Team:Nevada/Protocols

From 2013.igem.org

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:::#To recover, scrape frozen surface of culture with sterile inoculating needle, and then streak onto LB agar plate containing appropriate antibiotics, or inoculate liquid culture containing appropriate antibiotics.
:::#To recover, scrape frozen surface of culture with sterile inoculating needle, and then streak onto LB agar plate containing appropriate antibiotics, or inoculate liquid culture containing appropriate antibiotics.
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==DNA Quantification using NanoDrop Spectrophotometry==
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==DNA Quantification using NanoDrop==
:::#Select ''Nucleic Acids'' measurement
:::#Select ''Nucleic Acids'' measurement
:::#Initialize the NanoDrop spectrophotometer with 2µL of autoclaved H2O and wipe off
:::#Initialize the NanoDrop spectrophotometer with 2µL of autoclaved H2O and wipe off
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:::#Measure 1.5µL of DNA sample and record the concentration in ng/µL
:::#Measure 1.5µL of DNA sample and record the concentration in ng/µL
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==Gel Purification of DNA(Qiagen QIAquick Gel Extraction Kit)==
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==Gel Purification of DNA==
:::#Cut out the DNA fragment from the agarose gel with a razor blade, while minimizing the size of the gel slice
:::#Cut out the DNA fragment from the agarose gel with a razor blade, while minimizing the size of the gel slice
:::#Weigh the gel slice and add 3 volumes of Buffer QG to every 1 volume of gel (100mg = 100µL)
:::#Weigh the gel slice and add 3 volumes of Buffer QG to every 1 volume of gel (100mg = 100µL)

Revision as of 06:31, 27 September 2013