Team:OUC-China

From 2013.igem.org

(Difference between revisions)
 
(46 intermediate revisions not shown)
Line 1: Line 1:
<html lang="en">
<html lang="en">
<head>
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta name="viewport" content="width=device-width, initial-scale=1.0">
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
<link href="http://netdna.bootstrapcdn.com/bootstrap/3.0.0/css/bootstrap.min.css" rel="stylesheet" media="screen">
 +
<script src="http://code.jquery.com/jquery.min.js"></script>
<style>
<style>
 +
/*the igem Global setting*/
 +
* {
 +
  margin: 0;
 +
  padding: 0;
 +
  -webkit-font-smoothing: antialiased;
 +
  -webkit-box-sizing: border-box;
 +
  -moz-box-sizing: border-box;
 +
  box-sizing: border-box;
 +
}
 +
body{
 +
  color: #34495e;
 +
}
 +
#bodyContent h1, #bodyContent h2{
 +
  margin-bottom:0;
 +
}
 +
h1, h2, h3, h4, h5, h6{
 +
  padding-top: 0;
 +
  padding-bottom: 0;
 +
  color: #34495e;
 +
}
 +
#globalWrapper {
 +
  font-size: 100%;
 +
}
#top-section{
#top-section{
   height:0px;
   height:0px;
Line 25: Line 50:
}
}
#content h1.firstHeading {display: none;}
#content h1.firstHeading {display: none;}
-
    /* Carousel base class */
+
#content
-
    .carousel {
+
{
-
      margin-bottom: 60px;
+
  border:none;
-
    }
+
  height: 100%;
-
 
+
  margin:0;
-
    .carousel .container {
+
  padding:0;
-
      position: relative;
+
  width:100%;
-
      z-index: 9;
+
  position: absolute;
-
    }
+
  background-color: #FFFFFF;
-
 
+
  color: #34495e;
-
    .carousel-control {
+
}
-
      height: 80px;
+
a:visited{
-
      margin-top: 0;
+
  color: #fff;
-
      font-size: 120px;
+
}
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
/*the nav settings
-
      background-color: transparent;
+
-
      border: 0;
+
-
      z-index: 10;
+
-
    }
+
-
 
+
-
    .carousel .item {
+
-
      height: 400px;
+
-
    }
+
-
    .carousel img {
+
-
      position: absolute;
+
-
      top: 0;
+
-
      left: 0;
+
-
      min-width: 100%;
+
-
      height: 400px;
+
-
    }
+
-
 
+
-
    .carousel-caption {
+
-
      background-color: transparent;
+
-
      position: static;
+
-
      max-width: 550px;
+
-
      padding: 0 20px;
+
-
      margin-top: 200px;
+
-
    }
+
-
    .carousel-caption h1,
+
-
    .carousel-caption .lead {
+
-
      margin: 0;
+
-
      line-height: 1.25;
+
-
      color: #fff;
+
-
      text-shadow: 0 1px 1px rgba(0,0,0,.4);
+
-
    }
+
-
    .carousel-caption .btn {
+
-
      margin-top: 10px;
+
-
    }
+
-
    /* MARKETING CONTENT
+
-
    -------------------------------------------------- */
+
-
 
+
-
    /* Center align the text within the three columns below the carousel */
+
-
    .marketing .span6 {
+
-
      text-align: center;
+
-
    }
+
-
    .marketing h2 {
+
-
      font-weight: normal;
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
    .marketing .span6 p {
+
-
      margin-left: 200px;
+
-
      margin-right: 60px;
+
-
    }
+
-
    /* Featurettes
+
-
    ------------------------- */
+
-
 
+
-
    .featurette-divider {
+
-
      margin: 80px 0; /* Space out the Bootstrap <hr> more */
+
-
    }
+
-
    .featurette {
+
-
      padding-top: 120px; /* Vertically center images part 1: add padding above and below text. */
+
-
      overflow: hidden; /* Vertically center images part 2: clear their floats. */
+
-
    }
+
-
    .featurette-image {
+
-
      margin-top: -120px; /* Vertically center images part 3: negative margin up the image the same amount of the padding to center it. */
+
-
    }
+
-
 
