Team:NTNU-Trondheim/Protocols

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Contents

1 Protocols
- 1.1 Transformation
  - 1.1.1 Materials
2 more protocols

Protocols

Transformation

Materials

Resuspended DNA (Resuspend well in 10ul dH20, pipette up and down several times, let sit for a few minutes)
Competent cells (50ul per transformation)
Ice (in ice bucket/container)
2ml tube (1 per a transformation')
42ºC water bath
SOC media (check for contamination!)
Petri dishes with LB agar and appropriate antibiotic (2 per transformation)
glass beads or spreader
37ºC incubator
10pg/ul RFP Control (pSB1A3 w/ BBa_J04450)

more protocols

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