Team:NTU-Taida/Parts

From 2013.igem.org

Part



Contents

Note

Some plasmids are not submitted due to similar parts in registry or time limit while they are still important fragments in our projects and we still lists them below.

Also, BBa_K575024 and BBa_K575033 submitted from the Northwestern help a lot.

Original Biobrick

NameTypeDescriptionLength
BBa_K1157000Regulatorypromoter sequence for PQS quorum sensing341
BBa_K1157001CodingPQSR999
BBa_K1157003CodingAbaR597

Reporter

NameTypeDescriptionLength
BBa_K1157005ReporterLuciferase generator1094
 ReporterRBS-mTagBFP-tt849
 ReporterRBS-mCherry-tt858
 ReporterRBS-GFP-tt867

Protein Generator

NameTypeDescriptionLength
BBa_K1157007CodingQuorum sensing receptor pqsR generator1157
BBa_K1157017CompositepPQS-GFPmut31227
BBa_K1157019CompositePc-PQSR1200
 CompositeRBS-RhlR-tt873
 CompositeRBS-LasR-tt867
 CompositeRBS-CinR-tt931
 CompositeRBS-AbaR-tt741

Without positive feedback circuits

NameTypeDescriptionLength
BBa_K1157010CompositePc-RhlR-mCherry1871
BBa_K1157016CompositePc-LasR-pLas-BFP1960
BBa_K575033CompositePRhl-GFP-Pc-RhlR1600
BBa_K575024CompositePlas-GFP-Pc-LasR1701
BBa_K1157008CompositepLas-BFP-Pc-LasR2018
 CompositepPQS-GFP-Pc-PQSR2401

Positive Feedback circuit

NameTypeDescriptionLength
BBa_K1157020CompositePc-RhlR-pRhl-RhlR-GFPmut32635
BBa_K1157011CompositePc-RhlR-pRhl-RhlR-BFP2620
BBa_K1157012CompositePc-RhlR-pRhl-RhlR-luciferase2851
BBa_K1157018CompositepRhl-RhlR-mCherry-Pc-RhlR2629
BBa_K1157021CompositepRhl-RhlR-GFP-Pc-RhlR2635
BBa_K1157014CompositepLas-LasR-GFPmut3-Pc-LasR2727

Negative Regulation

NameTypeDescriptionLength
BBa_K1157013CompositepCI-mCherry-pConst-RhlR-pRhl-RhlR-CI3627
BBa_K1157015CompositepConst-Rhl-pRhl-RhlR-CI-pCI-mCherry3627