Team:NTNU-Trondheim/3 July 2013


Wednesday 03.07.2013

The small-scale vesicle preparation continued with completion of step 2-7 in the vesicle isolation protocol. There where made three samples in step 2; E.coli ER2566 in LB, E.coli BW27784 in LB with ampenicillin (100 µg/mL) (ara-) and E.coli BW27784 in LB with ampenicillin (100 µg/mL) with the addition of arabinose (0.5 %) after 1 hour of incubtion (ara+).

Optical denisty (OD) at 600 nm was mesured as indicated in the table below.

Sample OD600 Estimated CFU
ER2566 0.9805
ara- 0.1263
ara+ 0.0471

We isolated the DNA from the transformed cells (with RBS, pBAD and pTet) by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.

Sample Concentration (ng/µl)
RBS 4,91
pTet1 8,99
pTet2 4,39
pBAD1 3,54
pBAD2 9,24