Team:NTNU-Trondheim/Primers

From 2013.igem.org

(Difference between revisions)
Line 174: Line 174:
<tr>
<tr>
<td><b>F_l_YFP</tr></b></td>
<td><b>F_l_YFP</tr></b></td>
-
<td></td>
+
<td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_YFP</tr></b></td>
<td><b>R_pl.b_YFP</tr></b></td>
-
<td></td>
+
<td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td>
</tr>
</tr>
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<tr>
<tr>
<td><b>F_pl.b_PrG</tr></b></td>
<td><b>F_pl.b_PrG</tr></b></td>
-
<td></td>
+
<td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_tat_PrG</tr></b></td>
<td><b>F_tat_PrG</tr></b></td>
-
<td></td>
+
<td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_pl.b_pelB_PrB</tr></b></td>
<td><b>F_pl.b_pelB_PrB</tr></b></td>
-
<td></td>
+
<td>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCgttgactcaccaatcgaagatacccc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_PrG</tr></b></td>
<td><b>R_pl.b_PrG</tr></b></td>
-
<td></td>
+
<td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b_PrGstop</tr></b></td>
<td><b>R_pl.b_PrGstop</tr></b></td>
-
<td></td>
+
<td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td>
</tr>
</tr>
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<tr>
<tr>
<td><b>F_plasmid </tr></b></td>
<td><b>F_plasmid </tr></b></td>
-
<td></td>
+
<td>5'-ccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_RFP_pl.b</tr></b></td>
<td><b>F_RFP_pl.b</tr></b></td>
-
<td></td>
+
<td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>F_YFP_pl.b</tr></b></td>
<td><b>F_YFP_pl.b</tr></b></td>
-
<td></td>
+
<td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_pl.b</tr></b></td>
<td><b>R_pl.b</tr></b></td>
-
<td></td>
+
<td>5'- ctagtatttctcctctttctctagtagtgc-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_tat_pl.b</tr></b></td>
<td><b>R_tat_pl.b</tr></b></td>
-
<td></td>
+
<td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td>
</tr>
</tr>
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<tr>
<tr>
<td><b>F_sequence</tr></b></td>
<td><b>F_sequence</tr></b></td>
-
<td></td>
+
<td>5'-tgccacctgacgtctaagaa-3'</td>
</tr>
</tr>
<tr>
<tr>
<td><b>R_sequence</tr></b></td>
<td><b>R_sequence</tr></b></td>
-
<td></td>
+
<td>5'-attaccgcctttgagtgagc-3'</td>
</tr>
</tr>

Revision as of 19:31, 4 October 2013

Trondheim iGEM 2013

header
Mercury
Primers



tat-signal sequence
Primer Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'
R_pl.b_YFP
5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'
Protein G
F_pl.b_PrG
5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'
F_tat_PrG
5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'
F_pl.b_pelB_PrB
5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCgttgactcaccaatcgaagatacccc-3'
R_pl.b_PrG
5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'
R_pl.b_PrGstop
5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'
Plasmid backbone
F_plasmid
5'-ccaggcatcaaataaaacgaaaggc-3'
F_RFP_pl.b
5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'
F_YFP_pl.b
5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'
R_pl.b
5'- ctagtatttctcctctttctctagtagtgc-3'
R_tat_pl.b
5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'
Sequencing Primers
F_sequence
5'-tgccacctgacgtctaagaa-3'
R_sequence
5'-attaccgcctttgagtgagc-3'