Team:Nevada/Notebook/Month1
From 2013.igem.org
Contents |
May 20
The whole team met to discuss project goals, human practice ideas, and training concerns. We made preliminary plans for how to divide the work and exchanged contact information so that everyone could keep in touch.
May 21
The new team members met with Dr. Howard to learn the basics of making plates and antibiotic stocks, operating the autoclave, and doing dishes.
Plates with the Erwinia endolysin, Xanthomonas endolysin, and ice nuclease target sequence were streaked out.
May 22
The team met to go over pBAD cloning and expression protocols as well as primer design strategies.
We prepared liquid cultures of the Erwinia endolysin, Xanthomonas endolysin, and ice nuclease target sequence.