Team:NU Kazakhstan/Aptamers

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<h3><center>Selection of ssDNA Aptamers for Use in Detection of Cancer Biomarker-Carcinoembryonic Antigen</center></h3>
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<div>1. <b>PCR AMPLIFICATION CYCLE AND PRIMER CONCENTRATION OPTIMIZATION EXPERIMENTS</b></div>
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<div><u>PCR optimization conditions: our reagent and Qiagen reagents</u></div>
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<p>Since PCR products, that were obtained using combination of our own reagents (Taq.pol from New England Biolabs, MgCl2 from NCB and so on), were faint on gel I decided to use Qiagen PCR amplification kit, which I found in the freezer. I run two reactions at the same. As the result bands for PCR products from Qiagen were more visible and stronger.</p>
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<br/><img src="https://static.igem.org/mediawiki/2013/4/4b/Selex_1.png" alt="" />
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<p>Conclusion: use Qiagen reagent kit for further PCR amplifications</p>
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<p>Problem: still having multiple bands and smears</p>
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Revision as of 18:04, 25 September 2013

NU_Kazakhstan

Selection of ssDNA Aptamers for Use in Detection of Cancer Biomarker-Carcinoembryonic Antigen

1. PCR AMPLIFICATION CYCLE AND PRIMER CONCENTRATION OPTIMIZATION EXPERIMENTS
PCR optimization conditions: our reagent and Qiagen reagents

Since PCR products, that were obtained using combination of our own reagents (Taq.pol from New England Biolabs, MgCl2 from NCB and so on), were faint on gel I decided to use Qiagen PCR amplification kit, which I found in the freezer. I run two reactions at the same. As the result bands for PCR products from Qiagen were more visible and stronger.


Conclusion: use Qiagen reagent kit for further PCR amplifications

Problem: still having multiple bands and smears