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Team:Paris Bettencourt/Notebook/Drug Screening/Friday 19th July.html
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<a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Drug_Screen" target="_blank" class="tbnotelogo DSlogo"> ASDF </a> | <a href="https://2013.igem.org/Team:Paris_Bettencourt/Project/Drug_Screen" target="_blank" class="tbnotelogo DSlogo"> ASDF </a> | ||
<div style="clear: both;"></div> | <div style="clear: both;"></div> | ||
| - | <h3> | + | <h3>Friday 19<sup>th</sup> July</h3> |
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| - | <li>< | + | <li><font color="#EE0F29">sD012/sD013</font>=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes |
| - | <li><FONT COLOR="# | + | <li><FONT COLOR="#EE0F29">sD016/sD017</FONT>=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin |
| - | <li><FONT COLOR="# | + | <li><FONT COLOR="#EE0F29">sD014/sD015</FONT>=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol |
</ul> | </ul> | ||
<br><b>Glycerol Stocks were made of the above mentioned strains</b> | <br><b>Glycerol Stocks were made of the above mentioned strains</b> | ||
| - | Single colonies from agar | + | <br> 2 Single colonies from each agar plate of above mentioned strains were picked and used to inoculate 5ml LB broth overnight. <br> 750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.<br> Two sets of Glycerol stocks were used for each of sD001+pD004, sD001+sD004+sD005, sD001+sD004+sD006; <br>one set was frozen at -20ºC and the other set was frozen at -80ºC. |
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Latest revision as of 10:31, 13 August 2013
Drug Screening
ASDFFriday 19th July
TRANSFORMATIONS WORKED!!! cells were stocked and entered in catalog.
- sD012/sD013=sD001+pD004+pD005 E.coli:ΔcysI, Δfpr, ΔydbK:: soFD, zmSIR ;Chloramphenicol ,zmFNR; Spectinomycin- with all three genes
- sD016/sD017=sD001+pD004 E.coli:ΔcysI, Δfpr, ΔydbK:: ,zmFNR; Spectinomycin
- sD014/sD015=sD001+pD004+pD006 E.coli: ΔcysI, Δfpr, ΔydbK::zmFNR; Spectinomycin, zmSIR; Chloramphenicol
Glycerol Stocks were made of the above mentioned strains
2 Single colonies from each agar plate of above mentioned strains were picked and used to inoculate 5ml LB broth overnight.
750ml of overnight culture was added to 250ml of 60% glycerol in a cryotube.
Two sets of Glycerol stocks were used for each of sD001+pD004, sD001+sD004+sD005, sD001+sD004+sD006;
one set was frozen at -20ºC and the other set was frozen at -80ºC.
