Notebook
Transformed BioBricks
Tuesday 18.06.13
Started transforming BioBricks. Transformed some composite BioBricks consisting of constitutive promotor, RBS, a fluorescent protein and a terminator. Transformed the parts Promoter+RBS+RFP+term (<partinfo>BBa_I13521</partinfo>), Promoter+RBS+GFP+term (<partinfo>BBa_I13522</partinfo>), Promoter+RBS+CFP+term (<partinfo>BBa_I13600</partinfo>) and Pm+RBS+mCherry+term to competent E.coli DH5α cells, to have some colorful cells to show the camera crew from NRK (Norwegian broadcasting), who will visit our lab tomorrow :-)
For our transformation protocol, see the protocol page.
Wednesday 19.06.13
We transformed BioBricks consisting of fluorescent proteins. Transformed the parts YFP (<partinfo>BBa_E0030</partinfo>), CFP (<partinfo>BBa_E0020</partinfo>), RFP (<partinfo>BBa_E1010</partinfo>), GFP (<partinfo>BBa_E0040</partinfo>), BFP (<partinfo>BBa_K592100</partinfo>) and SYFP (<partinfo>BBa_K864100</partinfo>).
We transferred mCherry from agar plate to liquid medium and incubated at 37° C.
Thursday 20.06.13
We transferred the transformed cells from yesterday to liquid medium and incubated at 37°C protocol.
We isolated the DNA from the transformed mCherry cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
| Sample
| Concentration (ng/µl)
|
| mCherry1
| 5,6
|
| mCherry2
| 7,3
|
Friday 21.06.13
We isolated the DNA from the transformed cells by using the miniprep protocol and measured the concentration by NanoDrop ND-1000 Spectrophotometer.
| Sample
| Concentration (ng/µl)
|
| CFP1
| 8,59
|
| CFP2
| 7,04
|
| BFP
| 7,06
|
| SYFP
| 5,42
|
| RFP1
| 5,04
|
| RFP2
| 7,52
|
| YFP
| 6,79
|
| GFP
| 5,27
|
Monday 24.06.13 - Friday 28.06.13
This week we have established protocols for further work and designed primers for PCR.
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