http://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&feed=atom&action=historyTeam:Nanjing-China/rib - Revision history2024-03-29T09:45:38ZRevision history for this page on the wikiMediaWiki 1.16.5http://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=334635&oldid=prevGaoJian NJU at 03:04, 27 October 20132013-10-27T03:04:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine (Fig. 3-4-3).. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine (Fig. 3-4-3).. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="600"></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="600"></p><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before<ins class="diffchange diffchange-inline">. <br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">However, for the reason of fluorescence quenching, the fluorescence intensity would descending for too high concentration of GFP. So we detected the RFU changing with time and atrazine’s concentration and identified the range of concentration of atrazine (Fig. 3-4-4)</ins>. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/2/2f/3-4-4.jpg"></p><br/></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-4</strong> The RFU changing with time and atrazine’s concentration.</div></ins><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td></tr>
</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=221919&oldid=prevSquid at 21:14, 27 September 20132013-09-27T21:14:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="600"></p><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="600"></p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.</div><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.</div><br/><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine <ins class="diffchange diffchange-inline">(Fig. 3-4-3).</ins>. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="600"></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="600"></p><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td></tr>
</table>Squidhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=221879&oldid=prevSquid at 21:13, 27 September 20132013-09-27T21:13:53Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch created via a new method to detect atrazine <sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch created via a new method to detect atrazine <sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <del class="diffchange diffchange-inline"><strong></del>(Fig. 3-4-1)<del class="diffchange diffchange-inline"></strong></del>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine (Fig. 3-4-1). This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="600"></p><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center"><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="600"></p><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td></tr>
</table>Squidhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=221847&oldid=prevSquid at 21:13, 27 September 20132013-09-27T21:13:05Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="<del class="diffchange diffchange-inline">716</del>"><<del class="diffchange diffchange-inline">br</del>/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p style="text-align:center"></ins><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="<ins class="diffchange diffchange-inline">600</ins>"></<ins class="diffchange diffchange-inline">p</ins>><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="<del class="diffchange diffchange-inline">716</del>"><<del class="diffchange diffchange-inline">br</del>><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p style="text-align:center"></ins><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="<ins class="diffchange diffchange-inline">600</ins>"><<ins class="diffchange diffchange-inline">/p</ins>><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.</div><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.</div><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="<del class="diffchange diffchange-inline">716</del>"><<del class="diffchange diffchange-inline">br</del>/><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p style="text-align:center"></ins><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="<ins class="diffchange diffchange-inline">600</ins>"></<ins class="diffchange diffchange-inline">p</ins>><br<ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the expression of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td></tr>
</table>Squidhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=210472&oldid=prevUltramagnuslvkun at 15:47, 27 September 20132013-09-27T15:47:23Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch created via a new method to detect atrazine <sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch created via a new method to detect atrazine <sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Our </del>team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Our </ins>team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="716"><br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7a/Fig_3-4-1.jpg" width="716"><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> Our </del>team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Our </ins>team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td></tr>
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</table>Ultramagnuslvkunhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=210434&oldid=prevUltramagnuslvkun at 15:46, 27 September 20132013-09-27T15:46:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <dd class="dd_1"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <dd class="dd_1"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch <del class="diffchange diffchange-inline">which can detect atrazine </del>created via a new method <sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Ribosome switch is used widely in synthetic biology to detect various small molecules. This summer, we chose an efficient ribosome switch created via a new method <ins class="diffchange diffchange-inline">to detect atrazine </ins><sup>[1]</sup>. And then, our team verified the basic parameters about this amazing ribosome switch, including the lowest detect concentration and the induced time.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Experiments and Results</strong><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Our team used this kind of ribosome switch which can detect atrazine to control the GFP expression in E.coli. E.coli expressed GFP after 20 hours under 500μM of atrazine, but could not express GFP without atrazine <strong>(Fig. 3-4-1)</strong>. This shows the ribosome switch can be turned on under 500μM of atrazine.<br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the <del class="diffchange diffchange-inline">express </del>of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the <ins class="diffchange diffchange-inline">expression </ins>of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td></tr>
