Team:NTNU-Trondheim/3 July 2013
From 2013.igem.org
Wednesday 03.07.2013
The small-scale vesicle preparation continued with completion of step 2-7 in the vesicle isolation protocol. There where made three samples in step 2; E.coli ER2566 in LB, E.coli BW27784 in LB with ampenicillin (100 µg/mL) (ara-) and E.coli BW27784 in LB with ampenicillin (100 µg/mL) with the addition of arabinose (0.5 %) after 1 hour of incubtion (ara+).
Optical denisty (OD) at 600 nm was mesured as indicated in the table below.
Sample | OD600 | Estimated CFU |
ER2566 | 0.9805 | |
ara- | 0.1263 | |
ara+ | 0.0471 |