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Official iGEM Transformation protocol.
We used this protocol with these modifications:
Use a sterile toothpick, scratch a single colony from the transformed cells. Place the toothpick into a plastic tube with 4 mL sterile liquid medium with the appropriate antibiotic(s). Cap the tube and incuate at 37°C with shaking.
We use the Promega Wizard Plus SV Minipreps DNA Purification System A1460 Miniprep protocol.