Team:OUC-China/Lab note

From 2013.igem.org

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            <h1>Week2</h1>
 
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  <h4>June 30th</h4>
 
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      <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Wengjun Wang
 
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      <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
 
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1. PCR: Get 16srDNA sequence from AMB-1 bacteria genome to detect the strain.<br/>
 
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2. Agarose electrophoresis: detect the PCR product.<br/>
 
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3. Transform: transform the ligastion product into Top 10 to prepare for sequence.
 
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            <h1>Week3</h1>
 
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  <h4>July 13th ~ 15th</h4>
 
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    <div class="span9"> <p style="font-weight:normal;"><font size="3px">Person: Wenjun Wang</font></p>
 
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   <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
 
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    1. Get the AMB-1 bacteria strain from Nanjing University.<br/>2. Keep the strain in 30℃.</font></p></div>
 
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      <h4>June 30th</h4>
 
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      <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Kaili Qin
 
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      <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
 
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  1. Equip the medium: Equip the medium of Magnetospirillum Magneticum AMB-1.<br/>
 
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      <h4>July 20th</h4>
 
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  <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Wenjun Wang
 
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      <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from PCR product to finish the Over-Lap PCR. <br/>
 
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    2. Agarose electrophoresis: detect the plasmid PCR product.<br/>
 
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3. Transform: transform the ligastion product into Top 10 to prepare for sequence.<br/>
 
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4. PCR: Get mamK sequence from PCR product to finish the Over-Lap PCR. <br/>
 
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5. Agarose electrophoresis: detect the PCR product.<br/>
 
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6. Product purification: purify the PCR product.<br/>
 
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7. PCR: Get mamAB sequence from AMB-1 bacteria genome.
 
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    <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Qianyun Lu
 
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      <p style="font-weight:normal;"><font size="3px">1. Culture the E.coli cells: Psb1c3.<br/>
 
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Revision as of 08:08, 26 September 2013

 










 
 
Lab-note






June 26th

Person: Wenjun Wang

 

Experiment:
1. Get the AMB-1 bacteria strain from Nanjing University.
2. Keep the strain in 30℃.