Team:NTNU-Trondheim/Primers

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Trondheim iGEM 2013

header
Mercury
Primers



tat-signal sequence
Primer Sequence
F_pl.b_tat
5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3'
F_prefix_tat
5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3'
R_tat
5'-cgcttgcgccgcagtcgcacgtcg-3'
R_GFP_tat
5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3'
R_CFP_tat
5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3'
SYFP
F_I_syfp
5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3'
R_syfp
5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3'
BFP
F_tat_BFP
5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3'
R_I_bfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3'
GFP
F_tat_GFP
5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3'
R_I_gfp
5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3'
RFP
F_I_rfp
5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3'
R_pl.b_rfp
5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3'
GFPmut3
F_tat_GFPmut3
5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3'
R_pl.b_GFPmut3
5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3'
CFP
F_tat_CFP
5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3'
R_l_CFP
5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3'
YFP
F_l_YFP
R_pl.b_YFP
Protein G
F_pl.b_PrG
F_tat_PrG
F_pl.b_pelB_PrB
R_pl.b_PrG
R_pl.b_PrGstop
Plasmid backbone
F_plasmid
F_RFP_pl.b
F_YFP_pl.b
R_pl.b
R_tat_pl.b
Sequencing Primers
F_sequence
R_sequence