Team:NTU Taiwan/index.html

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         <br/><p><b>PCR</b></p><br/>
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         <br/><p><b>PCR</b></p>
1: Design of appropriate forward and reverse primers<br/>
1: Design of appropriate forward and reverse primers<br/>
  2: Prepare our template<br/>
  2: Prepare our template<br/>
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  4: Run PCR<br/>
  4: Run PCR<br/>
  5: Examine the results by electrophoresis<br/>
  5: Examine the results by electrophoresis<br/>
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         Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS
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         Note: If the template is genomic DNA, we would adjust the annealing temperature at 45°C. It is because the copy number of target gene may be low. We use this annealing temp when perform PCR of Tir1, 26s, 5.8s ITS<br/><br/>
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<br/><p><b>Construction of our parts</b></p><br/><p>
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<br/><p><b>Construction of our parts</b></p><p>
  1: We design primers for parts with prefix and suffix.<br/>
  1: We design primers for parts with prefix and suffix.<br/>
2: Perform PCR and cleanup the PCR product<br/>
2: Perform PCR and cleanup the PCR product<br/>

Latest revision as of 04:22, 28 September 2013

iGEM-NTU-Taiwan