+
-
    /* Give some space on the sides of the floated elements so text doesn't run right into it. */
+
-
    .featurette-image.pull-left {
+
-
      margin-right: 40px;
+
-
    }
+
-
    .featurette-image.pull-right {
+
-
      margin-left: 40px;
+
-
    }
+
-
 
+
-
    /* Thin out the marketing headings */
+
-
    .featurette-heading {
+
-
      font-size: 50px;
+
-
      font-weight: 300;
+
-
      line-height: 1;
+
-
      letter-spacing: -1px;
+
-
    }
+
-
 
+
-
    /* RESPONSIVE CSS
+
-
    -------------------------------------------------- */
+
-
 
+
-
    @media (max-width: 979px) {
+
-
 
+
-
      .container.navbar-wrapper {
+
-
        margin-bottom: 0;
+
-
        width: auto;
+
-
      }
+
-
      .navbar-inner {
+
-
        border-radius: 0;
+
-
        margin: -20px 0;
+
-
      }
+
-
 
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        width: auto;
+
-
        height: 400px;
+
-
      }
+
-
 
+
-
      .featurette {
+
-
        height: auto;
+
-
        padding: 0;
+
-
      }
+
-
      .featurette-image.pull-left,
+
-
      .featurette-image.pull-right {
+
-
        display: block;
+
-
        float: none;
+
-
        max-width: 40%;
+
-
        margin: 0 auto 20px;
+
-
      }
+
-
    }
+
-
 
+
-
 
+
-
    @media (max-width: 767px) {
+
-
 
+
-
      .navbar-inner {
+
-
        margin: -20px;
+
-
      }
+
-
 
+
-
      .carousel {
+
-
        margin-left: -20px;
+
-
        margin-right: -20px;
+
-
      }
+
-
      .carousel .container {
+
-
 
+
-
      }
+
-
      .carousel .item {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel img {
+
-
        height: 400px;
+
-
      }
+
-
      .carousel-caption {
+
-
        width: 65%;
+
-
        padding: 0 70px;
+
-
        margin-top: 100px;
+
-
      }
+
-
      .carousel-caption h1 {
+
-
        font-size: 30px;
+
-
      }
+
-
      .carousel-caption .lead,
+
-
      .carousel-caption .btn {
+
-
        font-size: 18px;
+
-
      }
+
-
 
+
-
      .marketing .span4 + .span4 {
+
-
        margin-top: 40px;
+
-
      }
+
-
 
+
-
      .featurette-heading {
+
-
        font-size: 30px;
+
-
      }
+
-
      .featurette .lead {
+
-
        font-size: 18px;
+
-
        line-height: 1.5;
+
-
      }
+
-
 
