Team:Nanjing-China/text

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                <p>Nanjing-China 2012 is working on small interfering genetically engineered machine.</p>
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                  <p>Our team</p>
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                  <p>Engineered lettuce growing</p>
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          <h2>siGEM at a glance</h2>
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          <p class="MsoNormal"><span lang="EN-US">The essential and expanding roles of
 +
small non coding RNAs are becoming clearer to us. More interestingly, plant
 +
endogenous small RNAs through food intake directly influence gene expression in
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animals (especially in liver) , as a recent study showed.&nbsp;&nbsp; </span></p>
 +
<p class="MsoNormal"><span lang="EN-US">We took advantage of this
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<span class="word">phenomenon</span>, and made engineered lettuce producing
 +
artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha,
 +
whose high expression in liver can cause insulin resistance, as target. (More...
 +
in project section)</span></p>          </div>
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          <h2>Parts at a glance</h2>
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          <p class="MsoNormal"><span lang="EN-US">Our team designed three main modules for
 +
our project, si/mi RNA and green plant synthetic biology. They may serve as a
 +
bit of ground work for future teams working on plant.</span></p>
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<p class="MsoNormal"><span lang="EN-US">They are RNA generating module, T-DNA
 +
module, and Barcode 2 module. </span></p>
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<p class="MsoNormal"><span lang="EN-US">&nbsp;RNA generating module make vegetable a
 +
small RNA producing factory.</span></p>
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<p class="MsoNormal"><span lang="EN-US">Parts in T-DNA module are used for
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constructing customized binary vector for plant.</span></p>
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<p class="MsoNormal"><span lang="EN-US">Barcode 2 module is a signature system
 +
for engineered plant.</span></p>
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<p class="MsoNormal"><span lang="EN-US">&nbsp;</span></p>
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<p class="MsoNormal"><span lang="EN-US">(Read more in Parts and project)</span></p>
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        <div class="bar"><span>企业文化</span><a href="/a/about"><img src="/templets/default/images/more.gif" /></a></div>
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          <p class="MsoNormal"><span lang="EN-US">You can simply use overlapping PCR to
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switch the sequence of small RNAs in part BBa_K788002 to make your own parts and
-
          <img src="/templets/default/images/1.jpg" width="210" height="140" />
+
plant. (Detail is available in the part registry
-
          <span>&nbsp;&nbsp;&nbsp;&nbsp;泰州市华通消防装备厂有限公司，消防同行业中龙头企业，镶嵌在长江三角洲苏中平原历史悠久、人文荟萃的泰州市，毗邻古城扬州，地理位置优越，处于京沪、宁通、宁盐公路交汇处，环境优美，水陆交通十分便利。是一家集产品科研、开发、生产、销售、服务于一体的现代化企业。<br />
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<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788002">
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　　公司始建于 1985 年，在董事长薛亚明先生等领导的带领下，历经 20 年的艰苦创业，公司现形成了占地面积4万平方米，总建筑面积2.8 万平方米的现代化厂房......</span>
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BBa_K788002</a>). Also, you can use part<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788010">  
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BBa_K788010 </a>and<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788005">
 +
BBa_K7880005</a> to make new binary vectors. And you may put the signature on
 +
the plant you work with by adding a barcode 2 part at the end of your system
 +
according to rules
 +
<a style="color: blue; text-decoration: underline; text-underline: single" href="http://partsregistry.org/Part:BBa_K788007">
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BBa_K880007</a> gave.</span></p>          </div>
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Revision as of 01:46, 23 September 2013

siGEM at a glance

The essential and expanding roles of small non coding RNAs are becoming clearer to us. More interestingly, plant endogenous small RNAs through food intake directly influence gene expression in animals (especially in liver) , as a recent study showed.  

We took advantage of this phenomenon, and made engineered lettuce producing artificial small RNA for therapeutic purpose. We in this case chose PGC1-alpha, whose high expression in liver can cause insulin resistance, as target. (More... in project section)

Parts at a glance

Our team designed three main modules for our project, si/mi RNA and green plant synthetic biology. They may serve as a bit of ground work for future teams working on plant.

They are RNA generating module, T-DNA module, and Barcode 2 module.

 RNA generating module make vegetable a small RNA producing factory.

Parts in T-DNA module are used for constructing customized binary vector for plant.

Barcode 2 module is a signature system for engineered plant.

 

(Read more in Parts and project)

Sharing

You can simply use overlapping PCR to switch the sequence of small RNAs in part BBa_K788002 to make your own parts and plant. (Detail is available in the part registry BBa_K788002). Also, you can use part BBa_K788010 and BBa_K7880005 to make new binary vectors. And you may put the signature on the plant you work with by adding a barcode 2 part at the end of your system according to rules BBa_K880007 gave.

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