http://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/abstract&feed=atom&action=historyTeam:Nanjing-China/abstract - Revision history2024-03-28T22:32:06ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/abstract&diff=335181&oldid=prevGaoJian NJU at 06:18, 27 October 20132013-10-27T06:18:17Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Atrazine, a <del class="diffchange diffchange-inline">widely used herbicide, persists for a long period </del>in the <del class="diffchange diffchange-inline">environment onced used</del>. <del class="diffchange diffchange-inline">It </del>causes metabolic disorders in <del class="diffchange diffchange-inline">both animals and humankind</del>. Our <del class="diffchange diffchange-inline">team utilized the ribosome switch induced by </del>atrazine, a <del class="diffchange diffchange-inline">QS </del>system <del class="diffchange diffchange-inline">of Plux </del>and a degrading enzyme to <del class="diffchange diffchange-inline">control E.coil's motility through regulating its CheZ gene</del>. <del class="diffchange diffchange-inline">Therefore</del>, E.coli can recognize atrazine, recruit team <del class="diffchange diffchange-inline">workers</del>, and <del class="diffchange diffchange-inline">degrade </del>atrazine. Our team found <del class="diffchange diffchange-inline">a </del>transporter of atrazine, <del class="diffchange diffchange-inline">which we call TRM</del>. We also mutated the degrading enzyme, TrzN, <del class="diffchange diffchange-inline">making </del>it better at <del class="diffchange diffchange-inline">degradation</del>. We combined TRM and the TrzN to <del class="diffchange diffchange-inline">improve atrazine absorbance and degradation</del>. <del class="diffchange diffchange-inline">Moreover</del>, our team <del class="diffchange diffchange-inline">are trying </del>to <del class="diffchange diffchange-inline">analyze </del>and <del class="diffchange diffchange-inline">compare </del>several systems <del class="diffchange diffchange-inline">with </del>computer, <del class="diffchange diffchange-inline">hoping to find </del>the best <del class="diffchange diffchange-inline">one </del>which is equipped with faster moving and quicker degrading. Overall,we believe our system <del class="diffchange diffchange-inline">will boost the industrialization</del>, <del class="diffchange diffchange-inline">universalization as well as standardization in the field of treatment </del>for <del class="diffchange diffchange-inline">atrazine and other versatile small molecules</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Atrazine, a <ins class="diffchange diffchange-inline">naughty demon who plays around </ins>in the <ins class="diffchange diffchange-inline">soil, is considerably hard to be eliminated</ins>. <ins class="diffchange diffchange-inline">Its spell </ins>causes metabolic disorders in <ins class="diffchange diffchange-inline">many species</ins>.<ins class="diffchange diffchange-inline"><br><br> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our <ins class="diffchange diffchange-inline">elf combined a smart </ins>atrazine <ins class="diffchange diffchange-inline">radar, an amazing transporter</ins>, a <ins class="diffchange diffchange-inline">recruiting </ins>system and a degrading enzyme to <ins class="diffchange diffchange-inline">hunt down this die-hard</ins>. <ins class="diffchange diffchange-inline">With our system</ins>, E.coli can recognize atrazine, recruit team <ins class="diffchange diffchange-inline">members</ins>, and <ins class="diffchange diffchange-inline">eliminate </ins>atrazine.<ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team found <ins class="diffchange diffchange-inline">the brand new </ins>transporter<ins class="diffchange diffchange-inline">, TRM, from the genome </ins>of <ins class="diffchange diffchange-inline">a bacteria which can utilize </ins>atrazine <ins class="diffchange diffchange-inline">as its only carbon source. With this transporter</ins>, <ins class="diffchange diffchange-inline">the sensitivity of our system and the degrading efficiency are both improved</ins>. We also mutated the degrading enzyme, TrzN, <ins class="diffchange diffchange-inline">to make </ins>it better at <ins class="diffchange diffchange-inline">degrading atrazine than the natural enzyme</ins>. We <ins class="diffchange diffchange-inline">then </ins>combined <ins class="diffchange diffchange-inline">the </ins>TRM and the <ins class="diffchange diffchange-inline">mutated </ins>TrzN to <ins class="diffchange diffchange-inline">achieve a more satisfying result</ins>.