+
-
    }
+
-
+
-
/*the nav settings
+
----------------------------------------------- */
----------------------------------------------- */
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
.navbar-inverse .navbar-nav>.open>a, .navbar-inverse .navbar-nav>.open>a:hover, .navbar-inverse .navbar-nav>.open>a:focus{
-
color: #1abc9c;
+
  color: #1abc9c;
-
background-color: transparent;
+
  background-color: transparent;
}
}
.navbar-inverse .navbar-nav>li>a{
.navbar-inverse .navbar-nav>li>a{
-
color: #fff;
+
  color: #fff;
}
}
.navbar-inverse .navbar-nav>li>a:hover{
.navbar-inverse .navbar-nav>li>a:hover{
-
color: #1abc9c;
+
  color: #1abc9c;
-
background-color:transparent;
+
  background-color:transparent;
}
}
.navbar-inverse .navbar-brand{
.navbar-inverse .navbar-brand{
Line 223: Line 86:
}
}
.navbar-inverse .navbar-brand p{
.navbar-inverse .navbar-brand p{
-
   padding:15px 30px;
+
   padding:12px 30px;
   margin:0;
   margin:0;
 +
}
 +
.navbar-inverse .navbar-brand:hover{
 +
  color: #1abc9c;
}
}
.navbar-inverse{
.navbar-inverse{
-
border-color: transparent;
+
  border-color: transparent;
-
background: #34495e;
+
  background: #34495e;
-
font-weight: bold;
+
  font-weight: bold;
-
filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
+
  filter: progid:DXImageTransform.Microsoft.gradient(enabled = false);
}
}
.dropdown-menu{
.dropdown-menu{
-
background-color:#34495e;
+
  background-color:#34495e;
-
border:none;
+
  border:none;
-
-webkit-box-shadow:none;
+
  -webkit-box-shadow:none;
-
box-shadow: none;
+
  box-shadow: none;
}
}
.dropdown-menu>li>a{
.dropdown-menu>li>a{
-
color: #fff;
+
  color: #fff;
-
font-weight: bold;
+
  font-weight: bold;
}
}
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
.dropdown-menu>li>a:hover, .dropdown-menu>li>a:focus{
-
color:#1abc9c;
+
  color:#1abc9c;
-
background-color: transparent;
+
  background-color: transparent;
}
}
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.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
.dropdown-submenu:hover>a:after{border-left-color:#ffffff;}
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
.dropdown-submenu.pull-left{float:none;}.dropdown-submenu.pull-left>.dropdown-menu{left:-100%;margin-left:10px;-webkit-border-radius:6px 0 6px 6px;-moz-border-radius:6px 0 6px 6px;border-radius:6px 0 6px 6px;}
-
</style>
 
-
<meta name="viewport" content="width=device-width, initial-scale=1.0">
+
/*the unslider css*/
-
<meta name="description" content="">
+
.banner {
-
  <meta name="author" content="">
+
  position: relative;
-
<title>index</title>
+
  width: 100%;
 +
  overflow: auto;
 +
  font-size: 18px;
 +
  line-height: 24px;
 +
  text-align: center;
 +
  color: #fff;
 +
  background: ;
 +
  box-shadow: 0 1px 2px rgba(0,0,0,.25);
 +
}
 +
  .banner ul {
 +
    list-style: none;
 +
    width: 300%;
 +
    margin: 0;
 +
  }
 +
  .banner ul li {
 +
    display: block;
 +
    float: left;
 +
    width: 33%;
 +
    min-height: 350px;
 +
 
 +
    -o-background-size: 100% 100%;
 +
    -ms-background-size: 100% 100%;
 +
    -moz-background-size: 100% 100%;
 +
    -webkit-background-size: 100% 100%;
 +
    background-size: 100% 100%;
 +
 
 +
    box-shadow: inset 0 -3px 6px rgba(0,0,0,.1);
 +
  }
 +
 
 +
  .banner .inner {
 +
 
 +
 
 +
    padding: 160px 0 110px;
 +
  }
 +
 
 +
  .banner h1, .banner h2 {
 +
    font-size: 40px;
 +
    line-height: 52px;
 +
    border-bottom:#fff;
 +
    color: #fff;
 +
  }
 +
 
 +
  .banner .btn {
 +
    display: inline-block;
 +
    margin: 25px 0 0;
 +
    padding: 9px 22px 7px;
 +
    clear: both;
 +
 
 +
    color: #fff;
 +
    font-size: 12px;
 +
    font-weight: bold;
 +
    text-transform: uppercase;
 +
    text-decoration: none;
 +
 
 +
    border: 2px solid rgba(255,255,255,.4);
 +
    border-radius: 5px;
 +
  }
 +
    .banner .btn:hover {
 +
      background: rgba(255,255,255,.05);
 +
    }
 +
    .banner .btn:active {
 +
      -webkit-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -moz-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -ms-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      -o-filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
      filter: drop-shadow(0 -1px 2px rgba(0,0,0,.5));
 +
    }
 +
 
 +
  .banner .btn, .banner .dot {
 +
    -webkit-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -moz-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -ms-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    -o-filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
    filter: drop-shadow(0 1px 2px rgba(0,0,0,.3));
 +
  }
 +
 
