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| - | <html lang="en">
| + | <html lang="en"> |
| | <head> | | <head> |
| | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> | | <meta http-equiv="X-UA-Compatible" content="IE=edge,chrome=1"> |
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| | <meta name="description" content=""> | | <meta name="description" content=""> |
| | <meta name="author" content=""> | | <meta name="author" content=""> |
| - | <title>Lab-note</title> | + | <title>Human Practice</title> |
| | </head> | | </head> |
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| | <div class="span3 bs-docs-sidebar"> | | <div class="span3 bs-docs-sidebar"> |
| | <ul class="nav nav-list bs-docs-sidenav"> | | <ul class="nav nav-list bs-docs-sidenav"> |
| - | <li><a href="#Week1"><i class="icon-chevron-right"></i>Week1</a></li> | + | <li><a href="#Overview"><i class="icon-chevron-right"></i>Overview</a></li> |
| - | <li><a href="#Week2"><i class="icon-chevron-right"></i>Week2</a></li> | + | <li><a href="#Timeline"><i class="icon-chevron-right"></i>Timeline</a></li> |
| - | <li><a href="#Week3"><i class="icon-chevron-right"></i>Week3</a></li> | + | <li><a href="#Scientific Research Camp"><i class="icon-chevron-right"></i>Scientific Research Camp</a></li> |
| - | <li><a href="#Week4"><i class="icon-chevron-right"></i>Week4</a></li> | + | <li><a href="#Brainstorming Winter camp"><i class="icon-chevron-right"></i>Brainstorming Winter camp</a></li> |
| - | <li><a href="#Week5"><i class="icon-chevron-right"></i>Week5</a></li> | + | <li><a href="#Meeting with Dr.Wu"><i class="icon-chevron-right"></i>Meeting with Dr.Wu Longfei in CAS</a></li> |
| - | <li><a href="#Week6"><i class="icon-chevron-right"></i>Week6</a></li> | + | <li><a href="#Visit from SCAU"><i class="icon-chevron-right"></i>Visit from SCAU</a></li> |
| - | <li><a href="#Week7"><i class="icon-chevron-right"></i>Week7</a></li> | + | <li><a href="#Visit to Beijing and Tianjin"><i class="icon-chevron-right"></i>Visit to Beijing and Tianjin</a></li> |
| - | <li><a href="#Week8"><i class="icon-chevron-right"></i>Week8</a></li> | + | <li><a href="#Tour of CAS"><i class="icon-chevron-right"></i>Tour of CAS</a></li> |
| - | <li><a href="#Week9"><i class="icon-chevron-right"></i>Week9</a></li> | + | <li><a href="#Summer Camp with High School"><i class="icon-chevron-right"></i>Hands-on Experience Summer Camp with Qingdao No.2 High School</a></li> |
| - | <li><a href="#Week10"><i class="icon-chevron-right"></i>Week10</a></li> | + | <li><a href="#Summer Camp of Life Science and Technology"><i class="icon-chevron-right"></i>Summer Camp of Life Science and Technology</a></li> |
| - | <li><a href="#Week11"><i class="icon-chevron-right"></i>Week11</a></li> | + | <li><a href="#Model iGEM in Beijing"><i class="icon-chevron-right"></i>Model iGEM in Beijing</a></li> |
| - | <li><a href="#Week12"><i class="icon-chevron-right"></i>Week12</a></li> | + | <li><a href="#Seminar with Mr. Tong Zhe, Founder of One-man University"></i>Seminar with Mr. Tong Zhe, Founder of One-man University</a></li> |
| - | <li><a href="#Week13"><i class="icon-chevron-right"></i>Week13</a></li> | + | <li><a href="#Communicate with Tsinghua University"><i class="icon-chevron-right"></i>Communicate with Tsinghua University</a></li> |
| | </ul> | | </ul> |
| | </div> | | </div> |
| Line 228: |
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| | <div class="span9"> | | <div class="span9"> |
| | | | |
| - | <section id="Week1"> | + | <section id="Overview"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week1</h1> | + | <h1>Overview</h1> |
| | </div> | | </div> |
| - | <h4>June 26th</h4> | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| Line 238: |
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| | <div class="row"> | | <div class="row"> |
| | | | |
| - | <div class=" span6"><p style="font-weight:normal;"><font size="3px"> Person: Wenjun Wang</font></p> | + | <div class=" span9"><p style="font-weight:normal;"><font size="3px"> Humans' reform on nature has always been one part of its construction. Human practice is not only a question of making synthetic biology known to the world; it’s a human right for every person to know what is really going on. As iGEMers, we have the duty to make iGEM known and eliminate misunderstandings about the subject. As the only iGEM team in Shandong Province, OUC- China always puts great emphasis on making iGEM known to more people. </ font></p>< br/> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:</font><br/>
| + | <img src="https://static.igem.org/mediawiki/ igem.org/ a/ a9/Ouc- 1.jpg" height=" 500" width=" 600" /> |
| - | 1. Get the AMB-1 bacteria strain from Nanjing University.<br/>2. Keep the strain in 30℃.</p></div>
| + | |
| - | <div class="span3"> <img src="https://static.igem.org/mediawiki/ 2013/ e/ ef/Ouc- week1.jpg" height=" 200" width=" 100" / ></div> | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| - | | + | <section id="Timeline"> |
| - | | + | |
| - | <section id="Week2"> | + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week2</h1> | + | <h1>Timeline</h1> |
| | </div> | | </div> |
| - | <h4>June 30th</h4> | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
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| | <div class="row"> | | <div class="row"> |
| | | | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Wengjun Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"><br/> |
| - | </font></p>
| + | December 2012 Scientific Research Camp<br/> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
| + | February 2013 Brainstorming Winter Camp<br/> |
| - | 1. PCR: Get 16srDNA sequence from AMB-1 bacteria genome to detect the strain.<br/>
| + | March 2013 Meeting with Dr. Wu Longfei in CAS<br/> |
| - | 2. Agarose electrophoresis: detect the PCR product.<br/>
| + | Early April 2013 Visit from SCAU<br/> |
| - | 3. Transform: transform the ligastion product into Top 10 to prepare for sequence.
