Team:Paris Bettencourt/Notebook/Drug Screening/Friday 6th September.html

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Phage Sensor

Friday 6th September

Place your twit here

Digestions: linearized pSB1C3, linearized pSB1A3, SPCR2, SPCR3, SPCR11

Reagent Volume
Purified PCR Product 16 µl
H2OH2O 0 µl
10x FastDigest Buffer 2 µl
FastDigest Enzyme 1 1 µl
cFastDigest Enzyme 2 1 µl
Total Volume 4 µl


SPCR5, pSB1C3: XbaI/SpeI
SPCR11, pSB1A3, pSB1C3: EcoRI/PstI
SPCR13, pSB1A3, pSB1C3: EcoRI/PstI
SPCR2, pSB1A3, pSB1C3: EcoRI/PstI

1h at 37° shaking

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