 +
  .banner .dots {
 +
    position: absolute;
 +
    left: 0;
 +
    right: 0;
 +
    bottom: 20px;
 +
    margin: 0;
 +
  }
 +
    .banner .dots li {
 +
      display: inline-block;
 +
      width: 10px;
 +
      height: 10px;
 +
      margin: 0 4px;
 +
 
 +
      text-indent: -999em;
 +
 
 +
      border: 2px solid #fff;
 +
      border-radius: 6px;
 +
 
 +
      cursor: pointer;
 +
      opacity: .4;
 +
 
 +
      -webkit-transition: background .5s, opacity .5s;
 +
      -moz-transition: background .5s, opacity .5s;
 +
      transition: background .5s, opacity .5s;
 +
    }
 +
      .banner .dots li.active {
 +
        background: #fff;
 +
        opacity: 1;
 +
      }
 +
 
 +
  .banner .arrows {
 +
    position: absolute;
 +
    bottom: 20px;
 +
    right: 20px;
 +
    color: #fff;
 +
  }
 +
    .banner .arrow {
 +
      display: inline;
 +
      padding-left: 10px;
 +
      cursor: pointer;
 +
    }
 +
 
 +
/*features*/
 +
.features {
 +
  overflow: hidden;
 +
  padding: 50px 0;
 +
}
 +
  .features li {
 +
    position: relative;
 +
    padding: 0 25px 0 40px;
 +
    list-style: none;
 +
 
 +
    width: 25%;
 +
    float: left;
 +
  }
 +
    .features li:before {
 +
      content: '';
 +
      position: absolute;
 +
      left: 0;
 +
      top: 0;
 +
 
