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From 2013.igem.org

   P1 phage transduction

 
  The p1 phage lysate was prepared as described in the P1 phage transduction protocol.

 
  BL21 (DE3) ΔCysI Δfpr ΔydbK was grown in 5ml Lb.
   
One colony was discovered in transformed plate.  This colony was re-streaked and grown overnight.

the ligation product from overnight ligation was used to transorm NEB cc cells by heatshock
5ul plate gave a few colonies
10ul plate gave many colonies </div>