<ins class="diffchange diffchange-inline"><br><br> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">To take a further step</ins>, our team <ins class="diffchange diffchange-inline">has been devoting ourselves </ins>to <ins class="diffchange diffchange-inline">analyzing </ins>and <ins class="diffchange diffchange-inline">comparing </ins>several systems <ins class="diffchange diffchange-inline">simulated by </ins>computer, <ins class="diffchange diffchange-inline">and we successfully located on </ins>the best <ins class="diffchange diffchange-inline">solution </ins>which is equipped with faster moving and quicker degrading.<ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Overall, we believe <ins class="diffchange diffchange-inline">that </ins>our system <ins class="diffchange diffchange-inline">has provided a brilliant solution to make this world a better place for creatures on this planet</ins>, <ins class="diffchange diffchange-inline">hoping it can also become a sincere compensation </ins>for <ins class="diffchange diffchange-inline">the damage resulting from this naughty demon created by ourselves</ins>.<ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
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</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/abstract&diff=209297&oldid=prevUltramagnuslvkun at 15:06, 27 September 20132013-09-27T15:06:18Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Atrazine, a widely used herbicide, persists for a long period in the environment onced used. It causes metabolic disorders in both animals and humankind. Our team utilized the ribosome switch induced by atrazine, a QS system of Plux and a degrading enzyme to control E.coil's motility through regulating <del class="diffchange diffchange-inline">it's </del>CheZ gene. Therefore, E.coli can recognize atrazine, recruit team workers, and degrade atrazine. Our team found a transporter of atrazine, which we call TRM. We also mutated the degrading enzyme, TrzN, making it better at degradation. We combined TRM and the TrzN to improve atrazine absorbance and degradation. Moreover, our team are trying to analyze and compare several systems with computer, hoping to find the best one which is equipped with faster moving and quicker degrading. Overall,we believe our system will boost the industrialization, universalization as well as standardization in the field of treatment for atrazine and other versatile small molecules. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Atrazine, a widely used herbicide, persists for a long period in the environment onced used. It causes metabolic disorders in both animals and humankind. Our team utilized the ribosome switch induced by atrazine, a QS system of Plux and a degrading enzyme to control E.coil's motility through regulating <ins class="diffchange diffchange-inline">its </ins>CheZ gene. Therefore, E.coli can recognize atrazine, recruit team workers, and degrade atrazine. Our team found a transporter of atrazine, which we call TRM. We also mutated the degrading enzyme, TrzN, making it better at degradation. We combined TRM and the TrzN to improve atrazine absorbance and degradation. Moreover, our team are trying to analyze and compare several systems with computer, hoping to find the best one which is equipped with faster moving and quicker degrading. Overall,we believe our system will boost the industrialization, universalization as well as standardization in the field of treatment for atrazine and other versatile small molecules. </div></td></tr>
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</table>Ultramagnuslvkunhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/abstract&diff=165126&oldid=prevGaoJian NJU at 03:27, 25 September 20132013-09-25T03:27:26Z<p></p>
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</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/abstract&diff=158636&oldid=prevGaoJian NJU at 08:24, 24 September 20132013-09-24T08:24:49Z<p></p>
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Atrazine, a widely used herbicide, persists for a long period in the environment onced used. It causes metabolic disorders in both animals and humankind. Our team utilized the ribosome switch induced by atrazine, a QS system of Plux and a degrading enzyme to control E.coil's motility through regulating it's CheZ gene. Therefore, E.coli can recognize atrazine, recruit team workers, and degrade atrazine. Our team found a transporter of atrazine, which we call TRM. We also mutated the degrading enzyme, TrzN, making it better at degradation. We combined TRM and the TrzN to improve atrazine absorbance and degradation. Moreover, our team are trying to analyze and compare several systems with computer, hoping to find the best one which is equipped with faster moving and quicker degrading. Overall,we believe our system will boost the industrialization, universalization as well as standardization in the field of treatment for atrazine and other versatile small molecules. <br />
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