 +
      display: block;
 +
      width: 24px;
 +
      height: 22px;
 +
 
 +
      background: url('img/icons.png');
 +
    }
 +
      .features li.browser:before { background-position: 0 -44px; }
 +
      .features li.height:before { background-position: 0 -22px; }
 +
      .features li.responsive:before { background-position: 0 -66px; }
 +
  .features b {
 +
    display: block;
 +
    padding-bottom: 6px;
 +
  }
 +
  .wrap {
 +
  margin: 0 auto;
 +
  max-width: 1170px;
 +
}
 +
/*footer*/
 +
footer{
 +
  margin-top: 100px;
 +
  background-color: #eceff1;
 +
  color: #bdc1c5;
 +
  font-size: 17px;
 +
  padding: 0;
 +
}
 +
.footer-banner{
 +
  background-color: #34495e;
 +
  color: #58F8D9;
 +
  margin-left: 42px;
 +
  min-height: 286px;
 +
  padding: 0 30px 30px;
 +
}
 +
.footer-banner ul{
 +
  list-style-type: none;
 +
}
 +
.footer-banner ul li{
 +
  border-bottom:1px solid rgba(57, 107, 97, 0.53);
 +
  line-height: 19px;
 +
  padding: 6px 0;
 +
}
 +
.footer-banner a{
 +
  color: #58F8D9;
 +
}
 +
.footer-banner a:hover{
 +
  text-decoration: none;
 +
}
 +
.footer-title{
 +
  margin: 0 0 22px;
 +
  padding-top: 21px;
 +
  color: #1abc9c;
 +
  font-weight: bold;
 +
}
 +
.footerleft a{
 +
  color: #bdc1c5;
 +
}
 +
.footerleft a:visited {
 +
  color: #bdc1c5;
 +
}
 +
</style>
</head>
</head>
-
<body >
+
<body>
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
<div class="navbar navbar-inverse navbar-fixed-top" role="navigation">
-
<div class="navbar-header">
+
  <div class="navbar-header">
-
<button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
+
    <button type="button" class="navbar-toggle" data-toggle="collapse" data-target=".navbar-ex1-collapse">
-
<span class="sr-only">The navigation</span>
+
      <span class="sr-only">The navigation</span>
-
      <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
      <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
      <span class="icon-bar"></span>
+
          <span class="icon-bar"></span>
-
      </button>
+
        </button>
     <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
     <a class="navbar-brand" href="https://igem.org/Team_Wikis?year=2013">
       <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
       <img src="https://static.igem.org/mediawiki/2013/7/7d/Ouc-igem1.png">
Line 278: Line 338:
   </div>
   </div>
     <div class="collapse navbar-collapse navbar-ex1-collapse">
     <div class="collapse navbar-collapse navbar-ex1-collapse">
-
    <ul class="nav navbar-nav pull-right">
+
      <ul class="nav navbar-nav pull-right">
-
<li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
+
      <li><a href="https://2013.igem.org/Team:OUC-China">Home</a></li>
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
             <li class="dropdown" role="menu" aria-labelledby="dropdownMenu">
-
            <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
+
              <a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<b class="caret"></b></a>
-
            <ul class="dropdown-menu">
+
              <ul class="dropdown-menu">
-
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             <h1>WE DID IT !</h1>
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            <p>Constructing an Artificial Organelle（Intracellular Compartment） in <i>E.coli</i></p>
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             <h1>Compression of Metabolic Pathways</h1>
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            <p>Constructing an artificial cluster to build the compartment by operating fewer genes. </p>
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            <h1>Microfluidic Chip Magnetism Detection</h1>
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             <h1>Factors Considered in RNA guardian</h1>
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      <h3><b>Features Of Our Project</b></h3><hr>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Artificial Organelle(Intracellular Compartment)</b>
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        <h1>Abstract</h1>
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          <p>We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.</p>
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        <p class="lead">Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
+
          <a class="btn btn-info" href="https://2013.igem.org/Team:OUC-China/Results">Learn more</a>
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How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.</p>
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          <p>We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.</p>
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        <p class="lead">1.We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.<br/>2.We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.<br/>3.We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.<br/>4.We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in High oxygen partial pressure environment.
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Microfluidic</b>
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</p>
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          <p>We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, <i>Magnetospirillum Magneticum</i>.</p>
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         <p class="lead" ><font color="#0099FF">Reconstructing the Magnetosome Membrane in E. coli</font></p>
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          <b><span class="glyphicon glyphicon-ok-sign"></span> Preserving <i>mam</i>AB genes</b>
 +
          <p>We preserved <i>Magnetospirillum Magneticum</i> AMB-1 <i>mam</i>AB genes in <i>E.coli</i>, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.</p>
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        <h3><b>Introduction</b></h3><hr>
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        <p>Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production.
 +
How could a membrane be constructed in a Prokaryote? The answer may lie in this species: <i>Magnetosprillum Magneticum</i>, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in <i><i>E.coli</i></i>, creating better conditions for efficient biological production.</p>
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         <p style="text-align:center"><a class="btn btn-large btn-info" href="https://2013.igem.org/Team:OUC-China/Overview">Learn more</a></p>
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Latest revision as of 03:38, 28 September 2013

    Features Of Our Project

  • Artificial Organelle(Intracellular Compartment)

    We designed an artificial prokaryotic membranous organelle which is capable of anchoring proteins, opening up new possibilities for intracellular biochemistry reactions.

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  • RNA Guardian

    We took advantage of the 3D structure of RNA, using ribosomes as a barrier to stabilize RNA.

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  • Microfluidic

    We used Microfluidic Technology to detect the magnetism of our magnetic bacteria, Magnetospirillum Magneticum.

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  • Preserving mamAB genes

    We preserved Magnetospirillum Magneticum AMB-1 mamAB genes in E.coli, prevented the genes lose when AMB-1 strain was cultured in high oxygen partial pressure environment.

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Introduction

Putting biological resources into production has now become a hot topic since the development of technology and the draining of natural resources. For example, research about biofuel and biochemistry is now flourishing. But biological products have drawbacks of being inefficient and not broad-spectrum. Inspired by eukaryotic membranous organelles, we aim to construct a prokaryotic membranous organelle to realize division of work inside the cell and improve the efficiency of production. How could a membrane be constructed in a Prokaryote? The answer may lie in this species: Magnetosprillum Magneticum, which can form a natural intracellular membrane. But this bacteria is slow-growing and requires demanding culture conditions, so the purpose of our project is to reconstruct the magnetosome membrane in E.coli, creating better conditions for efficient biological production.

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