| + | Late April 2013 Visit to Beijing and Tianjin<br/> |
| - | </font></p> | + | May 2013 Tour of CAS<br/> |
| | + | July 2013 2nd Hands-on experience summer camp with Qingdao No.2 High School<br/> |
| | + | August 2013 3rd summer camp of Life science and technology<br/> |
| | + | September 7, 2013 Model iGEM in Beijing<br/> |
| | + | September 16, 2013 Seminar with Mr. Tong Zhe, Founder of One-man University<br/> |
| | + | </font></p><br/> |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| - |
| + | <section id="Scientific Research Camp"> |
| - |
| + | |
| - | <section id="Week3">
| + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week3</h1> | + | <h1>Scientific Research Camp</h1> |
| | </div> | | </div> |
| - | <h4>July 13th ~ 15th</h4>
| |
| | | | |
| | <div class="row"> | | <div class="row"> |
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| | <div class="row"> | | <div class="row"> |
| | | | |
| - | <div class="span9"> <p style="font-weight:normal;"><font size="3px"> Person: Wenjun Wang</font></p> | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> After last year’s OUC- China team came back from Boston, they organized a Camp aimed at potential 2013 iGEMers, with 12 iGEM direction members and 6 non-iGEM direction members. The camp members were divided into groups of three and assigned tasks every week. At the same time, the 2012 OUC- China iGEMers delivered lectures, resources, lab courses, sharing everything that they know about iGEM and scientific research. We tried out a new lab management system, which not only made a positive impact on our lab, but also on other neighbouring labs. The Scientific research camp told us about all the basics of iGEM and synthetic biology.</font></p>< br/> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
| + | <img src="https://static.igem.org/mediawiki/igem.org/e/e0/Ouc-2.jpg" height="500" width="600" /> |
| - | 1. Get the AMB-1 bacteria strain from Nanjing University.<br/>2. Keep the strain in 30℃.</font></p></div>
| + | </div> |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/0/04/Ouc-week2.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/ 2013/f/f2/Ouc-week3-2.jpg" height="200" width="100" /></div> | + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/4/4e/Ouc-week3-3.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/6/6e/Ouc-week3-4.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/8/81/Ouc-week3-5.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/f/ff/Ouc-week3-6.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/2/23/Ouc-week3-7.jpg" height="200" width="100" /></div>
| + | |
| - | <div class="span2"> <img src="https://static.igem.org/mediawiki/2013/7/79/Ouc-week3-8.jpg" height="200" width="100" /></div> | + | |
| | </div> | | </div> |
| | </div> | | </div> |
| - |
| |
| | </div><br /><br /></br> | | </div><br /><br /></br> |
| | </section> | | </section> |
| - | | + | <section id="Brainstorming Winter camp"> |
| - | </section>
| + | |
| - | <section id="Week4">
| + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week4</h1> | + | <h1>Brainstorming Winter camp</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>June 30th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Kaili Qin | + | <div class="span9"><p style="font-weight:normal;"><font size="3px">The 2013 iGEMers arrived on campus on February 22, ten days before the start of the spring semester. We were there because we needed time to get together in the winter holidays and discuss what we learned during the holidays. <br/> |
| - | </font></p>
| + | In those ten days, we got up early every morning and went for a run around the field, then signing our team song, Life in Full Bloom. We read papers and news, discussed ideas and studied past projects. On the last day, we invited professors to give advice on the ideas that we showed them. </font></p><br> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>
| + | <div class=" span4">< img src=" https://static.igem.org/mediawiki/2013/7/7c/Ouc- 3.jpg" height=" 300" width="300" /></ div> |
| - | 1. Equip the medium: Equip the medium of Magnetospirillum Magneticum AMB-1.<br/>
| + | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/e/ee/Ouc-4.jpg" height="300" width="300" /></div> |
| - | </font></p></div>
| + | |
| - | <h4>July 20th</h4>
| + | |
| - | <div class=" span9">< p style=" font- weight:normal;" ><font size=" 3px" >Person: Wenjun Wang | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from PCR product to finish the Over-Lap PCR. <br/>
| + | |
| - | 2. Agarose electrophoresis: detect the plasmid PCR product.<br/>
| + | |
| - | 3. Transform: transform the ligastion product into Top 10 to prepare for sequence.<br/>
| + | |
| - | 4. PCR: Get mamK sequence from PCR product to finish the Over-Lap PCR. <br/>
| + | |
| - | 5. Agarose electrophoresis: detect the PCR product.<br/>
| + | |
| - | 6. Product purification: purify the PCR product.<br/>
| + | |
| - | 7. PCR: Get mamAB sequence from AMB-1 bacteria genome.
| + | |
| - | </font></p></div>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">1. Culture the E.coli cells: Psb1c3.<br/>
| + | |
| - | </font></p></div>
| + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| - | | + | <section id="Meeting with Dr.Wu"> |
| - | <section id="Week5"> | + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week5</h1> | + | <h1>Meeting with Dr.Wu</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>July 21st</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Wenjun Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> We met Dr.Wu Longfei by chance when he came to OUC to give a lecture. We did not know about him before. How excited we were when he started talking about MMB! After the lecture, we had a long chat, resulting in agreement for further cooperation. We arranged to meet in Institute of Oceanology, Chinese Academy of Sciences. |
| - | </font></p>
| + | At Chinese Academy of Sciences, we thoroughly explained our project to Dr.Wu Longfei and Dr.Xiao Tian. They were willing to provide us with the strain that we need, <i>Magnetospirillum Magneticum</i> AMB-1.</font></p> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Agarose electrophoresis: detect the plasmid PCR product.<br/>
| + | <br/> |
| - | 2. PCR: Get mamB sequence from PCR product.<br/>
| + | <div class=" span4">< img src=" https:// static. igem. org/ mediawiki/ 2013/ 8/ 8b/ Ouc- 5. jpg" height=" 300" width=" 300" /></div> |
| - | 3. PCR: Get mamQ sequence from PCR product.<br/>
| + | <div class=" span4">< img src=" https:// static. igem. org/ mediawiki/ 2013/1/ 1e/ Ouc- 6. jpg" height=" 300" width=" 300" /></div> |
| - | 4. PCR: Get mamJ sequence from PCR product to finish the Over-Lap PCR. <br/>
| + | |
| - | 5. Agarose electrophoresis: detect the plasmid PCR product.<br/>
| + | |
| - | 6. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person: Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination: E.coli with part K1059003-B0035-GFPlva-K1059004-B0015 pSB1AK3 as backbone; E.coli with RFP, pSB1C3 as backbone.
| + | |
| - | </font></p></div>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of PSB1C3 for 5 tubes.<br/>2. Electrophoresis: gel is good.<br/>3. Equip the medium: Equip the medium of LB (solid).
