Team:NTNU-Trondheim/Protocols

Laboratory Protocols

Transformation

Official iGEM Transformation protocol.

We used this protocol with these modifications:

• Heat-shock 45 seconds instead of 60 seconds
• Incubation for min 1 hour with shaking at 37°C, not 2 hours.
• LB medium instead of SOC.

Transformed cells

Use a sterile toothpick, scratch a single colony from the transformed cells. Place the toothpick into a plastic tube with 4 mL sterile liquid medium with the appropriate antibiotic(s). Cap the tube and incuate at 37°C with shaking.

DNA Isolation

We use the Promega Wizard Plus SV Minipreps DNA Purification System A1460 Miniprep protocol.

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