Team:NTNU-Trondheim/Protocols

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Contents

1 Laboratory Protocols
- 1.1 Transformation
- 1.2 DNA Isolation
2 more protocols

Laboratory Protocols

Transformation

Official iGEM Transformation protocol.

We used this protocol with these modifications:

Heat-shock 45 seconds instead of 60 seconds
Incubation for min 1 hour in shaker at 37°C, not 2 hours.
LB medium instead of SOC.

DNA Isolation

We use the Promega Wizard Plus SV Minipreps DNA Purification System A1460 Miniprep protocol.

more protocols

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