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR production purification: 16SrRNA from AMB-1.<br/>2. SDS page gel electrophoresis: the purified PCR production.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR genome for mamI gene & PCR purification.<br/>2. PCR genome for mamB gene.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 22nd</h4>
| + | |
| - |
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: Plasmid pSB1AK3 with K1059003-B0035-GFPlva-K1059004-B0015, and pSB1C3 with RFP.<br/>2. Enzyme digestion.<br/>3. Gel extraction.<br/>4. Recombination: Get part K1059003-B0035-GFPlva-K1059004-B0015, pSb1C3 as backbone.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1.Enzyme digestion: enzyme digestion of PSB1C3 for 25ul system.<br/>2.Gel recovery: gel recovery of the product of enzyme digestion.<br/>3.Bacterination: bacterinate PSB1C3-RFP, RNA1 into tubes of 10ml.<br/>4.Pick bacteria: pick bacteria of PSB1C3-RFP for conservation.<br/>5.Genomic DNA extraction: extracting genomic DNA of E.coli.<br/>6. Electrophoresis: gel is bad.<br/>7. Bacterination: bacterinate PSB1C3-RFP, RNA1 into tubes of 10ml for 4 tubes.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: ligate the purified PCR production with T vector.<br/>2. Transformation of Top10.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 23rd</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Extraction of genomic DNA from E.coli.<br/>2. Pick the bacteria and culture the E.coli cells for four hours.<br/>3. PCR: the cultured E.coli cells.<br/>4. SDS page gel electrophoresis: PCR production.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of PSB1C3 -RFP for 5 tubes.<br/>
| + | |
| - | 2. Enzyme digestion: enzyme digestion of PSB1C3 for 50ul system of 3 tubes, use EcoR1, Pst1.3. Electrophoresis: gel is good.<br/>4. Genomic DNA extraction: extracting genomic DNA of E.coli.<br/>5. Electrophoresis: gel is bad.<br/>6. Gel recovery: gel recovery of PSB1C3.<br/>7. Electrophoresis: gel is good for 50ng/ul each.<br/>8. Bacterination: bacterination of E.coli for 6 tubes.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation: Plate cultivation for 14h.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 24th</h4>
| + | |
| - |
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Extraction of genomic DNA from E.coli.<br/>2. PCR: the cultured E.coli cells.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Genomic DNA extraction: extracting genomic DNA of E.coli.<br/>2. Electrophoresis: gel is bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Agarose electrophoresis: detect the plasmid PCR product.<br/>2. PCR: Get mamI sequence from PCR product.<br/>3. PCR: Get mamL sequence from PCR product.<br/>4. PCR: Get mamK sequence from PCR product to finish the Over-Lap PCR. <br/>5. Agarose electrophoresis: detect the plasmid PCR product.<br/>6. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria and PCR examining, the aim fragment is 1409bp.<br/>2. Sequencing.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 25th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Try to get mamAB gene cluster sequence from AMB-1 bacterial strain.<br/>2. Get the genome of AMB-1 with TaKaRa MINI Best bacteria genome DNA Extraction Ver2.0.<br/>3. PCR: Try to get mamAB gene cluster sequence from AMB-1 genome.<br/>4. Agarose electrophoresis: detect the plasmid PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. d1 to d6 as primer PCR, get part K1059005.<br/>2. Purification.<br/>3. Recombine PCR product to pMD-19 T vector.<br/>4. Transformation.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1.Equip the medium：Equip the medium of AMB-1.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis: PCR production and the genomic DNA.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation of E.coli (top10): <br/>
| + | |
| - | Part: mamK+T vector <br/>
| + | |
| - | Part: mamI+T vector<br/>
| + | |
| - | Part: mamL+T vector<br/>
| + | |
| - | Part: mamQ+T vector<br/>
| + | |
| - | Part: mamB+T vector (failure)
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 26th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Blue-white selection.<br/>2. PCR examining, the aim fragment is 388bp.<br/>3. Sequencing.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination: bacterinate PSB1C3-RFP into tubes of 10ml.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 27th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Measuring Growth curve, E.coli with RFP, pSB1C3 as plasmid backbone.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Extraction of genomic DNA from E.coli.<br/>2. SDS page gel electrophoresis: the genomic DNA.
| + | |
| - | | + | |
| - | </font></p></div>
| + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| - | | + | <section id="Visit from SCAU"> |
| - |
| + | |
| - | | + | |
| - | | + | |
| - | <section id="Week6">
| + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week6</h1> | + | <h1>Visit from SCAU</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>July 28th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px">At the beginning of April this year, seven team members from team SCAU-China paid us a visit. It’s their first year in iGEM, so we invited last year’s iGEMers to give them lectures and answer their questions. Members of the two teams went hiking together, discussing their projects and exchanging their experiences. The visit established the basis for further cooperation. </font></p><br/> |
| - | </font></p>
| + | <img src="https://static.igem.org/mediawiki/2013/1/10/Ouc-7.jpg" height="500" width="600" /> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Measure Growth curve. | + | |
| - | </font></p></div>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px"> Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination: bacterinate PSB1C3-RFP, E.coil with single-1 protection sequence into tubes of 10ml.
| + | |
| - | </font></p></div>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Culture the E.coil cells: PSB1C3.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>July 29th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial culturing.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamB sequence from AMB-1 bacterial strain.<br/>2. PCR: Get mamJ sequence from AMB-1 bacterial strain.<br/>3. PCR: Get mamE sequence from AMB-1 bacterial strain.<br/>4. PCR: Get mamE sequence from AMB-1 bacterial strain.<br/>5. Agarose electrophoresis: detect the DNA digestion product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of PSB1C3-RFP, E.coil with single-1 protection sequence for 2 tubes each.<br/>2. Enzyme digestion: enzyme digestion of PSB1C3 and E.coil with single-1 protection sequence for 25ul system of 2 tubes each, use EcoR1, Pst1.<br/>3. Electrophoresis: gel is good.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: Psb1c3.<br/>2. Digestion: digest the plasmid with EcoRI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.
| + | |
| - |
| + | |
| - | </font></p></div>
| + | |
| - | <h4>July 30th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Lstwenmin1 to Lstwenmin4 as primer PCR, get part K1059006.<br/>2. Purification.<br/>3. Recombine PCR product to pMD-19 T vector.<br/>4. Transformation.
| + | |
| - |
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transform: transform the ligastion (single-1 protection sequence-PSB1C3) product into Top 10.<br/>2. Bacterial coating.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>July 31st</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Blue-white selection.<br/>2. PCR and electrophoresis examining, the aim fragment is 388bp and 210.<br/>3. Sequencing.<br/>4. The PCR result a fragment at 120bp, so we failed the part k1059006. We designed another two primers and be ready for more PCR.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria for PCR.<br/>2. PCR: use PCR to detect the bacteria we want (single-1 protection sequence-PSB1C3)), 25ul system.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Glycerol stocks: Psb1c3, 2 tubes.<br/>2. Miniprep: Psb1c3.<br/>3. Digestion: digest the plasmid with EcoRI and Pst-HF.<br/>4. Gel extraction of DNA.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 1st</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination.<br/>2. E.coil with part K1059005 PMD-19 T vector as backbone.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR genome for mamB, mamJ, mamE, mamC gene.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamC sequence from AMB-1 bacterial strain.<br/>2. Agarose electrophoresis: detect the mamC PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. PCR: Get the mamC sequence which is more nicety from the product of product purification.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 2nd</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>2. Enzyme digestion.<br/>3. Recombination: Get part K1059005-B0035-GFPlva-K1059004-B0015, pSB1C3 as backbone.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation of E.coil (top10): mamB+T vector.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR：Get mamB, mamE sequence from AMB-1 bacterial strain.<br/>2. Get the genome of AMB-1.<br/>3. Transform: transform the ligastion product into Top 10.
