Team:Nevada/Protocols

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(Difference between revisions)
(Created page with "{{:Team:Nevada/Templates/header}} ==Bacterial Glycerol Stocks== :::#Put 0.5mL bacterial culture in a sterile eppendorf tube. :::#Add 0.5mL of sterile 80% (v/v) glycerol solution...")
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:::#Measure 1.5µL of DNA sample and record the concentration in ng/µL
:::#Measure 1.5µL of DNA sample and record the concentration in ng/µL
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==Gel Purification of DNA ''(Qiagen QIAquick Gel Extraction Kit)''==
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==Gel Purification of DNA  
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''(Qiagen QIAquick Gel Extraction Kit)''==
:::#Cut out the DNA fragment from the agarose gel with a razor blade, while minimizing the size of the gel slice
:::#Cut out the DNA fragment from the agarose gel with a razor blade, while minimizing the size of the gel slice
:::#Weigh the gel slice and add 3 volumes of Buffer QG to every 1 volume of gel (100mg = 100µL)
:::#Weigh the gel slice and add 3 volumes of Buffer QG to every 1 volume of gel (100mg = 100µL)

Revision as of 06:29, 27 September 2013