Make M9

M9 minimal media - Store at 4C:
  • 1x M9 salts (From 5X autoclaved stock)
  • 2mM MgSO4 (From 1M autoclaved stock)
  • 0.1mM CaCl2 (From 1M autoclaved stock)
  • 0.4% carbon source (From 40% glucose filtered stock)
  • 0.25ug/ml Biotin (From filtered 1000X, 0.25mg/ml stock) - EcNR2-specific
  • 0.00005% Vitamin B1 (thiamine) (From 10,000X, 0.5% filtered stock) - MG1655-specific (see Jensen, J. Bacteriol., 1993)

Bring to volume with sterile water

5X M9 salts:
  • 64g Na2HPO4*7H2O
  • 15g KH2PO4
  • 2.5g NaCl
  • 5g NH4Cl