| + | |
| - | </font></p></div>
| + | |
| - | <h4>August 3rd</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation and Bacterination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: Psb1c3, 6 tubes.<br/>2. Digestion: digest the plasmid with EcoRI and Pst-HF, XbaI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. E. coli Cell Culture: Top10 (mamB+T vector) Resistance: Ampicillin Medium: LB.<br/>2. mamK PCR purification: Get mamK gene.<br/>3. PCR genome for mamB: 16x50ul PCR system. Get mamB gene.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Agarose electrophoresis: detect the PCR product.<br/>2. Get the genome of AMB-1.<br/>3. Agarose electrophoresis: detect the genome product.<br/>4. PCR: to detect the product of transform.<br/>5. PCR: Get mamC sequence from AMB-1 bacterial strain.<br/>6. PCR: Get 16srDNA sequence from AMB-1 bacterial strain to detect the strain.<br/>7. Agarose electrophoresis: detect the genome product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | | + | <section id="Visit to Beijing and Tianjin"> |
| - | <section id="Week7"> | + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week7</h1> | + | <h1>Visit to Beijing and Tianjin</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>August 4th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> We visited Tianjin and Peking iGEM teams in order to learn from their experiences. The iGEMers from Beijing and Tianjin welcomed us and showed us around their labs. We talked about our projects and had conferences about lab management and advances in synthetic biology. OUC-China is a relatively “young” team, so we learnt a lot from these “old” teams.</font></p><br/> |
| - | </font></p>
| + | <div class=" span4"><img src="https://static.igem.org/mediawiki/2013/9/ 98/Ouc-8.jpg" height=" 300" width="300" /></div> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: J23101 order vector with chloramphenicol resistance; double2 order
| + | <div class=" span4"><img src="https://static.igem.org/mediawiki/2013/7/ 7b/Ouc-9.jpg" height=" 300" width="300" /></div> |
| - | RBS-GFPLVA- single2-terminator and vector with chloramphenicol resistance.<br/>2. Transform: transform the ligastion product into Top 10.<br/>3. Bacterial coating
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get BBa_K1059006 sequence from PCR product.<br/> 2. Agarose electrophoresis: detect the PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. Ligation: recombine PCR product to pMD-19 T vector.<br/>5. Sequencing<br/>6. Transformation: transform the ligastion product into Top 10.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - |
| + | |
| - | <h4>August 5th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Blue -white selection.<br/>2. PCR and electrophoresis examining.<br/>
| + | |
| - | 3. PCR and electrophoresis examining, get the aim fragment.<br/>4. Sequencing.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Culture the E.coli cells: pSB1C3.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria for PCR.<br/>2. PCR: use pcr to detect the bacteria we want.(double2-RBS-GFPLVA-single2-terminator-PSB1C3), 25ul system.<br/>3. Electrophoresis.<br/>4. Send sequencing.<br/>5. Conservation: conservate E.coli with.double2-RBS-GFPLVA-single2-terminator-PSB1C3 plasmid.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 6th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/8/85/Ouc-week4.jpg" height="400" width="300" /> | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: pSB1C3.<br/>2. Digestion: digest the plasmid with XbaI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.<br/>5. PCR production purification: J23106+RBS, mamQ, mamL, B0015.<br/>6. SDS page gel electrophoresis: J23106+RBS, mamQ, mamL, B0015.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/9/ 99/Ouc- week5.png" height="400" width="300" /><br/>100bp, J23106+RBS, mamQ, mamL, B0015<br/>7. PCR amplification: J23106+RBS, B0015.<br/>8 . SDS page gel electrophoresis: J23106+RBS, B0015. | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from PCR product and add prefix and suffix onto the sequence.<br/>2. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. Agarose electrophoresis: detect the PCR product.<br/>4. Product purification: purify the PCR product.<br/>5. Ligation: BBa_J23101-protection order 1-RBS-GFPLVA-terminator and vector with chloramphenicol resistance.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/f/f4/Ouc-week6.jpg" height="400" width="300" />
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of pSB1C3 -RFP for 5 tubes.<br/>2. Enzyme digestion: enzyme digestion of pSB1C3 for 50ul system of 3 tubes, use Xba1, Pst1.<br/>3. Electrophoresis: gel is good.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/ c/c7/Ouc-week7.jpg" height="400" width="300" /> | + | |
| - | </font></p></div>
| + | |
| - | <h4>August 7th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transform: transform the ligastion product into Top 10.<br/>2. PCR: Get mamL sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the sequence.<br/>4. Agarose electrophoresis: detect the PCR product.<br/>5. PCR: Get J23106+B0032 sequence from PCR product and add prefix and suffix onto the sequence.<br/>6. PCR: Get B0015 sequence from PCR product and add prefix and suffix onto the sequence.<br/>7. Agarose electrophoresis: detect the PCR product.<br/>8. Product purification: purify the PCR product.<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Gel recovery: gel recovery of pSB1C3.<br/>2. Electrophoresis: gel is good for 30ng/ul each.<br/>3. PCR: use PCR to B0015 cloning, 50ul system.<br/>4. Electrophoresis: gel is good. <br/>5. PCR Purification: PCR Purification of B0015.<br/>6. Electrophoresis: gel is good for 30ng/ul each.<br/>7. Enzyme digestion: enzyme digestion of RBS-mamL for 25ul system, use Xba1, Pst1;RBS-mamL for 25ul system, use Spe1; B0015 for 25ul system, use Xba1; promoter-RBS for 25ul system, use EcoR1, Pst1.<br/>8. Ligation: RBS-mamL order pSB1C3; promoter-RBS order pSB1C3; RBS-mamL order B0015.