</table>Ultramagnuslvkunhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=209432&oldid=prevGaoJian NJU at 15:10, 27 September 20132013-09-27T15:10:39Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class="bar">Project</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class="bar">Project</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/project">Overall Project</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/project">Overall Project</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/pre">Pre-<del class="diffchange diffchange-inline">experiment</del></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/pre">Pre-<ins class="diffchange diffchange-inline">experiments</ins></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/tran">Transportation</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/tran">Transportation</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/rib" class="son_hover">Recognition</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/rib" class="son_hover">Recognition</a></li></div></td></tr>
</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=205535&oldid=prevGaoJian NJU at 12:32, 27 September 20132013-09-27T12:32:41Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.<ins class="diffchange diffchange-inline"></div><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<del class="diffchange diffchange-inline"></div></del><br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the express of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="padding:0 50px; font-size:11px"><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the express of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.</div><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <ins class="diffchange diffchange-inline"><br/></ins><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td></tr>
</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=205332&oldid=prevGaoJian NJU at 12:23, 27 September 20132013-09-27T12:23:34Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div style="padding:0 50px; font-size:11px"></ins><strong>Fig. 3-4-1</strong> The ribosome switch exhibits conformations and function. (A) The principle of how the ribosome switch works. (B) Ribosome switch is induced by atrazine under 500μM of atrazine.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> Our team used this part to control GFP expression in E.coli. We found all E.coli had expressed GFP under all the concentrations of atrazine, from 1μM to 500μM, in our experiment (Fig. 3-4-2). This data indicates that the ribosome switch can work under 1μM of atrazine.<ins class="diffchange diffchange-inline"></div></ins><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/0/04/Fig_3-4-2.jpg" width="716"><br><br></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.<br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div style="padding:0 50px; font-size:11px"></ins><strong>Fig. 3-4-2</strong> Various concentrations of atrazine were detected after 20h culturing. We detect the expression of GFP after the strain K-12 is cultured for 20h in the different concentrations from 1μM to 500μM.<ins class="diffchange diffchange-inline"></div></ins><br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our team used this part to control the GFP expression in E.coli. We found E.coli had merely expressed GFP after 12 hours under 200μM of atrazine. This data elucidates that the ribosome switch can work after 12 hours under 200μM of atrazine.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="716"><br/><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2013/7/7d/Fig_3-4-3.jpg" width="716"><br/><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the express of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.<br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><div style="padding:0 50px; font-size:11px"></ins><strong>Fig. 3-4-3</strong> The ribosome switch was induced at different time under 200μM of atrazine. We detect the express of GFP after that the strain K-12 is cultured under 200μM of atrazine at the different time from 6h to 12h.<ins class="diffchange diffchange-inline"></div></ins><br><br>According to these data, this ribosome switch is more efficient than the one used before. Therefore, we are confident of the fact that we can put this amazing part into detecting atrazine in real life. <br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><strong>Reference</strong><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[1] Sinha J, et al. Reprogramming bacteria to seek and destroy a herbicide. Nature chemical biology, 2010, 6 (6): 464-470. </div></td></tr>
</table>GaoJian NJUhttp://2013.igem.org/wiki/index.php?title=Team:Nanjing-China/rib&diff=200549&oldid=prevGaoJian NJU at 07:42, 27 September 20132013-09-27T07:42:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/pre">Pre-experiment</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/pre">Pre-experiment</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/tran">Transportation</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/tran">Transportation</a></li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/qs">Aggregation</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li class=trunk onmouseover=listTrigger(0);><a href="https://2013.igem.org/Team:Nanjing-China/qs">Aggregation</a></li></div></td></tr>
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</table>GaoJian NJU