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Team:NTNU-Trondheim/Protocols
From 2013.igem.org
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(→Laboratory Protocols) |
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*Incubation for min 1 hour in shaker at 37°C, not 2 hours. | *Incubation for min 1 hour in shaker at 37°C, not 2 hours. | ||
*LB medium instead of SOC. | *LB medium instead of SOC. | ||
| + | |||
| + | ====Transformed cells==== | ||
| + | Use a sterile toothpick, scratch a single colony from the transformed cells. Place the toothpick into a plastic tube with 4 mL sterile liquid medium with the appropriate antibiotic(s). Cap the tube and incuate at 37°C with shaking. | ||
====DNA Isolation==== | ====DNA Isolation==== | ||
Revision as of 09:28, 21 June 2013