<br/>9. Transform: transform the ligastion product into Top 10.<br/>10. Bacterial coating.<br/>11. Bacterination: bacterinate E.coli with PSB1C3-RFP into tubes of 10ml.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamL, three tubes.<br/>2. PCR production purification: mamL, three tubes; J23106+RBS, three tubes.<br/> 3. SDS page gel electrophoresis: mamL, three tubes; J23106+RBS, three tubes.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/1/1e/Ouc-week8.png" height="400" width="300" /><br/>J23106+RBS, mamL<br/>4. Digestion: digest RBS+ mamL with XbaI and Pst-HF, digest RBS+mamL with SpeI, digest B0015 with XbaI, digest J23106+RBS with EcoRI and Pst-HF.<br/>5. SDS page gel electrophoresis.<br/>6. Ligation: ligate RBS+mamL with Psb1c3, ligate J23106+RBS with pSB1C3,ligate RBS+ mamL with B0015.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 8th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep the pSB1C3, J23101, RGL-K1059005.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 9th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Enzyme digestion: BBa_J23101 with EcoR I and SpeI, BBa_ K1059002 with XbaI and PstI, BBa_K1059005 with EcoR I and PstI, pSB1C3 with EcoRI and PstI.<br/>2. Gel extraction pSB1C3 as vector.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: pSB1C3, ten tubes.<br/>2. Digestion: three tubes are digested with XbaI and Pst-HF, two tubes are digested with EcoRI and Pst-HF.<br/>3. SDS page gel electrophoresis.<br/>4. Gel extraction of DNA.<br/>5. PCR amplification: mamQ, four tubes. <br/>6. SDS page gel electrophoresis.<br/>7. PCR production purification: mamQ, two tubes.<br/>8. SDS page gel electrophoresis.<br/>9. Digestion: digest J23106+RBS with EcoRI and SpeI, digest RBS+mamQ with XbaI and Pst-HF.<br/>10. PCR amplification: RBS+mamL, four tubes.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria for PCR.<br/>2. PCR: use pcr to detect the bacteria we want(promoter-RBS-PSB1C3, RBS-mamL-PSB1C3), 25ul system.<br/>3. Enzyme digestion: enzyme digestion of PSB1C3 for 50ul system, 3 tubes, use Xba1, Pst1; pSB1C3 for 25ul system of 2 tubes, use EcoR1, Pst1.<br/>4. PCR: use pcr to cloning RBS-mamL-B0015, 50ul system.<br/>5.Electrophoresis: gel is good. <br/>6. Gel recovery: gel recovery of pSB1C3.<br/>7. Electrophoresis: gel is good for 20ng/ul each.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/d/d9/Ouc-week9.jpg" height="400" width="300" /><br/>Electrophoresis of PSB1C3 vector<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 10th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Recombination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: J23106+RBS, two tubes; B0015, two tubes. <br/>3. PCR production purification: RBS+mamL, four tubes.<br/>4. SDS page gel electrophoresis.<br/>5. Digestion: digest RBS+mamL with SpeI.<br/>6. Culture the E.coli cells: JB11.<br/>7. PCR production purification: mamI.<br/>8. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Product purification: purify the PCR product, J1.<br/>2. Plastid extraction: B0015, J2310, C+.<br/>3. Digestion: use enzyme XbaI, Pst I to get part B0015, a double terminator. <br/>4. Ligation: mamK onto pMB-19 T, prepare for sequence.<br/>5. PCR: amplification mamJ sequence from PCR product.<br/>6. Agarose electrophoresis: detect the PCR product.<br/>7. Get part for agrose, mamJ.<br/>8. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>9. Agarose electrophoresis: detect the PCR product.<br/>10. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamL1~4.<br/>2. Conservation: promoter-RBS-PSB1C3 (P4), RBS-mamL-PSB1C3 (L4).<br/>3. Ligation: RBS-mamJ, RBS-mamI, RBS-mamB order PMD-19 vector separately.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | | + | <section id="Tour of CAS"> |
| - | <section id="Week8"> | + | |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week8</h1> | + | <h1>Tour of CAS</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>August 11th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> We took a tour of the Institute of Oceanology, Chinese Academy of Sciences. We learned about many kinds of new technology and scientific advances.</font></p><br/> |
| - | </font></p>
| + | <div class=" span4">< img src=" https:// static. igem. org/ mediawiki/ 2013/ 0/ 0f/ Ouc-10. jpg" height=" 600" width=" 500" /></div> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.<br/>2. Plate cultivation for 14h. | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamQ1~10, 25ul system.<br/>2. Electrophoresis: gel is bad.<br/>3. Transform: transform the ligastion product ( RBS-mamJ-T vector;RBS-mamI-T vector, RBS-mamB-T vector, RBS-mamK-T vector) into Top 10.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep: JB11, B0015, each two tubes.<br/>2. Digestion: digest JB11 with EcoRI and SpeI, digest B0015 with XbaI and Pst-HF, digest RBS+mamL with XbaI and SpeI.<br/>3. Ligation: ligate RBS+J23106 with pSB1C3, ligate RBS+mamL and BOO15 with pSB1C3.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 12th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria and PCR examining
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transform: transform the ligastion product(RBS-mamL-B0015-PSB1C3; promoter-RBS-PSB1C3) into Top 10.<br/>2. Bacterial coating.<br/>3. Pick bacteria: pick bacteria we want(RBS-mamL-B0015-PSB1C3, promoter-RBS-mamQ-PSB1C3).
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: the cultured E.coli cells.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 13th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: the cultured E.coli cells.<br/>2. SDS page gel electrophoresis.<br/>3. Ligation: ligate the purified mamB and mamL with T vector.<br/>4. PCR amplification: mamB, mamK, mamJ.<br/>5. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Sequencing.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 14th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamJ.<br/>2. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want(RBS-mamL-B0015-PSB1C3, promoter-RBS-mamQ-PSB1C3).
