Team:NTNU-Trondheim/Protocols

From 2013.igem.org

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== Protocols ==
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== Laboratory Protocols ==
====Transformation====
====Transformation====
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Official iGEM [http://parts.igem.org/Help:Protocols/Transformation Transformation protocol].
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=====Materials=====
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*Resuspended DNA (''Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes'')
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*[[Help:Protocols/Competent_Cells | Competent cells]] (''50ul per transformation'')
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*Ice (in ice bucket/container)
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*2ml tube (''1 per a transformation''')
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*42ºC water bath
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*SOC media (''check for contamination!'')
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*Petri dishes with LB agar and appropriate antibiotic (''2 per transformation'')
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*glass beads or spreader
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*37ºC incubator
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*10pg/ul RFP Control (pSB1A3 w/ BBa_J04450)
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== more protocols ==
== more protocols ==

Revision as of 08:57, 21 June 2013

iGEM 2013
NTNU Trondheim
iGEM NTNU 2013




Laboratory Protocols

Transformation

Official iGEM Transformation protocol.

more protocols


Retrieved from "http://2013.igem.org/Team:NTNU-Trondheim/Protocols"