Team:OUC-China/Lab note

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Lab-note





      

        

		
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Week1

          

		  
June 26th

		  

		  
  

    

      
      
Person: Wenjun Wang

	  　
Experiment:

	     1. Get the AMB-1 bacteria strain from Nanjing University.
2. Keep the strain in 30℃.

	  
 

	  

	  

	  

 







		    

	  

            
Week2

          

		  
June 30th

		  

		  
  

    

      
      
Person: Wengjun Wang
　　　

      
Experiment:

1. PCR: Get 16srDNA sequence from AMB-1 bacteria genome to detect the strain.

2. Agarose electrophoresis: detect the PCR product.

3. Transform: transform the ligastion product into Top 10 to prepare for sequence.
　　　

	  

	  

	  

 





	    	 
	  
	  

	  

            
Week3

          

		  
July 13th ~ 15th

		  
		  

		  
  

    

      
     
 
Person: Wenjun Wang

	  　
Experiment:

	     1. Get the AMB-1 bacteria strain from Nanjing University.
2. Keep the strain in 30℃.

	 
 

	  
 

	  
 

	  
 

	  
 

	  
 

	  
 

	  
 

    

  











	      

	  

            
Week4

          

		  

		  
  

    

       
June 30th

      
Person: Kaili Qin
　　　

      
Experiment:

　　1. Equip the medium: Equip the medium of Magnetospirillum Magneticum AMB-1.

　　　

       
July 20th

	   
Person: Wenjun Wang
　　　

      
Experiment:
1. PCR: Get mamJ sequence from PCR product to finish the Over-Lap PCR. 

    2. Agarose electrophoresis: detect the plasmid PCR product.

	3. Transform: transform the ligastion product into Top 10 to prepare for sequence.

	4. PCR: Get mamK sequence from PCR product to finish the Over-Lap PCR. 

	5. Agarose electrophoresis: detect the PCR product.

	6. Product purification: purify the PCR product.

	7. PCR: Get mamAB sequence from AMB-1 bacteria genome.
　　　

    
Person: Qianyun Lu
　　　

      
1. Culture the E.coli cells: Psb1c3.

　　　
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