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination.<br/>2. Make competent cells.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 15th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: get mamJ sequence from AMB-1 bacterial strain.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. PCR: get mamC sequence from AMB-1 bacterial strain.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep.<br/>2. Make gel.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: mamL.<br/>3. PCR production purification: RBS+mamL.<br/>4. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligastion: mamJ, mamK order PMD19-T vector.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 16th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Enzyme digestion.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamK-pMD19-T vector1~15, 25ul system.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamL.<br/>2. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 17th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamC sequence from PCR product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.<br/>4. PCR: amplification mamI sequence from PCR product.<br/>5. PCR: amplification mamJ sequence from PCR product.<br/>6. Agarose electrophoresis: detect the plasmid PCR product.<br/>7. Product purification: purify the PCR product.<br/>8. PCR: amplification mamB sequence from PCR product.<br/>9. Agarose electrophoresis: detect the plasmid PCR product.<br/>10. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. PCR amplification: mamQ, two tubes.<br/>3. PCR production purification: RBS+mamL.<br/>4. SDS page gel electrophoresis.<br/>5. PCR amplification: mamK, two tubes; mamB, two tubes.<br/>6. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: mamK-PMD19-T vector1~15, gel is bad.<br/>2. Pick bacteria: pick bacteria we want (mamK-PMD19-T vector1~10).<br/>3. PCR: use PCR to detect mamK-pMD19-T vector1~10, 25ul system.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Recombination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | <section id="Week9"> | + | <section id="Summer Camp with High School"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week9</h1> | + | <h1>Summer Camp with High School</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>August 18th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px">This year, we organized a summer camp enrolling 24 students from Qingdao No.2 High School, introducing them to the biolab and motivating their interest in Bioscience. We arranged many fun science activities for them, such as analyzing samples of marine organisms, making their own specimens, and making drinks out of artificial food additives, which left a deep impression on them about food safety. We concentrated on biosafety, hoping to raise their awareness on the subject of experimental safety.</font></p><br/> |
| - | </font></p>
| + | <div class=" span4"><img src="https://static.igem.org/mediawiki/2013/ 0/ 00/Ouc- 11. jpg" height=" 300" width="300" /></div> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.<br/>2. Plate cultivation for 14h. | + | <div class=" span4"><img src="https://static.igem.org/mediawiki/2013/ 0/ 03/Ouc- 12.jpg" height=" 300" width="300" /></div> |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px"> Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Gel recovery: gel recovery of mamE.<br/>2. Electrophoresis: gel is good for 8ng/ul each.<br/>3. Bacterination: bacterination of E.coli with PSB1C3 plasmid for 3 tubes, 10ml each.<br/>4. Bacterial coating: JQ11, QP11, KP1, BP1, BP2, LP21.<br/>5. Gel recovery: gel recovery of PSB1C3 vector.<br/>6. Electrophoresis: gel is good for 20ng/ul each.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/0/06/Ouc-week10.jpg" height="400" width="300" /><br/>Electrophoresis of PSB1C3 vector<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamI, two tubes; mamQ, two tubes.<br/>2. SDS page gel electrophoresis.<br/>3. PCR production purification: RBS+mamQ, RBS+mamI.<br/>4. SDS page gel electrophoresis.<br/>5. PCR amplification: mamI, two tubes; mamJ, two tubes.<br/>6. SDS page gel electrophoresis.<br/>7. Transformation: JQ11, QP11, BP1, BP2, LP21, KP1.<br/>8. SDS page gel electrophoresis.<br/>9. PCR amplification: mamI, two tubes; mamB, two tubes.<br/>10. SDS page gel electrophoresis: mamK; mamB.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/ 6/ 64/Ouc- week11. png" height=" 400" width="300" / ><br/>lane6 mamK1; lane7 mamK2; lane8 mamB<br/> | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Digestion: use enzyme EcoR I, Pst I to Cut PSB1C3 plasmid vector.<br/>2. Digestion: use enzyme EcoR I, Pst I to Cut promoter J23106 and RBS B0032. <br/>3. PCR: amplification mamE, mamJ sequence from PCR product.<br/>4. Agarose electrophoresis: detect the plasmid PCR product.<br/>5. Ligastion: promoter, RBS and mamQ.<br/>6. Ligastion: mamQ and PSB1C3.<br/>7. Ligastion: mamB and PSB1C3.<br/>8. Ligastion: mamL and PSB1C3.<br/>9. Ligastion: mamK and PSB1C3.<br/>10. Transform: transform the ligastion product into Top 10.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 19th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria and PCR examining.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect mamK-PSB1C3 vector1~10,25ul system.<br/>2. Electrophoresis: gel is bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: ligate the purified mamE with T vector.<br/>2. PCR production purification: RBS+mamB, two tubes.<br/>3. Pick the bacteria and culture the E.coli cells for four hours.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamE sequence from PCR product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. PCR: amplification mamI sequence from PCR product.<br/>4. Product purification: purify the PCR product.<br/>5. Agarose electrophoresis: detect the PCR product purification.<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 20th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR production purification: RBS+mamI, two tubes; RBS+mamB, one tube.<br/>3. SDS page gel electrophoresis.<br/>4. PCR amplification: mamI, four tubes.<br/>5. SDS page gel electrophoresis.<br/>6. Pick the bacteria and culture the E.coli cells for four hours.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Sequencing J23101.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to detect promoter-RBS-mamQ-PSB1C3 vector1~10, 25ul system; mamK - PSB1C31~28, 25ul system; mamE-PMD19-T vector1~8, 25ul system.<br/>2. Electrophoresis: gel is bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 21st</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. Miniprep: pSB1C3.<br/>3. Digestion: three tubes are digested with XbaI and Pst-HF, two tubes are digested with EcoRI and Pst-HF.<br/>4. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamL standard, mamB standard, mamQ standard, mamK standard.<br/>2. Electrophoresis: mamL, K, B gel is good; mamQ, gel is bad.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/ b/ b1/Ouc- week12.jpg" height=" 400" width="300" / ><br/>Electrophoresis of production of mamK,B cloning<br/> | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial activation and culturing, including control group and all experimental groups.<br/>2. Preparing for measuring the fluorescence.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 22th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamQ sequence from PCR product and add prefix and suffix onto the squence.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterialculturing.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes; mamK, four tubes.<br/>2. SDS page gel electrophoresis.<br/>3. Gel extraction of DNA.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamL, K, B.<br/>2. Enzyme digestion: enzyme digestion of mamL, K, B for 25ul system separately, use Xba1, Pst1.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 23rd</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Measuring the fluorescence.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: production of mamL, K, B enzyme digestion, gel is good.<br/>2. Ligastion: mamL, K, B order pSB1C3 vector with chloramphenicol resistance.<br/>3. Transform: transform the ligastion product into Top 10.<br/>4. Bacterial coating.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Ligation: ligate RBS+ mamB with pSB1C3, ligate RBS+mamL and BOO15 with pSB1C3, ligate RBS+ mamK with pSB1C3.<br/>2. PCR amplification: mamB, four tubes.<br/>3. Transformation: L-P, B-P, K-P.<br/>4. SDS page gel electrophoresis.<br/>5. Ligation: ligate the purified mamC and mamE with T vector.<br/>6. Transformation: C-T, E-T.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>2. PCR: Get mamL sequence from PCR product and add prefix and suffix onto the sequence.<br/>3. PCR: Get mamB sequence from PCR product and add prefix and suffix onto the sequence.<br/>4. Agarose electrophoresis: detect the plasmid PCR product.<br/>5. Product purification: purify the PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 24th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: amplification mamB+mamL, mamQ+mamI sequence from DNA ligastion product.<br/>2. Agarose electrophoresis: detect the plasmid PCR product.<br/>3. PCR: Get mamI sequence from AMB-1 bacterial strain.<br/>4. PCR: Get mamI sequence from PCR product and add prefix and suffix onto the sequence.<br/>5. Product purification: purify the PCR product.<br/>
| + | |
| - | 6. Agarose electrophoresis: detect the plasmid PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: C-T, E-T, L-P, B-P, K-P.<br/>3. SDS page gel electrophoresis: PCR production.<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamL, K, B-PSB1C3).<br/>2. PCR: use PCR to detect mamL, K, B-PSB1C3 vector1~10, 25ul.<br/>3. Electrophoresis: gel is bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Result analysis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | <section id="Week10"> | + | <section id="Summer Camp of Life Science and Technology"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week10</h1> | + | <h1>Summer Camp of Life Science and Technology</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>August 25th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> It’s our third year organizing the Science and Technology Camp, aimed at the new sophomore students. In order to ensure that the camp members have an overall understanding of Life Sciences, we gave then lectures on biochemistry, took them on a field trip to do botany sampling, taught them to do molecular experiments and showed them new realms and advances in science such as bioinformatics, microchip technology, system biology, and of course, synthetic biology.In addition, we organized a mini-Jamboree, because academic Jamboree is a relatively new concept in China, and we would like young undergraduates to know more about it. The members of the summer camp prepared their own projects during the summer holidays. During the event, they gave presentations and explained their posters, while we tried our best to create the right atmosphere for a Jamboree. The poster reception attracted many students around the campus, filling the members with satisfaction from completing their own project.</font></p><br/> |
| - | </font></p>
| + | <div class=" span4">< img src=" https:// static. igem. org/ mediawiki/ 2013/ a/ aa/ Ouc- 13.jpg" height=" 300" width=" 300" /></div> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial culturing, including control group and all experimental group.
