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Team:Northwestern/makem9 - Revision history
2024-03-29T15:26:02Z
Revision history for this page on the wiki
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2013.igem.nu at 00:36, 28 September 2013
2013-09-28T00:36:56Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 00:36, 28 September 2013</td>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="https://2013.igem.org/Team:Northwestern/Notebook">Notebook</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <li><a href="#">Description</a></li></del></div></td><td colspan="2"> </td></tr>
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2013.igem.nu
http://2013.igem.org/wiki/index.php?title=Team:Northwestern/makem9&diff=227069&oldid=prev
2013.igem.nu: Created page with "{{:Team:Northwestern/Templates/Skinning}} <html> <style> p { color:black; font-family: helvetica; } .container { display: inline-block; background-color: #C3..."
2013-09-27T23:24:34Z
<p>Created page with "{{:Team:Northwestern/Templates/Skinning}} <html> <style> p { color:black; font-family: helvetica; } .container { display: inline-block; background-color: #C3..."</p>
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<li><a href="https://2013.igem.org/Team:Northwestern/Team">Team</a><br />
<ul><br />
<li><a href="https://igem.org/Team.cgi?year=2013&team_name=Northwestern">Official Profile</a></li><br />
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<h1> Make M9</h1><br />
<div><br />
<br />
<b>M9 minimal media - Store at 4C:</b><br />
<ul><br />
<li>1x M9 salts (From 5X autoclaved stock)</li><br />
<li>2mM MgSO4 (From 1M autoclaved stock) </li><br />
<li>0.1mM CaCl2 (From 1M autoclaved stock) </li><br />
<li>0.4% carbon source (From 40% glucose filtered stock) </li><br />
<li>0.25ug/ml Biotin (From filtered 1000X, 0.25mg/ml stock) - EcNR2-specific </li><br />
<li>0.00005% Vitamin B1 (thiamine) (From 10,000X, 0.5% filtered stock) - MG1655-specific (see Jensen, J. Bacteriol., 1993) </li><br />
</ul><br />
<p>Bring to volume with sterile water</p><br />
<b>5X M9 salts:</b><br />
<ul><br />
<li>64g Na2HPO4*7H2O</li><br />
<li>15g KH2PO4</li><br />
<li>2.5g NaCl</li><br />
<li>5g NH4Cl</li><br />
</ul><br />
</div></div>
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