Team:Paris Bettencourt/Notebook/Drug Screening/Friday 30th August.html

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   Confirmation of P1 phage transduction:

Colony PCR was used on patched colonies from the p1 phage transduction. 

   PCR confirmed that CysI was deleted in BL21(AI) and replaced with a Kanomycin resistance gene.

</p> <img src="PCR_confirmation_of_BL21AIdeleted_CysI.jpg" width="200" height="200">

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