| + | <div class=" span4">< img src=" https:// static. igem. org/ mediawiki/ 2013/ 0/ 04/ Ouc- 14. jpg" height=" 300" width=" 300" /></div> |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/> 1. Ligastion: mamC, mamE order pMD19-T vector. <br/>2. Transform: transform the ligastion product into Top 10.<br/>3. Bacterial coating.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: L-P.<br/>2. SDS page gel electrophoresis: mamC; mamE.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/3/3f/Ouc-week13.png" height="400" width="300" /><br/>lane5: mamC, lane6: mamE<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Wenjun Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: Get mamJ sequence from AMB-1 bacterial strain.<br/>2. Digestion: use enzyme XbaI I, Spel I to cut mamI, mamL, mamB, mamQ.<br/>3. Plasmid Extraction: extract the pSB1C3 plasmid which containing B0015 from E.coli.<br/>4. Agarose electrophoresis: detect the plasmid extraction product and PCR product.<br/>5. Ligastion: mamB and mamL; mamQ and mamI.<br/>6. Agarose electrophoresis: detect the DNA digestion product.<br/>7. Bacterial inoculation: inoculate the E.coli which contain pSB1C3 vector plasmid, C+.<br/>8. PCR: amplification mamB+mamL, mamQ+mamI sequence from DNA ligastion product.<br/>9. Agarose electrophoresis: detect the plasmid PCR product.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 26th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>2. Enzyme digestion and electrophoresis examining.<br/>3. Recombination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamC, E-pMD19T- vector).<br/>2. PCR: use PCR to detect mamC, E-pMD19T- vector1~10, 25ul.<br/>3. Electrophoresis: gel is bad.<br/>4. Pick bacteria: pick bacteria we want(mamC,E-PMD19T- vector ).
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours.<br/>2. PCR: Q-P, K-P.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 27th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick the bacteria and culture the E.coli cells for four hours: K-P.<br/>2. PCR: Q-P, K-P.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <h4>August 28th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes.<br/>2. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamC standard.<br/>2. PCR Purification: PCR Purification of mamL, K, B.<br/>3. Electrophoresis: gel is good.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR amplification: mamB, four tubes.<br/>2. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 29th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria.<br/>2. PCR.<br/>3. Electrophoresis examining.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Digestion: digest RBS+mamK with XbaI and Pst-HF, digest RBS+mamB with XbaI and Pst-HF.<br/>2. PCR amplification: mamE, three tubes; mamQ, three tubes, mamL, two tubes.<br/>3. SDS page gel electrophoresis.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR Purification: PCR Purification of mamE, L, Q.<br/>2. Electrophoresis: gel is good.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <h4>August 30th</h4>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria, PCR and electrophoresis examining.<br/>2. We find none of the fragment conforms to the aimed length, we explain it by the wrong proportion of part and plasmid backbone, so we prepared another experiment.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamI standard.<br/>2. Ligastion: mamL order pSB1C3 vector with chloramphenicol resistant.<br/>3. Bacterination: bacterinate JB11 (promoter-RBS-PSB1C3), into 3 tubes of 10ml each.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>2. Digestion: digest RBS+mamL with XbaI and Pst-HF, digest RBS+mamQ with XbaI and Pst-HF.<br/>3. PCR amplification: mamQ, two tubes; mamK, two tubes.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>August 31st</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. SDS page gel electrophoresis.<br/>
| + | |
| - | 2. PCR amplification: mamB, two tubes; mamK, two tubes.<br/>3. PCR production purification: RBS+mamB, two tubes.<br/>4. Digestion: digest RBS+mamB with XbaI and Pst-HF.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid extraction of E.coli with JB11 (promoter-RBS-PSB1C3) plasmid for 6 tubes.<br/>2. Enzyme digestion: enzyme digestion of JB11 (promoter-RBS-PSB1C3) for 25ul system, use Spe1, Pst1; enzyme digestion of mamQ for 25ul system, use Xba1, Pst1.enzyme digestion of mamB for 25ul system, use Xba1, Pst1.<br/>3. PCR Purification: PCR Purification of mamC.<br/>4. Electrophoresis: gel is good.<br/>5. Ligastion: mamC, E order pMD19T-vector.<br/> 6. Transform: transform the ligastion product into Top 10.<br/>7. Bacterial coating.<br/>8. Ligastion：Promoter - RBS order mamQ, promoter-RBS order mamB.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial culturing, including control group and all experimental groups.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | <section id="Week11"> | + | <section id="Model iGEM in Beijing"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week11</h1> | + | <h1>Model iGEM in Beijing</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>Septembert 1st</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> September 7, we went to Beijing to Participate in the Model iGEM, organized by Peking iGEM team. The Model iGEM is aimed at providing a chance for communication between Chinese iGEM teams. We showed our idea about synthetic biology with other teams, including Peking, BIT, Tianjin, NJU-China, Fudan.</font> </p><br/> |
| - | </font></p>
| + | <div class=" span4"><img src="https://static.igem.org/mediawiki/2013/ 2/ 22/Ouc- 15.jpg" height=" 600" width=" 600" /></div> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid minprep.<br/>2. Enzyme digestion and electrophoresis examining.<br/>3. Recombination. | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamL, pSB1C3 vector ).<br/>2.Transform: transform promoter-RBS-mamB, promoter-RBS-mamQ, mamK-PSB1C3 into Top10.<br/>3. PCR: use PCR to detect mamK-PSB1C3, 25ul system.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 2nd</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Electrophoresis: mamL1~20, 3, 12, 14. gel is good.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/ 9/ 94/Ouc- week14.jpg" height=" 400" width=" 300" / ><br/>Electrophoresis of mamL<br/>2. Pick bacteria: pick bacteria we want (promoter-RBS-mamB, promoter-RBS-mamQ).<br/>3. PCR: use PCR to detect promoter-RBS-mamB, promoter-RBS-mamQ. | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 3rd</h4>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Grow bacteria in the culture plates.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterial coating: mamK-PSB1C3.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 4th</h4>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamK-PSB1C3).<br/>2. PCR: use PCR to detect (mamK-PSB1C3).<br/>3. Electrophoresis: mamK-PSB1C3 number5, gel is good, others are bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick up the single colony, PCR, run gel and sequencing.<br/>2. Miniprep of B0015 and four experiment groups.<br/>3. Cultured cells of PSB1C3 for making the backbone.