | |
| ara- | 0.1263 | |
| ara+ | 0.0471 |
We isolated the DNA from the transformed cells (with RBS, pBAD and pTet) by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
| Sample | Concentration (ng/µl) |
| RBS | 4,91 |
| pTet1 | 8,99 |
| pTet2 | 4,39 |
| pBAD1 | 3,54 |
| pBAD2 | 9,24 |