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 5th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep the PSB1C3.<br/>2. Enzyme digestion: B0015 with EcoR I and SpeI; the experiment group 1 use XbaI and PstI; the other experiments group use EcoR I and XbaI; the PSB1C3 with EcoRI and PstI.<br/>3. Gel extraction PSB1C3 and the experiment group 1.<br/>4. Recombination.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamK-PSB1C3).<br/>2. PCR: use PCR to detect (mamK-PSB1C3).<br/>3. Electrophoresis: gel is bad.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 6th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Transformation.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | <section id="Week12"> | + | <section id="Seminar with Mr. Tong Zhe, Founder of One-man University"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week12</h1> | + | <h1>Seminar with Mr. Tong Zhe, Founder of One-man University</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>September 9th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px">One-man University is an online university founded by Mr. Tong Zhe, based on the popular social network, renren.com. Recently, it’s been a big hit in China, renewing peoples’ views on learning institutions and education. |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick up single colony, PCR, and sequencing. | + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. PCR: use PCR to clone mamC.<br/>2. Electrophoresis: gel is bad.<br/>3. Enzyme digestion: enzyme digestion of mamI, mamQ; 25ul system, use Xba1, Pst1.<br/>4. Electrophoresis: gel is good.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 10th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Culture the experiment group 1.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Pick bacteria: pick bacteria we want (mamQ1~40, mamI1~10).<br/>2. PCR: use PCR to detect mamQ, mamI.<br/>3. Electrophoresis: gel is good.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/c/c2/Ouc-week15.jpg" height="400" width="300" /><br/>Electrophoresis of mamI<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 11st</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qiu Wang
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Miniprep and enzyme digestion with EcoR I and XbaI.<br/>2. Gel extraction.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 12th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Bacterination: Bacterinate E.coli with pSB2K3-RFP into tubes of 10ml.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. TEM (Electron microscopy) of Magnetospirillum Magneticum AMB-1.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/a/af/Ouc-week16.jpg" height="400" width="300" /><br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 13th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid extraction of pSB2K3 for 5 tubes.<br/>2. Enzyme digestion: enzyme digestion of pSB2K3 for 50ul system, use EcoR1, Pst1.<br/>3. Gel recovery: gel recovery of the product of enzyme digestion.<br/>
| + | |
| - | 4. Electrophoresis: gel is good.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 14th</h4>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xue Sun
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Inoculate the cell to culture plates, containing the control group, experiment 3 and experiment 4.
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| | | | |
| | + | It was an honor to invite Mr. Tong Zhe to give us a seminar on how to learn in the course of doing research. He gave us a talk and answered our questions about scientific research. It’s a real inspiration for us about how to educate undergraduate students to learn by themselves and for themselves. </font></p> |
| | + | <br/> |
| | + | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/a/ae/Ouc-19.jpg" height="300" width="300" /></div> |
| | + | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/a/a6/Ouc-18.jpg" height="300" width="300" /></div> |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
| - | <section id="Week13"> | + | <section id="Communicate with Tsinghua University"> |
| | <div class="page-header"> | | <div class="page-header"> |
| - | <h1>Week13</h1> | + | <h1>Communicate with Tsinghua University</h1> |
| | </div> | | </div> |
| | + | |
| | <div class="row"> | | <div class="row"> |
| | | | |
| | <div class="span9"> | | <div class="span9"> |
| | <div class="row"> | | <div class="row"> |
| - | <h4>September 15th</h4>
| + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Yu Wang | + | <div class="span9"><p style="font-weight:normal;"><font size="3px"> Communication and collaboration with other iGEM teams is another important part of iGEM. We help Tsinghua University for constructing and charactering these folowing circuit. And also, In 2011 iGEM competition, we have studied Quorum-Sensing, so we provide some AHLs they required for further experiment.</font></p> |
| - | </font></p>
| + | <br/> |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Fluorescence detection. | + | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/5/59/Ouc-16.jpg" height="300" width="300" /></div> |
| - | </font></p></div>
| + | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/44/Ouc-17.jpg" height="300" width="300" /></div> |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Qianyun Lu | + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Microscopy of Magnetospirillum magneticum AMB-1.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/e/e3/Ouc-week17.jpg" height="400" width="300" /><br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Xiaodong Zhong
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Magnetic analysis: detect the magnetism of Magnetospirillum magneticum AMB-1.
| + | |
| - | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/e/e7/Ouc-week18.jpg" height="400" width="300" /></div> | + | |
| - | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/4/44/Ouc-week19.jpg" height="400" width="300" /></div><br/><br/> | + | |
| - | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/f/f6/Ouc-week20.jpg" height="400" width="300" /></div>
| + | |
| - | <div class="span4"><img src="https://static.igem.org/mediawiki/2013/6/6f/Ouc-week21.jpg" height="400" width="300" /></div>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | <h4>September 20th</h4>
| + | |
| - | | + | |
| - | | + | |
| - | <div class="span9"><p style="font-weight:normal;"><font size="3px">Person:Kaili Qin
| + | |
| - | </font></p>
| + | |
| - | <p style="font-weight:normal;"><font size="3px">Experiment:<br/>1. Plasmid miniprep: plasmid Extraction of double expression plasmid for 9 tubes.<br/>2. Electrophoresis: gel is good.<br/>
| + | |
| - | <img src="https://static.igem.org/mediawiki/2013/1/13/Ouc-week22.jpg" height="400" width="300" /><br/>Electrophoresis of double expression plasmid<br/>
| + | |
| - | </font></p></div>
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| | </div> | | </div> |
| - | </div>
| + | </div> |
| - | </div>
| + | </div> |
| - | <br /><br /></br>
| + | </div><br /><br /></br> |
| | </section> | | </section> |
| | | | |
"
